GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS YSKLAC12 7127 bp ds-DNA PLN 23-SEP-1990
DEFINITION Kluyveromyces lactis LAC12 gene for lactose permease.
ACCESSION X06997
KEYWORDS lactose permease; unidentified reading frame.
SOURCE yeast (Kluyveromyces lactis).
ORGANISM Kluyveromyces lactis
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 7127)
AUTHORS Chang,Y.D.
JOURNAL Unpublished (1988) U of Kentucky Med Center, Lexington, KY
STANDARD simple automatic
REFERENCE 2 (bases 1 to 7127)
AUTHORS Chang,Y.D. and Dickson,R.C.
TITLE Primary structure of the lactose permease gene from the yeast
Kluyveromyces lactis
JOURNAL J. Biol. Chem. 263, 16696-16703 (1988)
STANDARD simple automatic
COMMENT *source: strain=Y1140 (wild type); library=Sau3A; *source:
clone=pCKR1B-LAC4-1; the sequence submitted starts from the 5'end
of LAC4 gene but goes to the opposite direction; therefore, base
number 1 is -1199 of LAC4 gene; for LAC4 gene seq. see Mol. Cell.
Biol. (1987)7,4369-4376
EMBL features not translated to GenBank features:
key from to description
SITE 991 1007 put. upstream activating sequence 2
SITE 1271 1287
TRANSCR ? 1485 transcript
TRANSCR ? 1489 transcript
TRANSCR ? 1494 transcript
TRANSCR ? 1496 transcript
FEATURES from to/span description
pept 1616 3379 lactose permease (AA 1-587)
ORF 3949 5217 ORF II
site 6786 7127 putative VECTOR sequence pBR322
BASE COUNT 1970 a 1436 c 1490 g 2231 t
ORIGIN
1 gcgacttgga aggccaaagg actatcgcat gattattcgc cgggaacccg cgagttccct
61 gctcttttct ttcaaaccag gcagcaaacc aggtgaacac actctgatgt agtgcagtcc
121 ctaagtcctt tgaagattcg gggagctagc tacccacgcg aatgtaacaa aagaacattt
181 acttttgtgg ggggtggaaa agtcgattag gatcttgcag cacagaaact gcgcaggggt
241 ttttttcatc ttggagaagc aactggctaa attcgacaca aacaaaaact gaaaaatgga
301 aaataaaaaa tgaaaaagca agctgaattc gaagaaggga gaattccgcc ttctgcaacc
361 acactaatgg ttggtagtca atagatacgc attagaaggt tactatttta tgagtcgatc
421 cccgcggaaa tttaggaatt ttaaacttgg gcttgatata cgtcggtata gtgtatctgg
481 tatttttgcc aatggttcct gtgcccgtgc ccgtgcccgt gtgctcatgc gatgcattgt
541 gactactgct tggcaaggca aatcagcggt ctgcggccgt actctaaagc cggacagaca
601 tggtggggtg ccttttcggt ccataagtgt aactagctag ctacaagtat gttctgtgca
661 aagatggtaa aggttgcata cagtcaagtt gctgtccttg gacctggtct gacctgacct
721 agcttaacat tgcatagcgc atacccactt ggatggatgc aggagtgtta caaatcggcg
781 aacagttctc cttgtttgtt tttggtcata gtcacgtccg gctacacaca agatatggca
841 cagtatgtgt agatgcgagc gaagatacgg ctggaaaaca ggtgggtttt gaaggataga
901 cagaaggcaa gagggtgggc aaaaagtcgg gcactcaggc aagtattggc atgatcaaac
961 cagattaagt tctaggccaa gaacatattt cggcccactg actcccgcca aaaaaaaaga
1021 aaaaaaccta atttgacagt ctcggaattt tatctatctg ttcctttcct ttggttacta
1081 gtatcgtcat ttaatttttt ttggaattgg caagttacct gcgtggctct ataccgaatg
1141 aaccccgacc ctggccgttc ggtgcaccga gcgcgtttac gtgtgtacac gcttttgtaa
1201 gaaagaaact cgggtctgcg tgggcctgca acgtgactta gcaatacccg cgcgtcagat
1261 ttaacatatt cgggattctt ccctccgtac tagaaagttg acaataagat gtccatactg
1321 ttgttgatac cttgctactc tagctcatta tgacaaggac aatcggttaa ttggaggtct
1381 aaatggtgta tcaaggtata taaaggcagt aactttcaat gttgagttgg gctgtagttc
1441 aaggtggttg tagcttctgg aacatatata tgctattcaa tatagctgga aagtgtaagc
1501 taaacagcaa acaaaagtct ctgtgtgttg tcttataatt atatccttca gtcagtgtta
1561 atgattaaag acagaattac gaaaaaataa aacaagacga taatcagttt tcaaaatggc
1621 agatcattcg agcagctcat cttcgctgca gaagaagcca attaatacta tcgagcataa
1681 agacactttg ggcaatgatc gggatcacaa ggaagccttg aacagtgata atgataatac
1741 ttctggattg aaaatcaatg gtgtccccat cgaggacgct agagaggaag tgctcttacc
