GenBank-Updates@genbank.bio.net (09/17/90)
LOCUS YSCTGYP 104 bp ss-RNA PLN 17-SEP-1990
DEFINITION Yeast (S.cerevisiae) Tyr-tRNA gene.
ACCESSION K02839
KEYWORDS transfer RNA; transfer RNA-Tyr.
SOURCE Yeast (S.cerevisiae) RNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 104)
AUTHORS Nishikura,K. and De Robertis,E.M.
TITLE RNA processing in microinjected Xenopus oocytes: Sequential
addition of base modifications in a spliced transfer RNA
JOURNAL J. Mol. Biol. 145, 405-420 (1981)
STANDARD full staff_review
COMMENT The 3' terminal "cca" is added at the 92 base precurser stage. The
modified base "u**," in the Sites Table, is an unknown "u"
modification.
FEATURES from to/span description
RNA 1 104 Tyr-tRNA precurser RNA (alt.)
RNA 13 102 Tyr-tRNA precurser RNA (alt.)
tRNA 13 52 Tyr-tRNA, exon 1
67 102 Tyr-tRNA, exon 2
IVS 53 66 tRNA intron (no splice consensus)
modified 19 19 m2g (92 bp precurser)
modified 23 23 m2g (92 bp precurser; partially in 104 bp
precurser)
modified 29 29 d (92 bp precurser)
modified 30 30 d (92 bp precurser)
modified 33 33 d (92 bp precurser)
modified 34 34 d (92 bp precurser)
modified 35 35 u** (92 bp precurser)
modified 41 41 m22g (92 bp precurser; partially in 104 bp
precurser)
anticdn 49 51 Tyr-tRNA anticodon gta
modified 50 50 p (both precursers)
modified 68 68 p (92 bp precurser)
modified 76 76 p (92 bp precurser)
modified 77 77 m5c (both precursers)
modified 83 83 p in one species; t in another species (both
precusers)
modified 84 84 p (both precursers)
modified 87 87 m1a (both precursers)
BASE COUNT 26 a 23 c 26 g 29 t
ORIGIN 5' end of 104 bp precurser.
1 atcagttaat tgactcttgg tagccaagtt ggtttaaggc gcaagactgt aatttaccac
61 tacgaaatct tgagatcggg cgttcgactc gcccccggga gatt
//