GenBank-Updates@genbank.bio.net (09/18/90)
LOCUS STMHISOPA 3981 bp ds-DNA BCT 18-SEP-1990
DEFINITION S.coelicolor histidine biosynthesis operon encoding hisD, partial
cds., and hisC, hisB, hisH, and hisA genes, complete cds.
ACCESSION M31628
KEYWORDS L-histidinol:NAD oxidoreductase; glutamine amidotransferase;
histidinol-phosphate aminotransferase;
imidazoleglycerol-phosphate dehydratase; isomerase.
SOURCE S.coelicolor (strain A3(2)) DNA.
ORGANISM Streptomyces coelicolor
Prokaryota; Bacteria; Firmicutes; Streptomycetaceae.
REFERENCE 1 (bases 1 to 3981)
AUTHORS Limauro,D., Avitabile,A., Cappellano,M., Puglia,A.M. and Bruni,C.B.
TITLE Cloning and characterization of the histidine biosynthetic gene
cluster of Streptomyces coelicolor A3(2)
JOURNAL Gene 90, 31-41 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by C.B.Bruni, 25-JAN-1990.
FEATURES from to/span description
pept < 1 468 L-histidinol:NAD oxidoreductase (hisD) (EC
1.1.1.23)
pept 465 1574 histidinol-phosphate amidotransferase (hisC)
(gtg start codon) (EC 2.6.1.9)
pept 1571 2164 imidazoleglycerol-phosphate dehydratase (hisB)
(EC 4.2.1.19)
pept 2168 2332 unidentified ORF1
pept 2329 2997 glutamine amidotransferase (hisH) (ttg start)
(EC 5.3.1.16)
pept 3015 3737 isomerase (hisA)
pept 3754 > 3981 unidentified ORF2 (gtg start)
BASE COUNT 619 a 1498 c 1324 g 540 t
ORIGIN Map position 12 o'clock.
1 gagctccagc cgcaggtcga ggccaccaag cacatcgagg accggatccg tccggccctg
61 gccggccgcc agtccgcgat cgtcctcgtc gacggcctgg acgagggcct gcgggtcgtg
121 gacgcctacg gcgccgagca cctggagatc cagaccgccg acgccgcggc cgtcgccgac
181 cgcgtcaaga acgccggcgc gatcttcgtc ggcccctggg cgcccgtctc gctcggcgac
241 tacgcggccg ggtccaacca cgtcctgccc accggcggct gcgcctgcca ctcctcgggc
301 ctgtccgtgc agtccttcct gcgcggcatc cacatcgtcg actacacgcg cgacgcgctc
361 gccgaggtcg cacggcacgt ggtgacgctg gccgaggcgg aggacctgcc cgcgcacggc
421 gcggcgatca aggcgaggtt cgagtggaag gtcccggaga gcaagtgacg ttcggcatcg
481 acgatctccc cgtacgggac gagctgcgcg gcaagtcccc ctacggcgcg ccccaactgg
541 acgtgccggt acggctgaac accaacgaga acccctaccc gctgcccgag gcgctggtcg
601 agcggatcgc cgagcgggtc cgcgaggccg cccgcgacct caaccgctac cccgaccggg
661 acgcggtcga gctgcgcact caactggccc ggtacctgac ggacacctcg ggacacccgc
721 tggacgtgag caacgtctgg gcggccaacg gctccaacga ggtcatccag caactgctgc
781 agaccttcgg cgggccgggc cgcaccgcga tcggcttcga gccgtcgtac tcgatgcacg
841 gcctcatcgc gcgcggcacc ggcaccggct ggatctccgg gccgcgccac gaggacttca
901 ccatcgacgt gcccgccgcc acgcgggcga tcgacgagca ccgcccggac gtcgtcttca
961 tcaccacccc caacaacccc acgggaaccg cggtcccggc cgagacggtc ctcgccctgt
1021 acgaggccgc ccaggcggcg aagccgtcca tggtcgtggt ggacgaggcg tacatcgagt
1081 tcagccacgg cgcctcgctg ctgccgctgc tcgacgggcg gccgaacctc gtcgtctccc
1141 gcacgatgtc gaaggcgttc ggcgcggcgg gcctgcgcct cggctacctg gccgcgcacc
1201 cggccgtcgt cgacgcggtc cagctcgtac ggctgccgta ccacctgtcg gccgtgacgc
1261 aggcgaccgc gctggccgcc ctggagcaca cggacacgct gctgaagtac gtcgagcagc
1321 tgaagaccga gcgggaccgg ctggtcgccg aactgcgcgc catcggctac gcggtgaccg
1381 agtccgacgc gaacttcgtc cagttcgggc ggttcgcgga ctcccacgcg acctggcgga
