GenBank-Updates@genbank.bio.net (09/21/90)
LOCUS DCGAMYBA 2496 bp ds-DNA BCT 21-SEP-1990
DEFINITION Dictyoglomus thermophilum amyB gene for amylase.
ACCESSION X13199 M36505
KEYWORDS amyB gene; amylase.
SOURCE D.thermophilum strain=H-6-12 DNA, clone pDA201.
ORGANISM Dictyoglomus thermophilum
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci.
REFERENCE 1 (bases 1 to 2496)
AUTHORS Horinouchi,S., Fukusumi,S., Ohshima,T. and Beppu,T.
TITLE Cloning and expression in Escherichia coli of two additional
amylase genes of a strictly anaerobic thermophile, Dictyoglomus
thermophilum, and their nucleotide sequences with extremely low
guanine-plus-cytosine-contents
JOURNAL Eur. J. Biochem. 176, 243-253 (1988)
STANDARD full staff_entry
COMMENT
FEATURES from to/span description
pept 608 2296 amylase (AA 1 - 562)
binding 594 600 pot. ribosome binding site
BASE COUNT 909 a 400 c 424 g 763 t
ORIGIN
1 cttaagtaat cattcttatc ccttattaaa ttaggtctta tattaggatc aaaggaaatt
61 aagaccttcc catgattttg agtcatcatt ttctctaata caaaacaccc tggttctctt
121 atcatagata tagagccaaa atgaataagc tcaacttttt ctggatctat taagggtata
181 tccttttctt ctaaagagac atcagcagta ttttctccgt aaaatataaa atgaggctct
241 ccttctctca agataacaaa agcaagagta gtattcttct ctgatcttaa tactaaggaa
301 atatctacgt gatttttctt cagtttttca atcaacatct ctccaaaaaa atcgtaagat
361 atcttaccaa aaaaaccaca aggtgctccc aatcttccca aagtaattgc tacattatat
421 ggcgatccac cttctttagg tatatatgct atttcaccat ttatctctat aggagtaaaa
481 tctataagtg cttcaccacc cacaataatc atgttatcat tacctcctta agaatttatg
541 tttgtataat cattataaac tatgataaaa taattaacac gagttaattt actggaggta
601 gaataaaatg atatacgatg acaaaatatt cggagattta tgccacaaag agtttttagt
661 agaaagagaa gttaaaaaac ttgaagaaat atatttagaa gaggttttac cagaagatcc
721 taagcctgaa gatgaaatag aatttacttt taactgtcca ttaaagtttc acattacttc
781 aggaaaaatt gtcaaagata acagagaaat atataccttt aatatacagg aaagaaaaac
841 tcaatggaat gatagcatat ttaactttag cgaaattatc aaaataaaaa tacctccttt
901 aaaggaaaat ggtctatatc aaatccacct ttatgagatg aatgaaaaaa tctatgagca
961 atatctctca atagacagtt ttgaagcacc cttatggagt gaggagagta taatttatca
1021 catatttatt gatagatttg ctaaagatga aaaagaagta gaatattctg aaaacctcaa
1081 agagaaatta ggaggaaacc tgaaggggat actaagccgt ttggattata tagaaaacct
1141 tgggataaat accatatgga tatctcccat ttttaaaagc acatcatatc atggttatga
1201 tattgaggat tactttgaaa ttgatcctat ttggggaaca aaagaagatt taaaaaaact
1261 tgtaagggaa gccttcaata gaggcataag aataatctta gactttgtac caaatcatat
1321 gtcatataaa aatcctatat ttcagaaagc tttaaaagac aaaaacagta atctaagaag
1381 ttggtttata tttaaaggag aagattatga aacctttttc ggtgtaaagt caatgcccaa
1441 aataaacctt aaaaataagg aagcgataga ttatataata aatgctgcca aatactggat
1501 aagagaattt ggaatttctg gatatagaat ggatcatgct acaggtcctg acattaattt
1561 ttggagtatt ttttattata atttgaaatc tgaatttcca gaaactttct attttggaga
1621 gatagtagag actccgaaag aaacaaaaaa atatgtaggc aaatttgacg gaactttaga
1681 tttttatctt tttaaaataa ttagagactt ttttataggt aaaagatggt ctaccaaaga
1741 gtttgtaaaa atgatcgatt tggaagaaaa attctatgga aataaattca agagaatttc
1801 ttttctcgaa aatcacgatt caaatagatt tctatgggtt gctaaagata agaaattact
1861 tagattagct tcaattttcc aattctcgat taatgcaata cctataatct acaatggaca
1921 agaaatggga tgtagtcagt acagagatat acttgaggga aatagaactc tacacgaaca
1981 tgcaagactt cctataccct ggagtgatga caaacaagac aaagaactta tagattttta
2041 caggcaatta gttaaaatca gaaaaagtca tccagcgctt tacaaaggta cgtttattcc
2101 tatattttca gatatgatat cttttatcaa agaaacccaa gaagaatcca tacttgtact
2161 tataaatata gaggataaag aagaaatctt taatctgaac ggtacgtaca gagatctatt
2221 tagcggaaat atttacacta attctttaaa gctaggcccg atgagtgctc acttattact
2281 tcgtatagat cactgatcct tactagatcc tctacgccgg acgcatcgtg gcggcatcac
2341 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
2401 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg ctggccccgt
2461 ggccggggga ctgttgggcg ccatctcctt gcatgc
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