GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS STRPBGSL 3217 bp ds-DNA BCT 23-SEP-1990
DEFINITION S.lactis phospho-beta-D-galactosidase gene, complete cds.
ACCESSION M19454
KEYWORDS D-galactose-6-phosphate; D-glucose; phospho-beta-D-galactosidase.
SOURCE S.lactis (strain Z268) DNA, clone X25.
ORGANISM Streptococcus lactis
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Streptococcaceae.
REFERENCE 1 (bases 401 to 2100)
AUTHORS Boizet,B., Villeval,D., Slos,P., Novel,M., Novel,G. and
Mercenier,A.
TITLE Isolation and structural analysis of the phospho-beta-
galactosidase gene from Streptococcus lactis Z268
JOURNAL Gene 62, 249-261 (1988)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 3217)
AUTHORS Mercenier,A.
JOURNAL Unpublished (1988) Transgene S.A., Stasbourg Cedex France
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly provided
by A.Mercenier, 13-JUN-1988
FEATURES from to/span description
pept 601 2034 phospho-beta-D-galactosidase (EC 3.2.1.85)
site 2953 3143 putative VECTOR sequence pBR322
site 3027 3217 putative VECTOR sequence pBR322
BASE COUNT 984 a 590 c 689 g 954 t
ORIGIN 673 bp upstrem of PvuII site.
1 tcttggtagg ttgttgatac tatcatttat tacccatttg ttaaggtata cgatgaacaa
61 agttcttgaa gaaagaaagt tctggtaaaa caaacgatgc tcttaaagaa aaagtagctg
121 caaacttcaa tactgctaaa gcagagatgc cgttcttgga aaagcagacg ttgctaaaga
181 agatgtagca gcaaacaata atatcacaaa agaaactaat gtccttgttc tttgtgcagg
241 tggaggtaca agtggcttgc ttgccaatgc cttgaacaaa gcagcagcag aatacaatgt
301 tccagtcaag gcggcagcgg gtggttacgg tgctcaccgt gaaatgttgc cagagtttga
361 cttggtaatc ttggctccac aggtcgcttc aaacttcgat gatatgaagg ctgaaacaga
421 taaattgggc attaaacttg taaaaactga aggagctcaa tatatcaaat tgacacgtga
481 cggacaaggt gctcttgcat ttgttcaaca acagtttgat taataaattt gtaaaagtta
541 tttgagacga gatcactcat tccatttcct tggttttcag aacacagaat ctcgtctcaa
601 atgctttttt tgaaaggact tacacttatg actaaaacac ttcctaaaga ttttattttt
661 ggtggtgcta cagctgctta tcaagcagaa ggtgcaactc acacagatgg taagggccca
721 gtagcatggg ataagtatct tgaagacaat tattggtata cagcagaacc tgcaagtgat
781 ttttaccata aatacccagt tgatttagaa ttagctgaag aatatggtgt taatggtatt
841 cgtatttcta ttgcctggtc tcgtattttc ccaacgggtt acggtgaagt aaatgaaaaa
901 ggtgttgaat tctaccataa actatttgct gaatgccaca agcgtcatgt tgaaccattt
961 gtgactttgc atcattttga cacaccagaa gctcttcatt caaatggaga tttcttaaac
1021 cgcgaaaata tagaacactt tatagattat gccgctttct gttttgaaga gtttccagaa
1081 gtaaactact ggacaacttt caatgaaatt ggcccaattg gtgatggtca atacttggtt
1141 ggtaaattcc ctccaggcat taaatatgat cttgccaaag tcttccaatc acaccacaac
1201 atgatggtct ctcatgcacg tgctgtaaaa ttgtataagg ataaaggtta taaaggtgaa
1261 attggtgttg ttcatgcttt accaactaaa tacccttatg atccggaaaa tccagcagat
1321 gttcgtgctg ctgaacttga agacatcatc cataacaaat ttatcttgga tgcaacctat
1381 cttgggcact attcagataa aacgatggaa ggtgtcaacc atatcctagc tgagaatggt
1441 ggagaacttg atcttcgtga tgaagacttc caagctcttg acgcagctaa agatttgaat
1501 gatttccttg gtatcaacta ttacatgagt gattggatgc aagcttttga tggtgagact
1561 gaaatcattc acaatggtaa gggtgaaaaa ggaagctcta agtaccaaat taagggtgtt
1621 ggtcgtcgag tagctcctga ctatgttccg cgcacagact gggattggat tatttatcct
1681 gaaggcttgt atgaccaaat catgcgagtg aaaaatgatt atccgaatta caagaagatt
1741 tacatcactg aaaacggtct cggatacaaa gatgagtttg tagataatac tgtttatgat
1801 gatggtcgta ttgattacgt gaagcaacac ttggaagttt tatcagacgc gattgcggat
1861 ggtgcaaatg ttaaaggtta cttcatttgg tcacttatgg acgttttctc atggtcaaat
1921 ggttatgaaa aacgttatgg attgttctat gtagactttg atacgcaaga acgctatcct
1981 aagaaatcag cacattggta taagaaatta gcagaaactc aagtgataga gtaatatcac
2041 ctgagaatgt aataatttgt tatctgattt ttagatcagg ctttgaaaag gtatatatta
2101 ttaatttaga agattgtcct ataattcctg agcttgaagc tacctcacta caaaatgtag
2161 taactggtat gtttcacctg gaaaagaaag gatttgccat tctgatttag cattatccga
2221 atttgaatgg gaagttttac aattactcga tgtagcaata tgtgagactg ttcacttcga
2281 ggtagattat ttgcctttcc aataacaaaa agaaaggaat tgactgtgac tatcgaatta
2341 aaaaacgagt atcttacggt tcagtttaaa acacttggtg gtcaactgac ttcgattaaa
2401 gacaaagatg ggttagaata tctctggcaa gctgatccag agtattggaa tggacaggca
2461 ccaattctat ttcctatctg tggtagttta cgtaatgatt gggctattta tagacctcaa
2521 gaaagacctt tttttacagg tctaattcgt agacatggtt ttgttcgaaa agaagaattt
2581 actcttgaag aagttaatga aaatagcgtg actttcagta ttaaaccaaa tgctgagatg
2641 cttgataatt acctttatca gtttgaacta agagttgttt ataccttaaa tgggaaatcc
2701 ttacttcatt ggagcccatc cagcattcaa ttgtccttta gtagaaggcg aaaagtatga
2761 ggactattct cttgaattta gtgaggttga gtcttgttcg ataccaaaga gtttccctga
2821 gacagggtta ttagatttac aagatcggac gccatttttg gaaaaccaaa agtctcttga
2881 tttggattac tcactttttt ctcatgacgc cattacactt gaccgactga aatctcgctg
2941 cgtgacactg tctcgacgct ctcccttatg cgactcctgc attagaagca gcccagtagt
3001 aggttaggcc gttgagctcc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
3061 acagtcccgc ccacggggcc tgccaccata cccacgccga catgagcccg aagtgcagcc
3121 cgatcttccc catcggtgat gtcggcgata tagcgccagc aaccgcacct gtggccgcgg
3181 tgagcctcga acgatgcgtc cggcgtagag gatcccc
//