GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS ECORGNB 7508 bp ds-DNA BCT 23-SEP-1990
DEFINITION E.coli rRNA operon (rrnB) coding for Glu-tRNA-2, 5S, 16S and 23S
rRNA.
ACCESSION J01695
KEYWORDS 16S ribosomal RNA; 23S ribosomal RNA; 5S ribosomal RNA; promoter;
ribosomal RNA; rrnb operon; transfer RNA; transfer RNA-Glu.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1518 to 3059)
AUTHORS Brosius,J., Palmer,M.L., Kennedy,P.J. and Noller,H.F.
TITLE Complete nucleotide sequence of a 16S ribosomal RNA gene from
Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 75, 4801-4805 (1978)
STANDARD full staff_review
REFERENCE 2 (bases 811 to 1543; prm region)
AUTHORS Csordas-Toth,E., Boros,I. and Venetianer,P.
TITLE Structure of the promoter region for the rrnB gene in Escherichia
coli
JOURNAL Nucleic Acids Res. 7, 2189-2197 (1979)
STANDARD full staff_review
REFERENCE 3 (bases 3500 to 6403)
AUTHORS Brosius,J., Dull,T.J. and Noller,H.F.
TITLE Complete nucleotide sequence of a 23S ribosomal RNA gene from
Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77, 201-204 (1980)
STANDARD full staff_review
REFERENCE 4 (bases 1 to 7508)
AUTHORS Brosius,J., Dull,T.J., Sleeter,D.D. and Noller,H.F.
TITLE Gene organization and primary structure of a ribosomal RNA operon
from Escherichia coli
JOURNAL J. Mol. Biol. 148, 107-127 (1981)
STANDARD full staff_review
REFERENCE 5 (bases 6412 to 6507; 6603 to 6657)
AUTHORS Singh,B. and Apirion,D.
TITLE Primary and secondary structure in a precursor of 5S rRNA
JOURNAL Biochim. Biophys. Acta 698, 252-259 (1982)
STANDARD full staff_review
REFERENCE 6 (bases 6518 to 6931)
AUTHORS Sarmientos,P., Sylvester,J.E., Contente,S. and Cashel,M.
TITLE Differential stringent control of the tandem E.coli ribosomal RNA
promoters from the rRNA operon expressed in vivo in multicopy
plasmids
JOURNAL Cell 32, 1337-1346 (1983)
STANDARD full staff_review
REFERENCE 7 (bases 3380 to 3509; 6392 to 6503)
AUTHORS King,T.C., Sirdeshmukh,R. and Schlessinger,D.
TITLE RNase III cleavage is obligate for maturation but not for function
of Escherichia coli pre-23S rRNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81, 185-188 (1984)
STANDARD full staff_review
COMMENT Tandem promoters direct rRNA synthesis.
[5] found that RNase E cleaves the 5S rRNA from a 9S precursor RNA
by cutting the 9S RNA bonds between positions 6492-6493 and
6619-6620.
[6] sequenced a recombinant of the 5' end of the rRNA operon and
the 3' end of the rrnB operon.
In RNase III- strains, the 16S rRNA is produced directly without
the formation of intermediates by RNase III [7]. Mature 23S rRNA
is not formed, but several unmatured 23S rRNA species are produced.
At least some of these novel 23S species must form ribosomes active
in protein synthesis, because although no normal termini are
present, the strain is viable. The removal of the extra sequences
is then not absolutely required for the function of at least 23S
rRNA, but may represent a later evolutionary development that
further improves ribosome activity [7].
EMBL features not translated to GenBank features:
key from to description
SITE 240 254 lambda attachment site
CDS 275 1144 unidentified reading frame I
FEATURES from to/span description
rRNA 1518 3059 16S rRNA (rrnB)
rRNA 3500 6403 23S rRNA (rrnB)
rRNA 6496 6615 5S rRNA (rrnB)
tRNA 3231 3306 Glu-tRNA-2 (rrnB)
RNA 1223 > 3306 rrnB precursor RNA (alt.)
