GenBank-Updates@genbank.bio.net (09/17/90)
LOCUS DDITGV 821 bp ds-DNA INV 17-SEP-1990
DEFINITION Slime mold (D.discoideum) Val-tRNA gene and His-tRNA pseudogene.
ACCESSION X01763 M24065 M25954
KEYWORDS pseudogene; transfer RNA; transfer RNA-Val.
SOURCE Slime mold (D.discoideum) DNA, clone pDDt2.
ORGANISM Dictyostelium discoideum
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
Rhizopoda; Eumycetozoa; Dictyostelia; Dictyosteliida;
Dictyosteliidae.
REFERENCE 1 (bases 1 to 821)
AUTHORS Dingermann,T., Bertling,W., Pistel,F. and Amon,E.
TITLE Characterization of a Dictyostelium discoideum DNA fragment coding
for a putative Val-tRNA-GUU gene: Evidence for a single
transcription unit consisting of two overlapping class III genes
JOURNAL Eur. J. Biochem. 146, 449-458 (1985)
STANDARD simple staff_review
REFERENCE 2 (bases 1 to 195)
AUTHORS Dingermann,T., Amon-Boehm,E., Bertling,W., Marschalek,R. and
Nerke,K.
TITLE A family of non-allelic Val-tRNA-GUU genes from the cellular slime
mold Dictyostelium discoideum
JOURNAL Gene 73, 373-384 (1988)
STANDARD full staff_entry
REFERENCE 3 (bases 3 to 88)
AUTHORS Dingermann,T. and Nerke,K.
TITLE Primer extension analysis of tRNA gene transcripts synthesized in
vitro and in vivo
JOURNAL Anal. Biochem. 162, 466-475 (1987)
STANDARD simple staff_entry
FEATURES from to/span description
tRNA 62 135 Val-tRNA (put.)
RNA 54 > 135 Val-tRNA precursor start
RNA 57 > 135 Val-tRNA precursor start
site < 1 87 His-tRNA pseudogene (put.)
BASE COUNT 335 a 98 c 115 g 273 t
ORIGIN 1 bp upstream of HindIII site.
1 aagcttcaat gatagaaatt taaaacgggt atgagttatt taacatgaaa aatatcgatt
61 agttcggatg gtgtagtcgg ttatcacgaa tccttaacac ggattaggtc gtgggttcga
121 ttcccgctct gaatacaaac taatactttt cccaaagaca aattttcata gtcaaaagtt
181 cgattccaga agaatttgca gtctttgaaa aaaaaaagta atttagcaat ttatttaaaa
241 ttttaatacc ttttaaaatc atttaattta tataatttag tttgttttat tattatattt
301 catcctataa aaacataaaa ccaaaaaaat tattgaattt gatggttaaa tactaattag
361 ttaatatttt tttttttttt ttttttttaa taaaatgttg gtgaaatttt aatacacaaa
421 tccaataaaa aaaaaaaatt taataaaaaa cagcaaagat caaaaaaaaa ataaacaaaa
481 taaaaaatat aaatgatagg aatgaattta aagatttatt ttattaaaac atttagatca
541 ataattatta attattttct aagaatatca gcctaaagag ttatttaaaa taaaaggcca
601 gagaaattaa aactgcagtt aaacaaaatg atgaaataat tcttaaactt gagtttggat
661 gatcaccatc agctgaagta tcatcaccag ttgcagatcc agtagctgaa ccagttgaag
721 aagtagtagc accagttgaa gaagtagcag aaccagtagt ggttggaact ggtttatcaa
781 cttcaccaga ttgatagttt gaagcaccta atgttggatc c
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