GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS YSCSEC7 6753 bp ds-DNA PLN 23-SEP-1990
DEFINITION Yeast (S.cerevisaie) Sec7 gene encoding a protein required for
membrane traffic from the yeast Golgi apparatus, complete cds.
ACCESSION J03918
KEYWORDS .
SOURCE Yeast (S.cerevisiae) cell line X2180 DNA, clone pCF36.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 6753)
AUTHORS Achstetter,T., Franzusoff,A., Field,C. and Schekman,R.
TITLE SEC7 encodes on unusual, high molecular weight protein required for
membrane traffic from the yeast Golgi apparatus
JOURNAL J. Biol. Chem. 263, 11711-11717 (1988)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly provided
by A.Franzusoff, 30-JUN-1988
FEATURES from to/span description
pept 439 6468 Sec7p protein
site 6577 6729 putative VECTOR sequence pBR322
BASE COUNT 2228 a 1248 c 1335 g 1942 t
ORIGIN 390 bp upstream of EcoRI site.
1 ctcttaagat atacacttta gtacttttga agctcttaca gttttttgat gtcggagaag
61 gcttatacat attgttattt tccctctaca ggaaagaagg tgaacgttta gtttttatca
121 aggcgccgag taaattttat aatatcgagg cgagggaaaa acaaagcaac gagaaaggaa
181 gctcaaagtt gggatttaca tcatttcttt aaaaagcatc attggcttct ctccaattgt
241 tcaataaacg cagactaata gaaattgaag tcattttccg gagaaatata aaatattttc
301 tgcaactaag ctatactcga tttcttttaa gtttctccag aaccaataaa aagtaatatc
361 ttaaactgtt tctttctcat tttttccccg aattccatta aagaagataa gaaacaagtc
421 taagtgctac tatatctgat gtctgaacag aattcagtag taaatgctga aaaaggggat
481 ggcgaaatat cttcaaatgt agagactgct tcttcagtca atccgtctgt gaaaccacag
541 aatgctatta aagaagaagc taaagagaca aatggtgaag atcaaaaatg caaagggcca
601 gaaaacgctg gcagtacggc agaaacaaaa gaaacaagta acgacgctac caacggaatg
661 aaaacgcccg aagaaacgga agatacaaat gacaaacgcc atgacgatga aggtgaagat
721 ggagatgaag atgaagatga agatgaagat gaagatgaag acaatgggga cgaggatgat
781 gaggacgtgg atagcagtag ttccgaaacc agttccgaag acggggaaga ttctgaatca
841 gtctcagggg aaagtactga gagtagctct ggagaagatg aagaatctga tgaaagcgat
901 ggaaatacat caaacagctc ctctggtgat gaaagcgggt cagaagaaga agaagaagaa
961 gaagaagaag aagaagaaga ggaaaatgct ggcgaaccca gtatcgctca tcaagatagt
1021 gttcccacca acgattctac tgccccaagg tctacccata cgaggaacat atcactatct
1081 tcaaatggtt cgaacacaaa ctcaaccata attttagtga aaactacgtt ggagacaatt
1141 ttaaatgata aggacattaa aaagaattcg aatgctcaga aggctatcga aaggacatta
1201 caaaaattta aggaatttga tccgcaaacc acgaataacc cacattacgt ggattcaata
1261 ctagtattcg aagcactaag ggcaagctgt cgtaccaaat cctccaaagt tcaaagtttg
1321 gctttagatt gcctgtcaaa attgttttct tttagatcgc tagacgagac cctgttagtg
1381 aatccacccg attctttagc ctctaatgat caacgacaag atgctgccga tggaataacg
1441 ccgcctccaa aacaaaaaat tatagacgct gcaattgata ctatttcaga ctgttttcaa
1501 ggtgaaggca ctgatgaccg tgtggaacta caaatcgtta gagctttatc tagttgcatt
1561 ttagaagagg attcaagttc tttatgccac ggtgcttcct tgctaaaggc tatcagaaca
1621 atctacaacg ttttgctctt ttgcttgaac ccatccaatc aaggtattgc acaggcgacc
1681 ttgacacaaa ttattagttc ggtgtatgat aaaatcgatc tcaaacaaag tacctcttca
1741 gcagtatcct tatcgacaaa aaatcatcaa caacaatccg ccatagaact ttccgaggct
