GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS ECOGLYK 2028 bp ds-DNA BCT 23-SEP-1990
DEFINITION E.coli glp-kappa gene encoding glycerol kinase, complete cds.
ACCESSION M18393
KEYWORDS glycerol kinase.
SOURCE E.coli (strain K12; E.C.Lin strain 61, cell line JJ161) DNA, clone
pCJ102.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1596)
AUTHORS Pettigrew,D.W., Ma,D.-P., Conrad,C.A. and Johnson,J.R.
TITLE Escherichia coli glycerol kinase: cloning and sequencing of the
glp-kappa gene and the primary structure of the enzyme
JOURNAL J. Biol. Chem. 263, 135-139 (1988)
STANDARD full staff_entry
REFERENCE 2 (bases 1546 to 2028)
AUTHORS Pettigrew,D.W.
JOURNAL Unpublished (1988) Dept. Biochem. & Biophys., Texas A&M Univ, Texas
STANDARD full staff_entry
COMMENT Draft and computer_readable sequence of [1] kindly provided by
Pettigrew,D.W. 18-FEB-1988.
FEATURES from to/span description
pept 37 1545 ATP:glycerol 3-phosphotransferase (EC
2.1.7.30) /nomgen="glpK"
site 1740 2028 putative VECTOR sequence lambda
BASE COUNT 513 a 507 c 558 g 450 t
ORIGIN 467 bp upstream of BamHI site.
1 aagcttcgct gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa atatatcgtt
61 gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc
121 attagcgtgt cgcagcgcga atttgagcaa atctacccaa aaccaggttg ggtagaacac
181 gacccaatgg aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc
241 gatatcagtt ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt
301 gtctgggaaa aagaaaccgg caagcctatc tataacgcca ttgtctggca gtgccgtcgt
361 accgcagaaa tctgcgagca tttaaaacgt gacggtttag aagattatat ccgcagcaat
421 accggtctgg tgattgaccc gtacttttct ggcaccaaag tgaagtggat cctcgaccat
481 gtggaaggct ctcgcgagcg tgcacgtcgt ggtgaattgc tgtttggtac ggttgatacg
541 tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct
601 cgtaccatgt tgttcaacat ccataccctg gactgggacg acaaaatgct ggaagtgctg
661 gatattccgc gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact
721 aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag
781 gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact
841 ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg
901 accaccatcg cctgcggccc gactggcgaa gtgaactatg cgttggaagg tgcggtgttt
961 atggcaggcg catccattca gtggctgcgc gatgaaatga agttgattaa cgacgcctac
1021 gattccgaat atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt ggttccggca
1081 tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat tttcggtctg
1141 actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag
1201 acgcgtgacg tgctggaagc gatgcaggcc gactctggta tccgtctgca cgccctgcgc
1261 gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt tccagtccga tattctcggc
1321 acccgcgttg agcgcccgga agtgcgcgaa gtcaccgcat tgggtgcggc ctatctcgca
1381 ggcctggcgg ttggcttctg gcagaacctc gacgagctgc aagagaaagc ggtgattgag
1441 cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa
1501 aaagcggtta aacgcgcgat ggcgtgggaa gaacacgacg aataatgtaa atgccgaatg
1561 aagcgtttat gccgcatccg gtagtcccga aacgtgcggg ggcaaccccg cacacatcaa
1621 taatccctcc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
1681 tgagacgaga acttgccatc gaattttccc gcgtcaccga atacgcggcg ctggctggct
1741 acaattggtt atgtgttttg ggggcgatcg tgaggcaaat actacacgct gaggccgggt
1801 tattcttgtt ctctggtcaa attatatagt tggaaaacaa ggatgcatat atgaatgaac
1861 gatgcagagg caatcgcgat ggcgatagtg ggtatcatgt agcccgctta tgctggaaag
1921 aagcaataac ccgcagaaaa acaaagctcc aagctcaaca aaactaaggg catagacaat
1981 aactaccgat gtcatatcac catactctct aatcttgcgc agtcggcg
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