RNA 1344 > 3306 rrnB precursor RNA (alt.)
conflict 1068 1072 cagga in [4]; ca in [2]
signal 1212 1218 promoter: RNA polymerase binding site
signal 1331 1337 alternate promoter
conflict 1507 1507 g in [4]; a in [2]
conflict 1605 1606 tt in [4]; t in [2]
anticdn 3265 3267 Glu-tRNA-2 anticodon ttc
site 1 250 putative VECTOR sequence lambda
BASE COUNT 1969 a 1694 c 2166 g 1679 t
ORIGIN 1 bp upstream from BamHI site.
1 ggatccgcct acctttcacg agttgcgcag tttgtctgca agactctatg agaagcagat
61 aagcgataag tttgctcaac atcttctcgg gcataagtcg gacaccatgg catcacagta
121 tcgtgatgac agaggcaggg agtgggacaa aattgaaatc aaataatgat tttattttga
181 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact
241 tagtataaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact
301 gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct
361 ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc
421 ggatctccat tacatttatg ctttcgataa cgtcgctttc ccgtatggcg aaaaaagcga
481 agcgtttatt gttgagcgag tggtggcaat tgtcaccgcg gtgcaagaac gttatcccct
541 tgcgctggct gtggtcgctt gcaacactgc cagtaccgtt tcacttcctg cattacgcga
601 aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac
661 ggcaaatggc attgtcggat tactggcaac ccgcggaaca gttaaacgtt cttatactca
721 tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat
781 ggttgagttg gctgaagcga agctacatgg cgaagatgtt tctctggatg cactaaaacg
841 tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac
901 ccatttccct ctactacaag aagaactgtt acaagtgctg ccagagggaa cccggctggt
961 ggattctggc gcagcgattg ctcgccgaac ggcctggttg ttagaacatg aagccccgga
1021 tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag gagctgaaca
1081 attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg
1141 ctgatttggt tgaatgttgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
1201 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
1261 cagcggggtt ctcctgagaa ctccggcaga gaaagcaaaa ataaatgctt gactctgtag
1321 cgggaaggcg tattatgcac accccgcgcc gctgagaaaa agcgaagcgg cactgctctt
1381 taacaattta tcagacaatc tgtgtgggca ctcgaagata cggattctta acgtcgcaag
1441 acgaaaaatg aataccaagt ctcaagagtg aacacgtaat tcattacgaa gtttaattct
1501 ttgagcgtca aacttttaaa ttgaagagtt tgatcatggc tcagattgaa cgctggcggc
1561 aggcctaaca catgcaagtc gaacggtaac aggaagaagc ttgcttcttt gctgacgagt
1621 ggcggacggg tgagtaatgt ctgggaaact gcctgatgga gggggataac tactggaaac
1681 ggtagctaat accgcataac gtcgcaagac caaagagggg gaccttcggg cctcttgcca
1741 tcggatgtgc ccagatggga ttagctagta ggtggggtaa cggctcacct aggcgacgat
1801 ccctagctgg tctgagagga tgaccagcca cactggaact gagacacggt ccagactcct
1861 acgggaggca gcagtgggga atattgcaca atgggcgcaa gcctgatgca gccatgccgc
1921 gtgtatgaag aaggccttcg ggttgtaaag tactttcagc ggggaggaag ggagtaaagt
1981 taataccttt gctcattgac gttacccgca gaagaagcac cggctaactc cgtgccagca
2041 gccgcggtaa tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca
2101 ggcggtttgt taagtcagat gtgaaatccc cgggctcaac ctgggaactg catctgatac
2161 tggcaagctt gagtctcgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag
2221 agatctggag gaataccggt ggcgaaggcg gccccctgga cgaagactga cgctcaggtg
2281 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc
2341 gacttggagg ttgtgccctt gaggcgtggc ttccggagct aacgcgttaa gtcgaccgcc
2401 tggggagtac ggccgcaagg ttaaaactca aatgaattga cgggggcccg cacaagcggt
2461 ggagcatgtg gtttaattcg atgcaacgcg aagaacctta cctggtcttg acatccacgg
2521 aagttttcag agatgagaat gtgccttcgg gaaccgtgag acaggtgctg catggctgtc
2581 gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac gagcgcaacc cttatccttt
2641 gttgccagcg gtccggccgg gaactcaaag gagactgcca gtgataaact ggaggaaggt
2701 ggggatgacg tcaagtcatc atggccctta cgaccagggc tacacacgtg ctacaatggc
2761 gcatacaaag agaagcgacc tcgcgagagc aagcggacct cataaagtgc gtcgtagtcc
2821 ggattggagt ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg tggatcagaa
2881 tgccacggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca tgggagtggg
2941 ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct taccactttg tgattcatga
3001 ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg cggttggatc acctccttac
3061 cttaaagaag cgtactttgt agtgctcaca cagattgtct gatagaaagt gaaaagcaag
3121 gcgtttacgc gttgggagtg aggctgaaga gaataaggcc gttcgctttc tattaatgaa
3181 agctcaccct acacgaaaat atcacgcaac gcgtgataag caattttcgt gtccccttcg
3241 tctagaggcc caggacaccg ccctttcacg gcggtaacag gggttcgaat cccctagggg
3301 acgccacttg ctggtttgtg agtgaaagtc gccgacctta atatctcaaa actcatcttc
