GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS KPNAACA4 1352 bp ds-DNA BCT 23-SEP-1990 DEFINITION Plasmid pJHC-MW1 aminoglycoside 6'-N-acetyltransferase (aacA4) gene, complete cds, and streptomycin-spectinomycin resistance (aadA) gene, 5' end. ACCESSION M21682 KEYWORDS ORF; acetyltransferase; amikacin resistance gene; aminoglycoside 6'-N-acetyltransferase; streptomycin-spectinomycin resistance gene; transposon. SOURCE Plasmid pJHC-MW1 DNA, isolated from K.pneumoniae, clone pJHC-T15.2. ORGANISM Plasmid pJHC-MW1 Prokaryota; Bacteria. REFERENCE 1 (bases 1 to 1352) AUTHORS Nobuta,K., Tolmasky,M.E., Crosa,L.M. and Crosa,J.H. TITLE Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae JOURNAL J. Bacteriol. 170, 3769-3773 (1988) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence [1] kindly submitted by J.H.Crosa, 03-JAN-1989. FEATURES from to/span description pept 380 985 aminoglycoside 6'-N-acetyltransferase pept 1053 > 1352 streptomycin-spectinomycin resistance ORF 488 985 ORF 2 ORF 994 749 (c) ORF 3 site < 1 > 1352 Tn1331 transposon site 172 398 putative VECTOR sequence pBR322 BASE COUNT 358 a 322 c 374 g 298 t ORIGIN 1 bp upstream of BamHI site. 1 ggatcctaga gcgcacgaat gagggccgac aggaagcaaa gctgaaagga atcaaatttg 61 gccgcaggcg taccgtggac aggaacgtcg tgctgacgct tcatcagaag ggcactggtg 121 caacggaaat tgctcatcag ctcagtattg cccgctccac ggtttataaa attcttgaag 181 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 241 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 361 atattgaaaa aggaagagta tgagtattca acatttccaa acaaagttag gcatcacaaa 421 gtacagcatc gtgaccaaca gcaacgattc cgtcacactg cgcctcatga ctgagcatga 481 ccttgcgatg ctctatgagt ggctaaatcg atctcatatc gtcgagtggt ggggcggaga 541 agaagcacgc ccgacacttg ctgacgtaca ggaacagtac ttgccaagcg ttttagcgca 601 agagtccgtc actccataca ttgcaatgct gaatggagag ccgattgggt atgcccagtc 661 gtacgttgct cttggaagcg gggacggatg gtgggaagaa gaaaccgatc caggagtacg 721 cggaatagac cagttactgg cgaatgcatc acaactgggc aaaggcttgg gaaccaagct 781 ggttcgagct ctggttgagt tgctgttcaa tgatcccgag gtcaccaaga tccaaacgga 841 cccgtcgccg agcaacttgc gagcgatccg atgctacgag aaagcggggt ttgagaggca 901 aggtaccgta accaccccag atggtccagc cgtgtacatg gttcaaacac gccaggcatt 961 cgagcgaaca cgcagtgttg cctaaccctt ccatcgaggg ggacgtccaa gggctggcgc 1021 cttggccgcc ctcatgtcaa acgttaaaca tcatgaggga agcggtgatc gcgaaagtat 1081 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 1141 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 1201 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 1261 ttttggaaac ttcggcttcc cctggagaga gcgggggcaa atggtggtca ccatcctgtc 1321 ggctgtggca caggctgaac gccggaggat cc //