GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS YSCGDHM 1840 bp ds-DNA PLN 23-SEP-1990
DEFINITION Yeast (S. cerevisiae) GDH1 gene encoding NADPH-dependent glutamate
dehydrogenase.
ACCESSION M11297
KEYWORDS dehydrogenase; gdhA gene; glutamate dehydrogenase.
SOURCE S. cerevisiae DNA, clone pGDH1.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 1840)
AUTHORS Moye,W.S., Amuro,N., Rao,J.K.M. and Zalkin,H.
TITLE Nucleotide sequence of yeast GDH1 Encoding nicotinamide adenine
dinucleotide phosphate-dependent glutamate dehydrogenase
JOURNAL J. Biol. Chem. 260, 8502-8508 (1985)
STANDARD simple staff_review
COMMENT [1] notes mRNA start sites by S1 mapping to be at positions 160,
164 and 172.
FEATURES from to/span description
pept 228 1592 glutamate dehydrogenase
mRNA 172 > 1592 GDH mRNA [1]
site 1 60 putative VECTOR sequence pBR322
BASE COUNT 566 a 341 c 395 g 538 t
ORIGIN 250 bp upstream of HindIII site.
1 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
61 gcatcaagcc aggcgttccg ggagtatttc ctaaaagttg ccaaagatta tcaaaatatt
121 accgtgttta taaaataaac tgacttttct tatagcattg aaagttgctt gatacgtact
181 atcgcattat tctaatataa cagttaggag accaaaaaga aaaagaaatg tcagagccag
241 aatttcaaca agcttacgaa gaagttgtct cctctttgga agactctact cttttcgaac
301 aacacccaga atacagaaag gttttgccaa ttgtttctgt tccagaaaga atcatacaat
361 tcagagtcac ctgggaaaat gacaagggtg aacaagaagt tgctcaaggt tacagagtcc
421 aatataactc cgccaagggt ccatacaagg gtggtctacg tttccatcct tccgggaact
481 tgtctatctt gaaattcttg ggtttcgaac aaatcttcaa gaactccttg accggcctag
541 acatgggtgg tggtaaaggt ggtctatgtg tggacttgaa gggaagatct aataacgaaa
601 tcagaagaat ctgttatgct ttcatgagag aattgagcag acacattggt caagacactg
661 acgtgccagc tggtgatatc ggtgttggtg gtcgtgaaat tggttacctg ttcggtgctt
721 acagatcata caagaactct tgggaaggtg tcttaaccgg taagggtttg aactggggtg
781 gttctttgat cagaccagaa gccactggtt acggtttact ttactatact caagctatga
841 tcgactatgc cacaaacggt aaggaatctt tcgaaggtaa gcgcgtcacc atctctggta
901 gtggtaacgt tgctcaatac gctgccttga aggttattga gctaggtggt actgtcgttt
961 ccctatctga ctccaagggt tgtatcatcc ttgaaactgg tatcacctcc gaacaagtcg
1021 ctgttatttc cagtgctaag gtcaacttca agtccttgga acaaatcgtc aacgaatact
1081 ctactttctc cgaaaacaaa gtgcaataca ttgctggtgc tcgtccatgg acccacgtcc
1141 aaaaggtcga cattgctttg ccatgtgcca cccaaaatga agtcagcggt gaagaagcca
1201 aggccttggt tgctcaaggt gtcaagttta ttgccgaagg ttccaacatg ggttccactc
1261 cagaagctat tgccgtcttt gaaactgctc gttccaccgc cactggacca agcgaagctg
1321 tttggtacgg tccaccaaag gctgctaact tgggtggtgt tgctgtttct ggtttagaaa
1381 tggcacaaaa ctctcaaaga atcacatgga ctagcgaaag agttgaccaa gagttgaaga
1441 gaattatgat caactgtttc aatgaatgta tcgactatgc caagaagtac actaaggacg
1501 gtaaggtctt gccatctttg gtcaaaggtg ctaatatcgc aagtttcatc aaggtctctg
1561 atgctatgtt tgaccaaggt gatgtatttt aaatagtcta aaagaaagaa aagaggaaag
1621 ttcataaaaa gttctttctt ttttttaaat aacttagata gataatgaga taaaagaagt
1681 aaaaaaacgg aatcgtaacg caattaatta attaagaaaa ttgattatcg ggagaaaaaa
1741 attttatttt ttattttatt ttctttaaag tacatatatt tgaaatgatg acatgtggaa
1801 atataacaga gacagaatat ttaattttat gtatgtaagc
//