GenBank-Updates@genbank.bio.net (09/22/90)
LOCUS YSCPHOM1 120 bp ds-DNA SYN 22-SEP-1990
DEFINITION Yeast (S.cerevisiae) M-1 plasmid killer toxin gene fused to PHO5
promoter.
ACCESSION M13242 M26860
KEYWORDS M-1 killer toxin; preprotoxin; toxin.
SOURCE M-1 plasmid, cDNA to ds-RNA; and p1A1 plasmid, DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 120)
AUTHORS Hanes,S.D., Burn,V.E., Sturley,S.L., Tipper,D.J. and Bostian,K.A.
TITLE Expression of a cDNA derived from the yeast killer preprotoxin
gene: Implications for processing and immunity
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83, 1675-1679 (1986)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Sturley,S.L., Elliot,Q., LeVitre,J., Tipper,D.J. and Bostian,K.A.
TITLE Mapping of functional domains within the Saccharomyces cerevisiae
type 1 killer preprotoxin
JOURNAL EMBO J. 5, 3381-3389 (1986)
STANDARD simple staff_entry
FEATURES from to/span description
pept 1 > 120 PHO5/preprotoxin fusion peptide
matp 91 > 120 M-1 toxin, alpha subunit
recomb 52 53 PHO5/tox DNA end/BamHI linker DNA start
recomb 60 61 BamHI linker DNA end/preprotoxin DNA start
BASE COUNT 30 a 27 c 27 g 36 t
ORIGIN 52 bp upstream of BamHI site.
1 atgtttaaat ctgttgttta ttcaatttta gccgcttctt tggccaatgc aggatccgcg
61 gaaacagcac cagtctcatt actacctcgt gaagcgccgt ggtatgacaa gatctgggaa
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