1801 tctgaaaatg cagaaacgcc ggctccttta actctggaaa atatggataa gttgaatgac
1861 gatgaggaaa gactaatgga cgctcaacag cctgattcta tcgccataac taaccaagat
1921 ctagctgtta aagatgcgtt cttagtgttt agagtcatgg cgaaaatatg tgctaaacct
1981 ttggaaacag aactcgacat gaggtcacat gccgtcaggt caaagctttt atctcttcac
2041 atcatttact ctattatcaa agatcatatt gacgtattcc tttcccacaa catttttcta
2101 ccaggaaagg agcgtgtgtg ctttatcgat tcaattagac aatatttacg tcttgtttta
2161 tcaaggaatg ctgcctcgcc tctagctcca gttttcgagg ttactttaga aattatgtgg
2221 ctattgattg ctaatttgag agcagatttc gtgaaggaaa ttccagtttt tttaacagaa
2281 atctacttcc ccatttcaga attaaccact tccacctccc aacaaaagag atatttttta
2341 agtgttattc aacgaatttg taacgaccca agaactttag ttgaatttta cttgaattat
2401 gattgtaacc ctggaatgcc aaatgtaatg gaaataactg ttgattattt gacaagattg
2461 gctttaacta gggtggaaat tactcagact caaagatcat actacgatga acaaatatca
2521 aaatccctgt ccacctacaa tttctcacaa ttgccccttt tgacttcttc caacttgtct
2581 tctagtcctg atgttggtca agtcaattta ctttttccac ttgattttgc tctcaaaatg
2641 gtgtcactaa attgcattgt gtcagttttg cgttccttaa gctcatgggc tcacaaagct
2701 ttaaatccaa acacacacac tgctaataag gtattactaa acacaacatc ttcagctcgt
2761 caagaatcta gatcatcttt gagtaacgac gtaaggtctt ctattatgac aagtaatgat
2821 gacttcaaac caacatatga agacgaagaa agcagatcat tgagcagtca aaacattgac
2881 gcagacgacc ccacacaatt tgaaaatttg aaactaagga aaactgcttt atcggaatgt
2941 attgctattt tcaacaataa acccaagaaa gctattccag tacttattaa aaaaggattt
3001 ttgaaagatg attctccaat ttccattgcc aagtggttat tagaaacaga aggcctcgac
3061 atggccgctg ttggggatta tctaggcgaa ggagacgaca agaacatcgc tataatgcac
3121 gcatttgttg atgagtttga cttcactggt atgtccattg ttgacgcatt aaggtcattt
3181 ttacaaagtt tcagattgcc tggagaaggt caaaaaattg acagattcat gctgaaattt
3241 gcggaaagat ttgtggacca aaaccccgga gtcttttcaa aggcggatac tgcatatgtg
3301 ctttcgtatt ctttgatcat gttgaatact gatttacatt cgtcacaaat caaaaataaa
3361 atgtctttac aagagttttt agaaaacaac gaaggtattg acaacggaag agatttacca
3421 agagacttct tggaaggttt gttcaacgaa attgctaaca atgaaatcaa gttaatttct
3481 gaacagcatc aggcaatgct ttcaggtgat accaatcttg tccaacaacc agcaatctgc
3541 tttcaactct ttaattctcg tgatttgaca agagaggcat ataatcaagt ctcaaaagaa
3601 atttcatcta aaacggaatt agtctttaag aatttaaaca aaaataaagg aggcccagac
3661 gtctattatg ctgcttccca cgttgagcat gttaaatcaa ttttcgagac actatggatg
3721 tcctttttag cagctctaac ccccccattt aaggattatg atgacattga cacaaccaat
3781 aagtgtttag aaggcttgaa aatatcaatt aaaattgctt ctacttttag aatcaatgat
3841 gctagaacct cctttgtagg tgcgttagtc caattttgta accttcaaaa ccttgaagaa
3901 atcaaagtta aaaatgtcaa tgcaatggta attcttcttg aagtcgcgtt atcagaagga
3961 aattacttgg agggatcgtg gaaggacatt ttgctggtcg tgtctcaaat ggaaagacta
4021 caattgatat ccaaaggtat cgacagagat acggttccag atgttgcaca agctcgtgtt
4081 gcaaacccta gagtttctta cgaatcatca agatccaata atacatcttt ctttgatgtt
4141 tggggcaaga aggcaactcc cacagaattg gctcaagaaa aacaccataa tcaaacctta
4201 tcacccgaaa tctctaaatt catttcctcc agtgaattag tcgttttgat ggacaatata
