GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS SOYHSP175 2484 bp ds-DNA PLN 23-SEP-1990
DEFINITION Soybean (Glycine max) low MW heat shock protein gene (Gmhsp17.5-M).
ACCESSION M11318
KEYWORDS heat shock protein.
SOURCE Soybean (Glycine max var. Corsoy) DNA, clone pM/EE2.4.
ORGANISM Glycine max
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 4 to 2484)
AUTHORS Nagao,R.T., Czarnecka,E., Gurley,W.B., Schoeffl,F. and Key,J.L.
TITLE Genes for low-molecular-weight heat shock proteins of soybeans:
Sequence analysis of a multigene family
JOURNAL Mol. Cell. Biol. 5, 3417-3428 (1985)
STANDARD simple staff_review
REFERENCE 2 (bases 1 to 2472)
AUTHORS Nagao,R.T.
JOURNAL Unpublished (1986) Botany Dept., Univ. of Georgia, Athens GA 30602
STANDARD simple staff_review
COMMENT Draft entry and sequence for [1] in computer readable form kindly
provided by R.T.Nagao, 18-FEB-1986.
The heat shock proteins are encoded by large multigene families.
There are about thirteen small heat shock proteins in the family
described in [1]. They are found at locus 67B and are closely
linked, suggesting that they have a common origin and arose by gene
duplication and inversion events. There also are multiple cross-
homologies in the 5' flanking regions of these genes.
Potential heat shock consensus elements are located at positions
363-377, 804-818 and 814-828.
FEATURES from to/span description
pept 964 1425 17.5 kd heat shock protein Gmhsp17.6L
mRNA 871 1605 HSP mRNA (alt.)
mRNA 871 1615 HSP mRNA (alt.)
mRNA 871 1741 HSP mRNA (alt.)
mRNA 876 1605 HSP mRNA (alt.)
mRNA 876 1615 HSP mRNA (alt.)
mRNA 876 1741 HSP mRNA (alt.)
site 2406 2484 putative VECTOR sequence M13mp19
BASE COUNT 791 a 442 c 435 g 816 t
ORIGIN 6 bp upstream of EcoRI site.
1 cccgggaatt ctacaattgg gactatatcg gattccttag cctttttttt gtatttgttt
61 ggttgtatta tactgtgacc aaaataatgt ttatgaggct attgctatat ccattcctct
121 tgttgctaag tacaccctta ccttttttct aaacaaatac ttaaataacc ttttcccttc
181 aaaccttctc tcttgcgcac tcccttttcc cccacaacaa caaacttctc tttttcatag
241 caacaacaca tttttttctc tttcatcaca ttctcacctc tcttttcatt cccttcaatc
301 tcatgctctg gtctcccttc tccccttgtt tagtctcttg ctttagtgtt aggatttttt
361 catcttcaaa cttcaagttg ttgtagtgga ccaagtcaaa ttgctgagat tgaaggacta
421 atacaaaata tatgtttttt ccacttcagt tcctccacca tagtcttagt attctgcttg
481 aatttgtttt agatattttt cttaacatcc caaaaattac tttctgaaaa gaaaaacaat
541 attctagaaa aatattttga ataacacatt ttttttaata ttctgaaaaa tatttttcag
601 aacacaacaa tatttcagaa tttataggta caaagatttt aataaaaaag gatggtgaat
661 atagcaaaag cctatttatg aacgatatca accagaacta gaacaagaaa aataaatgca
721 ctagaacctt cgtacacgga gtggagaagt ccagaagttt ttatagaatc atttgaaact
781 ggtaaaacca accaaattgc aaacacgatt tttctggaac gtacacgatt atcctttcac
841 ttactttaaa tacatcacga ttagtcagaa aaacgaaacg aagaaaagag ttacaaagtt
901 acctgtatac gatctcattt tgatctccca agtttcaaat ctcgcgaata aatatatcaa
961 aagatgtctc tgattccaag tattttcggt ggcagaagga gcaacgtgtt cgatcctttc
1021 tccctcgacg tgtgggatcc cttcaaggat tttcattttc ccacttctct ttctgctgaa
1081 aattctgcat ttgtgaacac tcgtgtggat tggaaagaaa ccccagaagc acacgtgttc
1141 gaggctgata ttccagggct gaagaaggag gaagtgaagg tgcagattga agatgatagg
1201 gttcttcaga taagcggaga gaggaacctt gagaaggaag acaagaacga cacgtggcat
1261 cgcgtggagc gtagcagcgg caacttcatg aggaggttca ggttgccgga gaatgctaaa
1321 gtggagcaag tgaaggcttc tatggaaaat ggtgttctca cagttaccgt tcccaaagaa
1381 gaggttaaga agcctgatgt taaggccatt gaaatctctg gttaaacttg ttgttgctta
1441 aaatcgtgag ctttgctttg tgtaataact aataagtatt ctctgtcatg tgttcctgtg
1501 gtgaattttg agaattgaaa gattttacat tgtgaaatgt aactgcttaa gtggtaattg
1561 tgtgcttgtg taattcatgt gaataaagtc cagccttcag aaaatactat aatttgctat
1621 atcacaagtg tctgatatgt gatgtagaca aaaaaataga ttgtgtaatg tgaattgtaa
1681 ttggcctata gcgtgcattt gttatagccg agagactttc tctctatgtt cataaactcc
1741 acctcccagt aagtgtataa tattccatct taataatgaa aataaataga cgatttagtg
1801 tgttcccatg cataactaaa gactatagag gatttgatat tctcttccaa gggcaagagt
1861 aaattgaaaa taagactaga accaactttt ttgtcttgat ttttaataaa caaatgtaca
1921 accgaggata tatagactgt ggtcatcaac agcataaaat cacttagaag ttggaatagc
1981 tagcttgcat tatttaggtt gtaaagaccc tgcaataacc ttgaaaattt tgaggctttg
2041 gatactcatc tgtttgatta attatctaaa ttttaagact tatatttatt tatacgcaag
2101 taggtgtgga aatgagtcaa gttaagtcaa gctttattag gcttgagctc ggcttgagtt
2161 gaatacgtaa ggcttgggtt tgacttaatt aacctgatct aattagactt ttttaaagga
2221 tctctagatc tcgatcttac ataaaagtct ggcttgaccc acgagcctat ttaaaagctt
2281 gcttaaagac gtctttgatt aattaattat tttaaaacct agtgaaatac taactaaaaa
2341 aagaaactta taaatttcat gtaaataatg tacaaatcca aaaataattg ataaacaaaa
2401 tcatattgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
2461 cccaacttaa tcgccttgca gcac
//