3361 gggtgatgtt tgagattttt gctctttaaa aatctggatc aagctgaaaa ttgaaacact
3421 gaacaacgag agttgttcgt gagtctctca aattttcgca acacgatgat gaatcgaaag
3481 aaacatcttc gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc
3541 agaggcgatg aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta
3601 taaccggcga tttccgaatg gggaaaccca gtgtgtttcg acacactatc attaactgaa
3661 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
3721 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
3781 agtgtgtgtg ttagtggaag cgtctggaaa ggcgcgcgat acagggtgac agccccgtac
3841 acaaaaatgc acatgctgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
3901 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
3961 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
4021 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
4081 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
4141 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
4201 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
4261 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
4321 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
4381 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
4441 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
4501 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
4561 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
4621 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
4681 gttgggtagg ggagcgttct gtaagcctgc gaaggtgtgc tgtgaggcat gctggaggta
4741 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
4801 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
4861 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
4921 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
4981 ggttttccag gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc
5041 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
5101 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
5161 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg
5221 aggtccctcg cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
5281 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
5341 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
5401 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
5461 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
5521 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
5581 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
5641 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
5701 ttgacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
5761 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
5821 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
5881 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
5941 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
6001 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
6061 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
6121 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
6181 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
6241 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
6301 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
6361 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagctgtt
6421 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
6481 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
6541 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
6601 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
6661 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
6721 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
6781 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
6841 cctgtcgtca tatctacaag ccatcccccc acagatacgg taaactagcc tcgtttttgc
6901 atcaggaaag cagctatgaa ccactcctta aaaccctgga acacatttgg cattgatcat
6961 aatgctcagc acattgtatg tgccgaagac gaacaacaat tactcaatgc ctggcagtat
7021 gcaaccgcag aaggacaacc cgttcttatt ctgggtgaag gaagtaatgt actttttctg
7081 gaggactatc gcggcacggt gatcatcaac cggatcaaag gtatcgaaat tcatgatgaa
7141 cctgatgcgt ggtatttaca tgtaggagcc ggagaaaact ggcatcgtct ggtaaaatac
7201 actttgcagg aaggtatgcc tggtctggaa aatctggcat taattcctgg ttgtgtcggc
7261 tcatcaccta tccagaatat tggtgcttat ggcgtagaat tacagcgagt ttgcgcttat
7321 gttgattctg ttgaactggc gacaggcaag caagtgcgct taactgccaa agagtgccgt
7381 tttggctatc gcgacagtat ttttaaacat gaataccagg accgcttcgc tattgtagcc
7441 gtaggtctgc gtctgccaaa agagtggcaa cctgtactaa cgtatggtga cttaactcgt
7501 ctggatcc
//