4261 tttaccaaaa gttccgagtt atcaggtaat gctatcgttg attttatcaa agctttaacc
4321 gctgtatctt tagaagaaat tgaatcatct gaaaatgctt ccacaccaag aatgttttcc
4381 ttgcaaaaaa tggtcgatgt atgttactac aatatggatc gtatcaaact agaatggacg
4441 ccgctttggg ctgttatggg aaaagctttc aacaagattg ctacaaactc taacttagca
4501 gtagtatttt tcgctatcga ttccctgcgt caattgtcta tgagattttt agatattgag
4561 gaattatcag gttttgaatt tcaacatgat tttttaaaac cttttgaata cacggttcaa
4621 aatagtggca acactgaagt tcaagaaatg attattgagt gttttaggaa cttcatattg
4681 acaaaatcgg aaagtatcaa atctggctgg aagcctattc ttgaatcctt acaatatacg
4741 gctcgctcaa gcactgaatc cattgtatta aagacgcaat tgctggttag caatgatatt
4801 gtgacaaatc acttcgaaaa cgtattctct caagaagatg ccttttctga gttagttggt
4861 gtcttcagag aaatcaccaa aaataagaga ttccaaaagc tatctctaca tgctttggag
4921 tctctaagaa agatgactca gaacgtcgca gacatctgtt tttacaatga aaataagact
4981 gaagaagaaa gaaaacataa cgatgcttta cttcgtggga aagacatatt ccaggatgtg
5041 tggttcccta tgttattctg cttcaatgat acgatcatga cagctgaaga cttagaagtt
5101 agatcacgtg cattaaacta tatgttcgat gccctagtgg catatggtgg taaattcaat
5161 gatgatttct gggaaaagat ttgtaagaag ttactatttc ctattttcgg tgtattatcc
5221 aaacattggg aagtcaacca attcaatagt catgatgatt taagtgtttg gctttcgacc
5281 acgttaattc aagccttaag gaacttgatt gcccttttta cgcattactt tgaatcgttg
5341 aacagaatgc tcgatggatt tttgggtctg ctggtatcct gtatttgtca agaaaatgac
5401 actattgcta gaattgggag atcctgcctg caacaattga tattgcaaaa cgtatctaaa
5461 ttcaatgagt accattggaa tcaaataggt gacgtattcg ataaattgtt tgatttaacc
5521 actgctaatg aattgttcga ttatgatccg ttacagcaag gaagaaaatc atctgtatct
5581 catcaccaaa caactaatga taccagccaa cactcagatg atgatagcaa cgacagaagg
5641 gagaacgatt ctaatatcag tgagacggtc gaaagagcgc atcaagaaga atccagcgaa
5701 gacgttggtg gtgacatggt tgaaacactc aatgggcaaa ctaagcttaa taatggcaat
5761 tccgttccga cggtaaagga cgaattgaat ccaaagcccg ctagtctaag tatccccaag
5821 aaaactaagc acatgaaacg aaacgaaagt aacgaagata tacgtaggag aataaacatc
5881 aagaattcta ttgttgtcaa atgtgtttta cagcttttga tgatagagct gctcaacgaa
5941 ttattcgaga acgaagattt tgcccactgt atcccttaca aggaagccat tagaattaca
6001 agattgttgg agaaatcgta cgaattttct cgtgacttta atgaagatta tgggttaaga
6061 acaagactag tagaggctcg tgtagttgat aaaataccaa acctgttgaa acaagaaacg
6121 agtgctgctg cagttctcct tgacattatg tttcagttgt atctgaacga tgatgagaag
6181 aaggctgatt taataactcg tctgatcacc atttgcatcc aggtcgtaga gggttatgtt
6241 tctttggatg atagaaccat ggaacgtagt atcaatgcct ggcgttcagt gatagttgaa
6301 atattacaag gttactacga atttgatgat gaagacttta gactatactg tcctgctatg
6361 tatgctctgg tgattcaaat attagataag agcgttccaa cggagttgag acatgctata
6421 aaacaatttc taagcagagt tggtgaatta tacctttcta ctgattaagg ctttgttgaa
6481 tggtccagca gattaaaata tgcttagttg tagaacacag aaaaaatgag aaggttgata
6541 tacttttact catattaatc ctgatttagt ggcggtgatc ctctacgccg gacgcatcgt
6601 ggccggcatc acgggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
6661 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
6721 tgcaggcccg tgccgggatg ttgcactctc ttc
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