GenBank-Updates@genbank.bio.net (09/22/90)
LOCUS ECOAPTADK 6820 bp ds-DNA BCT 22-SEP-1990
DEFINITION E.coli sequence of the apt-adk region.
ACCESSION M38777
KEYWORDS DNA polymerase; DNA polymerase III;
adenine phosphoribosyltransferase; adenylate kinase; adk gene;
apt gene; apt protein; dnaZX gene; heat shock protein; kinase;
polymerase; recR protein; unidentified reading frame.
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Hershey,H.V. and Taylor,M.W.
TITLE Nucleotide sequence and deduced amino acid sequence of Escherichia
coli adenine phosphoribosyl-transferase and comparison with other
analogous enzymes
JOURNAL Gene 43, 287-293 (1986)
STANDARD simple automatic
REFERENCE 2 (sites)
AUTHORS Yin,K.-C., Blinkowa,A. and Walker,J.R.
TITLE Nucleotide sequence of the Escherichia coli replication gene dnaZX
JOURNAL Nucleic Acids Res. 14, 6541-6549 (1986)
STANDARD simple automatic
REFERENCE 3 (sites)
AUTHORS Flower,A.M. and McHenry,C.S.
TITLE The adjacent dnaZ and dnaX genes of Escherichia coli are contained
within one continuous open reading frame
JOURNAL Nucleic Acids Res. 14, 8091-8101 (1986)
STANDARD simple automatic
REFERENCE 4 (sites)
AUTHORS Blinkowa,A.L. and Walker,J.R.
TITLE Programmed ribosomal frameshifting generates the Escherichia coli
DNA polymerase III gamma subunit from within the tau subunit
reading frame
JOURNAL Nucleic Acids Res. 18, 1725-1729 (1990)
STANDARD simple automatic
REFERENCE 5 (sites)
AUTHORS Maki,S. and Kornberg,A.
TITLE DNA polymerase III of Escherichia coli
JOURNAL J. Biol. Chem. 263, 6547-6554 (1988)
STANDARD simple automatic
REFERENCE 6 (sites)
AUTHORS Flower,A.M. and McHenry,C.S.
TITLE The gamma subunit of DNA polymerase III holoenzyme of Escherichia
coli is produced by ribosomal frameshifting
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 3713-3717 (1990)
STANDARD simple automatic
REFERENCE 7 (sites)
AUTHORS Tsuchihashi,Z. and Kornberg,A.
TITLE Translational frameshifting generates the gamma subunit of DNA
polymerase III holoenzyme Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 2516-2520 (1990)
STANDARD simple staff_review
REFERENCE 8 (sites)
AUTHORS Yeung,T., Mullin,D.A., Chen,K.-S., Craig,E.A., Bardwell,J.C.A. and
Walker,J.R.
TITLE Sequence and expression of the Escherichia coli recR locus
JOURNAL J. Bacteriol. (1990) In press
STANDARD simple automatic
REFERENCE 9 (sites)
AUTHORS Mahdi,A.A. and Lloyd,R.G.
TITLE The recR locus of Escherichia coli K-12: molecular cloning, DNA
sequencing and identification of the gene product
JOURNAL Nucleic Acids Res. 17, 6781-6794 (1989)
STANDARD simple automatic
REFERENCE 10 (site)
AUTHORS Bardwell,J.C.A. and Craig,E.A.
TITLE Eukaryotic Mr 83,000 heat shock protein has a homologue in
Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 84, 5177-5181 (1987)
STANDARD simple automatic
REFERENCE 11 (sites)
AUTHORS Brune,M., Schumann,R. and Wittinghofer,F.
TITLE Cloning and sequencing of the adenylate kinase gene (adk) of
Escherichia coli
JOURNAL Nucleic Acids Res. 13, 7139-7151 (1985)
STANDARD simple automatic
REFERENCE 12 (sites)
AUTHORS Walker,J.E.
TITLE Distantly related sequences in the alpha- and beta-subunits of ATP
synthase, myosin, kinases and other ATP-requiring enzymes and a
common nucleotide binding fold
JOURNAL EMBO J. 1, 945-951 (1982)
STANDARD simple automatic
REFERENCE 13 (bases 1 to 6820)
AUTHORS Walker,J.R. and Peters,T.
TITLE Compilation sequence
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable copy of sequence [13] kindly
provided by J.R.Walker and T.Peters.
