GenBank-Updates@genbank.bio.net (09/22/90)
LOCUS NEUCPC 2707 bp ds-DNA PLN 22-SEP-1990
DEFINITION N.crassa cpc-1 gene, encoding cross-pathway control protein,
complete cds.
ACCESSION J03262
KEYWORDS DNA-binding protein; cpc-1 gene; transcriptional activator.
SOURCE Neurospora crassa (strain arg-12-s, cpc-1(CD15)), cDNA to mRNA and
DNA, cosmid clone 22:11:A.
ORGANISM Neurospora crassa
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Pyrenomycetes; Sordariales; Sordariaceae.
REFERENCE 1 (bases 1 to 2707)
AUTHORS Paluh,J.L., Orbach,M.J., Legerton,T.L. and Yanofsky,C.
TITLE The cross-pathway control gene of Neurospora crassa, cpc-1, encodes
a protein similar to GCN4 of yeast and the DNA-binding domain of
the oncogene v-jun-encoded protein
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 85, 3728-3732 (1988)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Paluh,J.L. and Yanofsky,C.
TITLE Characterization of Neurospora CPC1. a bZIP DNA binding protein
that does not require aligned heptad leucines for dimerization
JOURNAL Mol. Cell. Biol. (1990) In press
STANDARD full staff_entry
REFERENCE 3 (sites)
AUTHORS Ebbole,D.J., Paluh,J.L., Plamann,M.P., Sachs,M.S. and Yanofsky,C.
TITLE cpc-1, the general regulatory gene for genes of amino acid
biosynthesis in Neurospora crassa, is differentially expressed
during the asexual life cycle
JOURNAL Mol. Cell. Biol. (1990) In press
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence [1] kindly submitted by
J.L.Paluh, 29-JUL-1988.
The upstream open reading frames may play a role in translational
regulation of cpc-1.
FEATURES from to/span description
pept 1188 1197 cross-pathway control protein 1, exon 1
1255 2057 cross-pathway control protein 1, exon 2
pre-msg 464 2297 cpc-1 mRNA and intron
IVS 1198 1254 cpc-1 intron A
ORF 749 874 open reading frame 2
BASE COUNT 565 a 822 c 599 g 721 t
ORIGIN 107 bp upstream of SacI site.
1 tccgtctcca ttggctcttg gcaattcggt cagcggggct gactgcctca ggtggggcag
61 tgctagtgtg tgtaccgacc cgcaggattg gtgctttgcc cagagctcta cagaatagcg
121 cgcgcatcca tatgttagtt ctgcaatttt cttgtatcgg tgctgtgact catacttccc
181 cctttggctg gccttgcggc aaccaataag aacgcacagt gaatcttgcg ggtggggagt
241 ggatccatgg cgcctgcatt ggcttgggga cgcgcactgt cgcacacttc catctgacct
301 ttcagaaggg tttcgtggtg ggcaaggacc aaccggttgc gcggccgtgc gtgggtgcct
361 cgcccggcac tgccagggcc actgcagtgg cagtttgctg cctgatacaa aatccttccc
421 tccgcccagt tttccctctt tgaccttcct ttctcttctc tgcaaccaaa tccaccctat
481 caaaccaaaa cagtatcacg agaagcatcc atcaagatgc gttaaatcgc tcccatttcc
541 ccgttctctc gaacccaagt tcggtaatcg tcaacattaa acaattttac gttcccccct
601 cttcgtcaac cccttcagct cttccttcac aggtagatct gaacttcagc acccagcttc
661 gtagctcgcg ctcaagttct cttaccccca ccgccaccat tcacggccaa gactttccag
721 tcttcaccac ggattcccaa cagtcaacat ggcttccctc cagttcaccg agcctgccgg
781 cactctccgc gcaatcacca gcacaacaac aacagccacc acttcaggac tttgtcttgt
841 tcgatcagcc gatcagacca caccgtcaac ataaccgcaa cgctctccag cctccaaccc
901 gaggtatcaa cctgaatcag caacatcgta gccagcattt gtctccgtct ctgcagaacc
961 agcgagttgc aaacattatc caggcaacag ggcaccaact cacttcttcg gctttcacca
1021 atcggtacag ctcttctcag aactcgcgtc cgcaacagtt ctacgcttcc tcagcacctt
1081 cttcagcttc aatcctgaac actcagaacc gcgcacagca gcgccctcct gttcccttgt
1141 ttcccaaaag taccggtagt atttcgcacg gaaagcaggg caacaagatg ttctcaggta
1201 attacacctc ttatcgcatg ccgcctctca acatgcgctt acaatctcgc acagaactgg
1261 acctgctgga tttcgccacc tttgacggtg gagctaccac cgaggcggcg ttcgcttcgc
1321 ctgccaacca gacatacgat ctttcgagca gcgttcctag ctccgtctcc aacatgggca
1381 ctgtctcgcc tcaggagctt ctgctccatg agccgtatct gtctgctcct agctccactg
1441 ctcttacggc tttgacttcg ccttcgctct tcgacggctc accggatttc gataccttcg
1501 acatctctcc taattttggg cacagtgacc tggaaaaccc agacacgtgg ttctcacttt
1561 tccctgatgc caccccgcta cctcaagctc aggctcaggt ccagactcag cctcagaccc
1621 agactcagac tgagcagcaa acacaaccgc tgcctgagtt ggttcaatct gtgcagccga
1681 ccgttcagcc gaccgttgag caaacagttc actctgttga agcttcgcct gctaccccat
1741 ctgaggattt ggaggttttg tctccgggct ctggtcatca gaggaggaag tcgtctgtca
1801 gccctcccag cggtcgtcac tcgtcggtcg ctggtgttgg ctctcgccgt cgtgacaagc
1861 ctctaccccc tatcattgtt gaggatcctt ccgatgtcgt tgccatgaag cgtgctcgca
1921 acactttggc tgctcgcaag tctcgtgagc gcaaggctca gcgcttggag gagttggaag
1981 ctaagatcga ggaactgatc gcagagcgtg accgttggaa gaacttggct ttggcgcacg
2041 gtgcgtctac ggagtaaacc gaacctttcg gctccttgaa cgaaaaaaaa atatccctcg
2101 atgacgggca tcgaggaggg gtttcattgt ttttttgtga aattcttatc tctcttctct
2161 ttctggttat cattgattat tgagtgtcgc ttgatccata gatcgcgcct tgggatattt
2221 ctggtttcgg agttagatac ctttcctagc tgttacatcc agcggaaacc aaattaaaag
2281 ttttcagtgt tttttcaacc gtgttttcac aaagtgccat ctgtgagccg cttcgaggtt
2341 tgtgattttc caatgtcttc gtcgtgatgt cccttggtcc ctatgttgac acgaaccctg
2401 gcgatggaat gtctcaagac tttgttcact cgtgttcaag acttgaagcc ctaggtaaca
2461 agccttcacc atgccgtcta ttttgctgtg atgcaggaat atacaactgg ctatgtcctt
2521 gacctcgact acaaacactt gttgattgca ttgttaaaca cgctctccta tttgccctcg
2581 tcatgtgaga gtgaaggaca tgtttggtat gcgttcccgg ttgtttgaca cttcgatcta
2641 tcccttgatg actgctttcc acaccccgtc gtagcaaggc tgtcttccat cagtcatagt
2701 cggtacc
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