GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS STALYSA 1825 bp ds-DNA BCT 23-SEP-1990
DEFINITION Staphylococcus staphylolyticus lysostaphin gene.
ACCESSION X06121
KEYWORDS inverted repeat; lysostaphin.
SOURCE Staphylococcus staphylolyticus.
ORGANISM Staphylococcus staphylolyticus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 1825)
AUTHORS Heinrich,P., Rosenstein,R., Boehmer,M., Sonner,P. and Goetz,F.
TITLE The molecular organization of the lysostaphin gene and its
sequences repeated in tandem
JOURNAL Mol. Gen. Genet. 209, 563-569 (1987)
STANDARD simple automatic
COMMENT Data kindly reviewed (02-SEP-1988) by Goetz F.
EMBL features not translated to GenBank features:
key from to description
RBS 206 212 Shine-Dalgarno sequence
INVREP 1663 1665 inverted repeat A
INVREP 1666 1668 inverted repeat A'
INVREP 1694 1700 inverted repeat B
INVREP 1709 1715 inverted repeat B'
INVREP 1760 1771 imp.inverted repeat C
INVREP 1782 1793 imp.inverted repeat C'
FEATURES from to/span description
pept 220 1662 lysostaphin (ttg start)
site 1729 1824 putative VECTOR sequence M13mp19
BASE COUNT 699 a 302 c 345 g 479 t
ORIGIN 345 bp upstream of FokI site.
1 gaaaattcca aaaaaaaacc tactttctta atattgattc atattatttt aacacaatca
61 gttagaattt caaaaatctt aaagtcaatt tttgagtgtg tttgtatatt tcatcaaagc
121 caatcaatat tattttactt tcttcatcgt taaaaaatgt aatatttata aaaatatgct
181 attctcataa atgtaataat aaattaggag gtattaaggt tgaagaaaac aaaaaacaat
241 tattatacga cacctttagc tattggactg agtacatttg ccttagcatc tattgtttat
301 ggagggattc aaaatgaaac acatgcttct gaaaaaagta atatggatgt ttcaaaaaaa
361 gtagctgaag tagagacttc aaaaccccca gtagaaaata cagctgaagt agagacttca
421 aaagctccag tagaaaatac agctgaagta gagacttcaa aagctccagt agaaaataca
481 gctgaagtag agacttcaaa agctccagta gaaaatacag ctgaagtaga gacttcaaaa
541 gctccggtag aaaatacagc tgaagtagag acttcaaaag ctccggtaga aaatacagct
601 gaagtagaga cttcaaaagc cccagtagaa aatacagctg aagtagagac ttcaaaagct
661 ccagtagaaa atacagctga agtagagact tcaaaagctc cggtagaaaa tacagctgaa
721 gtagagactt caaaagcccc agtagaaaat acagctgaag tagagacttc aaaagctcca
781 gtagaaaata cagctgaagt agagacttca aaagctccgg tagaaaatac agctgaagta
841 gagacttcaa aagccccagt agaaaataca gctgaagtag agacttcaaa agccctggtt
901 caaaatagaa cagctttaag agctgcaaca catgaacatt cagcacaatg gttgaataat
961 tacaaaaaag gatatggtta cggtccttat ccattaggta taaatggcgg tatccactac
1021 ggagttgatt tttttatgaa tattggaaca ccagtaaaag ctatttcaag cggaaaaata
1081 gttgaagctg gttggagtaa ttacggagga ggtaatcaaa taggtcttat tgaaaatgat
1141 ggagtgcata gacaatggta tatgcatcta agtaaatata atgttaaagt aggagattat
1201 gtcaaagctg gtcaaataat cggttggtct ggaagcactg gttattctac agcaccacat
1261 ttacacttcc aaagaatggt taattcattt tcaaattcaa ctgcccaaga tccaatgcct
1321 ttcttaaaga gcgcaggata tggaaaagca ggtggtacag taactccaac gcccaataca
1381 ggttggaaaa caaacaaata tggcacacta tataaatcag agtcagctag cttcacacct
1441 aatacagata taataacaag aacgactggt ccatttagaa gcatgccgca gtcaggagtc
1501 ttaaaagcag gtcaaacaat tcattatgat gaagtgatga aacaagacgg tcatgtttgg
1561 gtaggttata caggtaacag tggccaacgt atttacttgc ctgtaagaac atggaataaa
1621 tctactaata ctttaggtgt tctttgggga actataaagt gagcgcgctt tttataaact
1681 tatatgataa ttagagcaaa taaaaatttt ttctcattcc taaagttgaa gcttttcgta
1741 atcatgtcat agcgtttcct gtgtgaaatt gcttagcctc acaattccac acaacatacg
1801 agccggaaca taaagtgcta agcct
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