GenBank-Updates@genbank.bio.net (09/18/90)
LOCUS TRHTCS 1227 bp ds-DNA PLN 18-SEP-1990
DEFINITION T.kirilowii alpha-trichosanthin (alpha-TCS) gene, complete cds.
ACCESSION J05434
KEYWORDS alpha-trichosanthin; ribosome-inactivating protein;
type I ribosome-inactivating protein.
SOURCE T.kirilowii (strain Maximowicz) leaf DNA, clone pQ21D.
ORGANISM Trichosanthes kirilowii
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Dilleniidae; Violales; Cucurbitaceae.
REFERENCE 1 (bases 1 to 1227)
AUTHORS Chow,T., Feldman,R.A., Lovett,M. and Piatak,M.
TITLE Isolation and DNA sequence of a gene encoding alpha-trichosanthin,
a type I ribosome-inactivating protein
JOURNAL J. Biol. Chem. 265, 8670-8674 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by M.Piatak, 15-MAR-1990, for release after publication.
FEATURES from to/span description
pept 340 1209 alpha-trichosanthin precursor
/function="Inactivates eukaryotic 60S
ribosomal subunits"
sigp 340 408 alpha-trichosanthin signal peptide
matp 409 1149 alpha-trichosanthin
signal 25 30 TATA box (put.)
signal 168 173 TATA box (put.)
signal 238 243 TATA box (put.)
signal 283 288 TATA box (put.)
BASE COUNT 402 a 250 c 223 g 352 t
ORIGIN
1 gaattcaaat attttctgaa taaatataaa attcattgta gagaaatgat gagaaaacaa
61 gaacaatttt caaaaacaaa aaataaaaaa caaatggttt atcaaagaag gcccgaattt
121 attatcaaaa gcgaaagtta ataatatcta aaaaaaaact attaccttat aagaagctat
181 tacctagatg gcataagatc atacttttat ttttgattta gactagaaat acactaatat
241 attgtggaca tagccagaca acgattagat ggcatacctc tctataaaaa ccacagcttg
301 agatgctcca atggcatcca aattcctcaa gtcaaaaaga tgatcagatt cttagtcctc
361 tctttgctaa ttctcaccct cttcctaaca actcctgctg tggagggcga tgttagcttc
421 cgtttatcag gtgcaacaag cagttcctat ggagttttca tttcaaatct gagaaaagct
481 cttccaaatg aaaggaaact gtacgatatc cctctgttac gttcctctct tccaggttct
541 caacgctacg cattgatcca tctcacaaat tacgccgatg aaaccatttc agtggccata
601 gacgtaacga acgtctatat tatgggatat cgcgctggcg atacatccta ttttttcaac
661 gaggcttctg caacagaagc tgcaaaatat gtattcaaag acgctatgcg aaaagttacg
721 cttccatatt ctggcaatta cgaaaggctt caaactgctg cgggcaaaat aagggaaaat
781 attccgcttg gactcccagc tttggacagt gccattacca ctttgtttta ctacaacgcc
841 aattctgctg cgtcggcact tatggtactc attcagtcga cgtctgaggc tgcgaggtat
901 aaatttattg agcaacaaat tgggaagcgc gttgacaaaa ccttcctacc aagtttagca
961 attataagtt tggaaaatag ttggtctgct ctctccaagc aaattcagat agcgagtact
1021 aataatggac agtttgaaac tcctgttgtg cttataaatg ctcaaaacca acgagtcatg
1081 ataaccaatg ttgatgctgg agttgtaacc tccaacatcg cgttgctgct gaatcgaaac
1141 aatatggcag ccatggatga cgatgttcct atgacacaga gctttggatg tggaagttat
1201 gctatttagt gtaacttcaa gctacgt
//