GenBank-Updates@genbank.bio.net (09/20/90)
LOCUS MSG32KDA 1357 bp ds-DNA BCT 20-SEP-1990
DEFINITION Mycobacterium tuberculosis 32 kDa antigen gene.
ACCESSION M27016 X53898
KEYWORDS antigen; secreted protein.
SOURCE M.tuberculosis DNA.
ORGANISM Mycobacterium tuberculosis
Prokaryota; Bacteria; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE 1 (bases 1 to 1357)
AUTHORS Borremans,M., de Wit,L., Volckaert,G., Ooms,J., de Bruyn,J.,
Huygen,K., van Vooren,J.-P., Stelandre,M., Verhofstadt,R. and
Content,J.
TITLE Cloning, sequence determination, and expression of a 32-
Kilodalton-protein gene of Mycobacterium tuberculosis
JOURNAL Infect. Immun. 57, 3123-3130 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by J.Content 07-SEP-1989, for release after publication.
N-term. of the mature 32 kDa protein was determined experimentally.
N-term. of the signal peptide was set referring to the most
stringent ribosome-binding site [1].
FEATURES from to/span description
pept 233 1243 32 kDa antigen protein precursor
sigp 233 358 put. signal peptide (AA -42 to -1)
matp 359 1240 mat. 32 kDa antigen (AA 1 - 294)
BASE COUNT 235 a 430 c 444 g 238 t 10 others
ORIGIN
1 cgacacatgc ccagacactg cggaaatgcc accttcaggc cgtcgcgtcg gtcccgaatt
61 ggccgtgaac gaccgccgga taagggtttc ggcggtgcgc ttgatgcggg tggacgccca
121 agttgtggtt gactacacga gcactgccgg gcccagcgcc tgcagtctga cctaattcag
181 gatgcgccca aacatgcatg gatgcgttga gatgaggatg agggaagcaa gaatgcagct
241 tgttgacagg gttcgtggcg ccgtcacggg tatgtcgcgt cgactcgtgg tcggggccgt
301 cgcgcgccta gtgtcgggtc tggtcggcgc cgtcggtggc acggcgaccg cgggggcatt
361 ttcccggccg ggcttgccgg tggagtacct gcaggtgccg tcgccgtcga tgggccgtga
421 catcaaggtc caattccaaa gtggtggtgc caactcgccc gccctgtacc tgctcgacgg
481 cctgcgcgcg caggacgact tcagcggctg ggacatcaac accccggcgt tcgagtggta
541 cgaccagtcg ggcctgtcgg tggtcatgcc ggtgggtggc cagtcaagct tctactccga
601 ctggtaccag cccgcctgcc gcaaggccgg ttgccagact tacaagtggg agaccttcct
661 gaccagcgag ctgccggggt ggctgcaggc caacaggcac gtcaagccca ccggaagcgc
721 cgtcgtcggt ctttcgatgg ctgcttcttc ggcgctgacg ctggcgatct atcaccccca
781 gcagttcgtc tacgcgggag cgatgtcggg cctgttggac ccctcccagg cgatgggtcc
841 caccctgatc ggcctggcga tgggtgacgc tggcggctac aaggcctccg acatgtgggg
901 cccgaaggag gacccggcgt ggcagcgcaa cgacccgctg ttgaacgtcg ggaagctgat
961 cgccaacaac acccgcgtct gggtgtactg cggcaacggc aagccgtcgg atctgggtgg
1021 caacaacctg ccggccaagt tcctcgaggg cttcgtgcgg accagcaaca tcaagttcca
1081 agacgcctac aacgccggtg ggcgccacaa cggcgtgttc gacttcccgg acagcggtac
1141 gcacagctgg gagtactggg gcgcgcagct caacgctatg aagcccgacc tgcaacggca
1201 ctgggtgcca cgcccaacac cgggcccgcc gcagggcgcc tagctccgaa cagacacaac
1261 atctagcnnc ggtgaccctt gtggnncana tgtttcctaa atcccgtccc tagctcccgc
1321 ngcnnccgtg tggttagcta cctgacnnca tgggttt
//