1441 agatcctcga ccggggcgtc ctggtccggg acaacggcgt gccggggtgg ctgcgggtca
1501 ccgccggcac tccggaggag aacgacgcgt tcctcgacgc ggtacgtgaa gtcaagaagg
1561 agcagcacac atgagccgcg tagggcgcgt ggagcgcacc accaaggaga cctcggtcct
1621 cgtcgagatc gacctcgacg gcaccggcaa gaccgacatc gccaccggcg tcggcttcta
1681 cgaccacatg ctcgaccagc tcggccggca cggtctgttc gacctgaccg tgaagaccga
1741 cggcgacctg cacatcgact cccaccacac catcgaggac accgccctcg cgctgggcgc
1801 cgccttcagg caggccctcg gcgacaaggt gggcatctac cgcttcggca actgcacggt
1861 cccgctggac gagtccctcg cccaggtcac cgtcgacctc tccggccgcc cctacctcgt
1921 gcacaccgag cccgagaaca tggcgccgat gatcggcgag tacgacgtga cgatgacccg
1981 gcacatcctg gagtccttcg tcgcccaggc ccaggtcgcc ctgcacgtcc acgtgcccta
2041 cgggcgcaac gcgcaccaca tcgtggagtg ccagttcaag gcgctggccc gggccctgcg
2101 ctacgcctcg gagcgcgacc cgcgcgcggc cggcatcctg ccttccacga agggcgcgct
2161 gtaacccatg aacggtacgt ccaccgcact gatcgtcgtc ggcctcttct tcctcggcgg
2221 catctactcc ttcgccaagc agcagatgcc caagggcctg atcgtgctgc tctccatcgg
2281 cgcgggcatg tgcctgctcg cgggcgtcct gaggctggag gtctggagtt gaccgccgca
2341 gtccccgccg gcgccaccgg ccgcgccaag aaggtcgtcg tcttcgacta cggcttcggc
2401 aacgtccgct ccgccgagcg cgccctcgcg cgcgcggggg ccgacgtcga gatcacccgt
2461 gactacgaca aggccatgaa cgccgacgga ctgctggtcc ccggcgtcgg cgccttcgcc
2521 gcctgcatgg agggcctcaa ggccgcccgg ggcgactgga tcgtcgaccg ccgcctgtcc
2581 ggcgggcgcc cggtcatggg catctgtgtc ggcatgcaga tcctcttctc gcgcggcatc
2641 gagcacgacg tggaggccga gggcctggac gagtggcccg gcacggtcgg acccctcgag
2701 gccgacgtcg tgccccacat gggctggaac acggtcgagg caccggccga ctcccagctc
2761 ttcgccggcc tggacgcgga cgcccgcttc tacttcgtgc actcctacgc cgtccacgag
2821 tggacccagg agtcgcacaa cccgctgatc gccgagccca gggtcacctg gtccacccac
2881 ggcaagccct tcgtggccgc cgtggagaac ggtgcgctgt gggccaccca gttccacccc
2941 gagaagtccg gcgacgccgg agcccagctc ctcaccaact ggatcgaaac cctctagaga
3001 tgagcccggt agagatgagc aagctcgaac tcctccccgc cgtcgacgtc cgcgacggcc
3061 aggccgtccg cctcgtgcac ggcgagtccg gcaccgagac ctcctacggc tccccgctgg
3121 aggccgccct cgcctggcag cgctccggcg ccgagtggct gcacctggtg gacctggacg
3181 cggcgttcgg gaccggggac aaccgcgcgc tgatcgccga ggtcgcgcag gccatggaca
3241 tcaaggtgga gctgtccggc ggcatccgcg acgacgacac cctcgccgcc gcgctcgcca
3301 ccggctgtac ccgggtgaac ctgggcacgg ccgccctgga gacccccgag tgggtcgcca
3361 aggtcatcgc cgagcacggc gacaagatcg cggtcggcct ggacgtacgg ggcaccacgc
3421 tccgcggccg cggctggacc cgcgacggcg gcgacctcta cgagacgctg gaccgcctga
3481 acaaggaggg ctgcgcccgc tacgtcgtca ccgacatcgc caaggacggc accctccagg
3541 gccccaacct ggagctgctg aagaacgtct gcgcggccac cgaccgcccg gtcgtggcgt
3601 cgggcggcgt ctcctccctg gacgacctgc gcgcgatcgc cgggctcgtc ccggccggtg
3661 tcgagggggc catcgtcggg aaggccctgt acgcgaaggc gttcaccctg gaagaagcct
3721 tggaggctac gtcgtgaaat cggacaccgt gtcatgacat ccgaagccgt gcggcgcgtg
3781 cagagcggga gtccctggga agagtccttc ggtttcgccc gcgccgtggc ggcgggtgat
3841 cgggtcatcg tggcgggcac caccgccttc aagggcgaca tgctctacgg cgagggcccc
3901 ccgtacgaac agaccaaggt ggccttcggc accgccgtcg aggcgatcgc cgagttcggg
3961 ctcggcatcg agtccgtgat c
//