Author address
Univ of Texas at Austin
Dept Microbiology
University of Texas
Austin, TX 78712
FEATURES from to/span description
pept 190 741 apt ORF
pept 870 2801 dnaX ORF
pept 2854 3183 orf12 ORF
pept 3183 3788 recR ORF
pept 3898 5772 htpG ORF
pept 5953 6597 adk ORF
BASE COUNT 1683 a 1807 c 1872 g 1458 t
ORIGIN
1 gaattcggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg cagttgcaat
61 tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg gtcaaaggtt
121 aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt aacaagcagg
181 catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag catcaaaagc
241 attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt actggaagac
301 ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa tgcgggcatt
361 accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt agctctgggt
421 ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga aaccatcagt
481 gaaacttacg acctggaata cggcaccgat cagctggaga tccacgttga tgccatcaaa
541 ccgggcgaca aagttctggt ggtggacgac ctgctggcaa ccggcggcac tatcgaagcg
601 accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt cattatcaac
661 ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag ctacagcctt
721 gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta cggacagcac
781 aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc ccttcgtgaa
841 tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc cgaaaatggc
901 gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca ctggcgaacg
961 gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt ggcgtcggaa
1021 aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc attaccgcga
1081 cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt gtcgatctga
1141 ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg ctggataacg
1201 tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa gtgcatatgc
1261 tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg gagcacgtta
1321 agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg tcacgctgtc
1381 tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt gagcacatcc
1441 tcaacgaaga acatatcgct cacgagccgc gggcgctgca attgctggca cgcgccgctg
1501 aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc ggtgacggcc
1561 aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat caggcgctgt
1621 cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg attaatgaag
1681 ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc ctgttgcatc
1741 gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc gccatcgagc
1801 tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt tactatcaga
1861 cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgtcgcatg ggcgttgaga
1921 tgacgctgct gcgcgcgctg gcattccatc cgcgtatgcc gctgcctgag ccagaagtgc
1981 cacgacagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc caggtgccgc
2041 cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc
2101 aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa gcaaaaaaga
2161 gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg
2221 cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgccag
2281 ccaaaaaaga agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg
2341 tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg
2401 cgaagctagc ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt
2461 cgctaccaaa actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg
2521 cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa
2581 aactggctga agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag
2641 atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac
2701 ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggttct
2761 tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt
2821 tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac
2881 ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg
2941 cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca
3001 cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg
3061 gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa
3121 gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc
3181 tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg
3241 ttggcccgaa gtcggcgcag cgtatggcgt tcaccgtgct tcagcgcgat cgtagcggcg
3301 ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt
3361 gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa
3421 acggtcaaat ctgcgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc
3481 agttttcagg tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc
3541 cggatgatat cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag
3601 tgatcctcgc caccaacccc acggttgaag gtgaagctac cgctaactac attgccgagc
3661 tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgttccg gttggcggcg
3721 agctggaaat ggtcgacggc accacgttgt cacactccct tgccgggcgt cataagattc
3781 gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc
3841 ccatctctcc cacatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg
3901 aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc
3961 cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat
4021 gcggcggaca agctgcgttt ccgtgcgctc tctaacccgg acctgtacga aggtgatggc
4081 gaactacgcg ttcgtgtctc tttcgataaa gacaagcgta cgctgaccat ctccgataac
4141 ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt
4201 accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt
4261 cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact
4321 cgcgcggcag gcgaaaaacc agaaaatggc gtcttctggg aatcggctgg cgaaggtgaa
4381 tacaccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt
4441 gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc
4501 gaccatatcg cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt
4561 atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc
4621 accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg
4681 acctggagcc acaaccgtgt tgaaggtaag caggagtaca ccagcctgct gtacatcccg
4741 tcccaggctc cgtgggatat gtggaaccgc gatcataaac acggcctgaa actgtatgtt
4801 cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg
4861 cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac
4921 agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa
4981 aaactggcga aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta
5041 ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt
5101 tttgcttcta cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc
5161 cgcatgaaag aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg
5221 aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc
5281 gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag
5341 tcggtgtcta aagttgacga gtcgcttgaa aaactggctg acgaagttga tgagagcgcg
5401 aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag
5461 cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc
5521 gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg
5581 ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca
5641 gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg
5701 ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg
5761 ctggtttcct gatgtaatgc cggatgacct tcgtgtcatc cggcattttt cttttcatca
5821 tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gccccaggtg cctttcttga
5881 ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaattcgac acattttaag
5941 gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag
6001 gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt
6061 gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc
6121 aaactggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac
6181 tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg
6241 aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatc
6301 gttgaccgta tcgtcggtcg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa
6361 ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt
6421 aaagatgatc aggaagagac cgtacgtaaa cgtctggttg aataccatca gatgacagca
6481 ccgctgatcg gctactactc caaagaagca gaagcgggta ataccaaata cgcgaaagtt
6541 gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaattc
6601 gaaagcgcgc acggacagtc ccctcgcccc ctcgggagag ggttagggtg aggggaacag
6661 gcccgcacaa gcaaacttat cagcaatctc aggccggata ttcattcggc cttttacaaa
6721 aacctcaatc tgcgctattc ctttttctga tttgacctct cacagcaatt agttcttctt
6781 cctcactttt ccgctacaat tatcaacaag ttgaatcgat
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