GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS KPNAACA4 1352 bp ds-DNA BCT 23-SEP-1990
DEFINITION Plasmid pJHC-MW1 aminoglycoside 6'-N-acetyltransferase (aacA4)
gene, complete cds, and streptomycin-spectinomycin resistance
(aadA) gene, 5' end.
ACCESSION M21682
KEYWORDS ORF; acetyltransferase; amikacin resistance gene;
aminoglycoside 6'-N-acetyltransferase;
streptomycin-spectinomycin resistance gene; transposon.
SOURCE Plasmid pJHC-MW1 DNA, isolated from K.pneumoniae, clone pJHC-T15.2.
ORGANISM Plasmid pJHC-MW1
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1352)
AUTHORS Nobuta,K., Tolmasky,M.E., Crosa,L.M. and Crosa,J.H.
TITLE Sequencing and expression of the 6'-N-acetyltransferase gene of
transposon Tn1331 from Klebsiella pneumoniae
JOURNAL J. Bacteriol. 170, 3769-3773 (1988)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence [1] kindly submitted by
J.H.Crosa, 03-JAN-1989.
FEATURES from to/span description
pept 380 985 aminoglycoside 6'-N-acetyltransferase
pept 1053 > 1352 streptomycin-spectinomycin resistance
ORF 488 985 ORF 2
ORF 994 749 (c) ORF 3
site < 1 > 1352 Tn1331 transposon
site 172 398 putative VECTOR sequence pBR322
BASE COUNT 358 a 322 c 374 g 298 t
ORIGIN 1 bp upstream of BamHI site.
1 ggatcctaga gcgcacgaat gagggccgac aggaagcaaa gctgaaagga atcaaatttg
61 gccgcaggcg taccgtggac aggaacgtcg tgctgacgct tcatcagaag ggcactggtg
121 caacggaaat tgctcatcag ctcagtattg cccgctccac ggtttataaa attcttgaag
181 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
241 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
361 atattgaaaa aggaagagta tgagtattca acatttccaa acaaagttag gcatcacaaa
421 gtacagcatc gtgaccaaca gcaacgattc cgtcacactg cgcctcatga ctgagcatga
481 ccttgcgatg ctctatgagt ggctaaatcg atctcatatc gtcgagtggt ggggcggaga
541 agaagcacgc ccgacacttg ctgacgtaca ggaacagtac ttgccaagcg ttttagcgca
601 agagtccgtc actccataca ttgcaatgct gaatggagag ccgattgggt atgcccagtc
661 gtacgttgct cttggaagcg gggacggatg gtgggaagaa gaaaccgatc caggagtacg
721 cggaatagac cagttactgg cgaatgcatc acaactgggc aaaggcttgg gaaccaagct
781 ggttcgagct ctggttgagt tgctgttcaa tgatcccgag gtcaccaaga tccaaacgga
841 cccgtcgccg agcaacttgc gagcgatccg atgctacgag aaagcggggt ttgagaggca
901 aggtaccgta accaccccag atggtccagc cgtgtacatg gttcaaacac gccaggcatt
961 cgagcgaaca cgcagtgttg cctaaccctt ccatcgaggg ggacgtccaa gggctggcgc
1021 cttggccgcc ctcatgtcaa acgttaaaca tcatgaggga agcggtgatc gcgaaagtat
1081 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
1141 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
1201 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
1261 ttttggaaac ttcggcttcc cctggagaga gcgggggcaa atggtggtca ccatcctgtc
1321 ggctgtggca caggctgaac gccggaggat cc
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