GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS SLMRGI 979 bp ds-DNA PLN 23-SEP-1990
DEFINITION Slime mold (P.polycephalum) rRNA gene, 5' end and flank
(transcription initiation sequence).
ACCESSION K01607
KEYWORDS ribosomal RNA; transcription initiation.
SOURCE Slime mold (P. polycephalum, strain a x i), DNA.
ORGANISM Physarum polycephalum
Eukaryota; Plantae; Thallobionta; Myxomycota; Myxomycetes;
Myxogastromycetidae; Physarales; Physaraceae.
REFERENCE 1 (bases 1 to 979)
AUTHORS Johnson,E.M.
JOURNAL Unpublished (1984) Rockefeller U., New York, NY 10021.
STANDARD simple staff_review
COMMENT Submitted by the author, who has submitted it for publication with
the following abstract:
In cloned rDNA segments of Physarum polycephalum, strain a x i
sequences sufficient for proper transcription initiation in vitro
are located within -93 to +85 nucleotides of the start site. A
sequence of 978 nucleotides including this region is presented. A
comparison of initiation-promoter region sequences from strains a x
i and M3CVIII (B. Blum et al., Nucleic Acids Res. 11, 8519-8531)
reveals blocks of homology separated by intervening unshared
sequences. The sequence at the initiation site (-12 to +2) is
nearly identical in these strains and is flanked by unshared
regions. A sequence pattern resembling this segment is found
throughout rDNA of eukaryotes within the region -12 to +4. An
aspect of this pattern, most strongly represented in lower
eukaryotes, consists of alternating A residues in the form (AX)n,
where n = 2-8. Of 15 compared initiation sequences, 100% possess a
TA pair within 5 nucleotides of the start site in a relatively
conserved 4-nucleotide tract. The sequence presented begins at -93
as the first nucleotide, where +1 is the transcription initiation
site.
FEATURES from to/span description
RNA 94 > 94 precursor RNA
site 181 248 putative VECTOR sequence lambda
BASE COUNT 227 a 256 c 266 g 230 t
ORIGIN
1 ctcgacatag acaatgcgta cctcaacgga gacatagata cagacctgta catgatccac
61 ctcaaggtgg acatgggacg acggatacag tagagaacag ggatacgtgc tcaaggctga
121 acaagagcct ctacggcatc aagcaagcag gacacatttg gaattccaca atccacgcct
181 actgtgtccg gaaaccgcgg acaactggta tcccagtggc ctaacgaaca gttcaccgtt
241 aaaggcgttt ttccacaggc atgttgctct gccgggctaa ccccggtggg acaaaaacct
301 tgttaatcca agccataaac actcgtatac ccctaaaagg gacccgatgt ggaggtgggg
361 gggagacgcc acttaacggc ggtgttctcc tttcctatcg cccttcgccg ccctatatta
421 ctgttggccg ctgtgcaacc ccggggctct cttaggccaa ttagtcactt ggcccaagag
481 tacgcgcagt taacagttgg gttggctagg tccgagagtc tcaattaagt gtgttagctc
541 actagttagg tcagcccagg gtgcttactc gtagtccttc cggctcgtag tatgtgtgga
601 agattagata taatttaagg tggatggagg cgtatggaac tcgttcgtgt ggctatgcac
661 catccggggc gcttcgaacc gcattgtctg ctcggacaaa acaatgacgg aacccctttc
721 tgtcggcaac cgtgaagcaa gcgcgtatct cttctttctt ttccccgttg actttaaccg
781 gttccggcgt ggttggggga ggtggggtac cgcttggatt ctgtcggatc gcgaaacacg
841 ttctcaaaac accagtgtag agacctctct cctccgtgcg aatcagttgg gcacaacgca
901 gattccgatc ctgtagtgag cgtctggcgg gtaaacgtgg tttcgtgaca ttcggccatt
961 cgaacggccg ggagtcgac
//