GenBank-Updates@genbank.bio.net (09/18/90)
LOCUS ACCP322P 1337 bp ds-DNA SYN 18-SEP-1990
DEFINITION Synthetic plasmid pWH1266 origin of replication (ori) region.
ACCESSION M36473
KEYWORDS .
SOURCE A.calcoaceticus lwoffi plasmid and pBR322 DNA, clone pWH1266.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 1337)
AUTHORS Hunger,M., Schmucker,R., Kishan,V. and Hillen,W.
TITLE Analysis and nucleotide sequence of an origin of DNA replication in
Acinetobacter calcoaceticus and its use for Escherichia coli
shuttle plasmids
JOURNAL Gene 87, 45-51 (1990)
STANDARD simple staff_entry
FEATURES from to/span description
orgrpl 310 337 origin of replication
BASE COUNT 447 a 229 c 251 g 410 t
ORIGIN
1 gatcgtagaa atatctatga ttatcttgaa gaacgcaacc ctatagcagc tattgaaatt
61 gatgatttaa ttgaagaaaa gacagattta gttgttgata atcgactgat ggggcgcaca
121 ggcagacaga aagatactag ggagttagtg atacatccgc attatgtggt tgtatatgac
181 atcactgata taatacggat actcagagtg ctacacacat cgcaggagtg gtcatgactt
241 actcatgtac tttggattat ttagtgttat aaaatcctga tttataaatt ttttttgtta
301 aaaaagataa aagccccttg caattgcttg gggctttacc gtaatttatg gggtacagat
361 cttcgatact gacatatcgg caatcgaaag cattaaggtt tgacgaccgc taatgatttc
421 accacagggg cttaatgtac ctgtcttaaa ttctaaggtt ttaactcgct ttgtcaagca
481 tagaccccaa aaatttagcc aatgtctgta actcaatctg tccatgtgtg ggtgatgagg
541 tacagtgacg ctagcacaca tcggaaaaac gctattacta ggggaactga acagagtagc
601 ggacgcaatg agtagtcatt taattggcgg ttatgagcgt gttcaggcgg tgctatcaat
661 cgtaatcata acagtggcag cttgatacag tgatgtcatc cctgatgcga aagcgaccga
721 ccgacggtac atcgaatggg aatactttag ggtgattttt aagaatcgct ctagggtgag
781 tatttcccat tcagctctgc tccctccctc tggtacttta atcaaaagca ctactaaaca
841 tatgttttta aataaaaaat attgatatag agataatatt agtaagaata attaaacaat
901 tgaatataga taaatcattg ttaaataaag attaattatt aaaatgaatg tatacttata
961 tataaatcaa tgatttaaaa tatttgataa agaaaacttt tcaaaaaaaa tataattgag
1021 attgtgtcat ttcggtcaat tcttaatatg ttccacgcaa gttttagcta tggtgctaaa
1081 cagaaatttg ctgaaaaaga acttttcact gaactggtta aaatgtaagc agcctgagag
1141 ccgccaaaaa ttttaaaaac aaaccgcctt aatcatcttc aaaaaatacc tctaaaacct
1201 caccatttgc gttttaagac ccatatttca tcctgccctt atgttcccat gctgatagct
1261 ataaagtgtc tgtaatcgct tcctatgacg ttctaggctg ttgataactt ttggaacaac
1321 gcaaaatgtt aaaatcc
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