1801 aggttacttg tcgaagcaat attacaaatt gtacggttta tgttttataa catatctgtg
1861 tgctactatg caaggttatg atggggcttt aatgggttct atctataccg aagatgcata
1921 tttgaaatac taccatttgg atattaactc atcctctggt actggtctag tgttctctat
1981 tttcaacgtt ggtcaaattt gcggtgcatt ctttgttcct cttatggatt ggaaaggtag
2041 aaaacctgct attttaattg ggtgtctggg tgttgttatt ggtgctatta tttcgtcttt
2101 aacaacaaca aagagtgcat taattggtgg tagatggttc gtggcctttt tcgctacaat
2161 cgctaatgca gcagctccaa catactgtgc agaagtggct ccagctcact taagaggtaa
2221 ggttgcaggt ctttataaca ccctttggtc tgtcggttcc attgttgctg cctttagcac
2281 ttacggtacc aacaaaaact tccctaactc ctccaaggct tttaagattc cattatactt
2341 acaaatgatg ttcccaggtc ttgtgtgtat atttggttgg ttaatcccag aatctccaag
2401 atggttggtt ggtgttggcc gtgaggaaga agctcgtgaa ttcattatca aataccactt
2461 aaatggcgat agaactcatc cattattgga tatggagatg gcagaaataa tagaatcttt
2521 ccatggtaca gatttatcaa accctctaga aatgttagat gtaaggagct tattcagaac
2581 gagatcggat aggtacagag caatgttggt tatacttatg gcttggttcg gtcaattttc
2641 cggtaacaat gtgtgttcgt actatttgcc taccatgttg agaaatgttg gtatgaagag
2701 tgtctcattg aatgtgttaa tgaatggtgt ttattccatc gtcacttgga tttcttcaat
2761 ttgcggtgca ttctttattg ataagattgg tagaagggaa ggtttccttg gttctatctc
2821 aggtgctgca ttagcattga caggtctatc tatctgtact gctcgttatg agaagactaa
2881 gaagaagagt gcttccaatg gtgcattggt gttcatttat ctctttggtg gtatcttttc
2941 ttttgctttc actccaatgc aatccatgta ctcaacagaa gtgtctacaa acttgacgag
3001 atctaaggcc caactcctca actttgtggt ttctggtgtt gcccaatttg ttaatcaatt
3061 tgctactcca aaggcaatga agaatatcaa atattggttc tatgtgttct acgttttctt
3121 cgatattttc gaatttattg ttatctactt cttcttcgtt gaaactaagg gtagaagctt
3181 agaagaatta gaagttgtct ttgaagctcc aaacccaaga aaggcatccg ttgatcaagc
3241 attcttggct caagtcaggg caactttggt ccaacgaaat gacgttagag ttgcaaatgc
3301 tcaaaatttg aaagagcaag agcctctaaa gagcgatgct gatcatgtcg aaaagctttc
3361 agaggcagaa tctgtttaaa gccatctttt caatatattt tgttaggtgc aagaagtttc
3421 cgtacttcat aatttgtttt ttattctgct tgatcttctc ctaattgcag caaaaagtct
3481 tgtggaattc atcaattaaa aagccacagg ctttagactc ttaatggatt attctaacag
3541 ttactgttag acgttagcac atgggccggt atgtcattga agtacgttta aattgcctga
3601 attgggaaaa gtattgacct ttgagccggt tgcataccgc cttgcggtat gcagtttggc
3661 tcggttttcc cagcacgcat gtgggcatca tttccacacg tgtgaaccct cggcagttaa
3721 atgtgtgcat ttaggatcgg ctaatagttg tttttagctt cagcttcagc tcatcggatt
3781 ttgtgaaaca tataaatctg gcgtcaattg agtctctcga atggttgaaa ggtcaattca
3841 ggttggaatt ttgtattatg ttagctatat gggattgatc aaaaaaacca gccaaggatt
3901 cagagaatta cagcgcaagc tcaggtagca ctccagtttt aaacataaaa aatgaaattc
3961 tccgatttta gtgttctcgg attaggtgct cttgccttaa atgccgttac cgtcagtgct
4021 aacactgctg acaccgcttt gttgagaact tactctacta tctctccaag tttgagtgaa
4081 attgaatccg ccgcttctgc tactgaggtt gctgaggtgg tttcagatgt cgaaggtgcc
4141 gctttcaaga gattcttcat tatcttcttg gaaaacaccg attacgacaa ggctgctggt
4201 gatgaatccc tttcatggct ggctgaacaa ggtattacct tgaccaacta ctgggctttg
4261 actcaccctt cagaaccaaa ctatttggct tctgtcggtg gtgattactt cgccttggat
4321 gatgacagat ttatttctat gccatccaat gtgtctaaca tcgttgattt gttggacact
4381 aagggtatct cttgggctga ataccaagaa cactctccat atgctggttt ccaaggtatg
4441 aacttctcta accaagagac atacgccagt gattatgtca gaaaacataa cccattgatt
4501 ttgttcgata acgttgtcaa caacgatact cgtttggcta acattaagaa ctttgaagat
4561 ttcaacaatg acgtagaaaa cgaaaagtta cctcaatacg ctttcattac tccaaatatg
4621 actaacgatg gtcatgacac cactatccaa tttgctggta aatggtcaaa ggacttcttg
4681 gctccattat tagaaaacga ttacttcatg gaggacactt tggtcttgtt gaccttcgat
4741 gaaaatgaaa cctatggtat caagaacaag gtcttctcca tcttgttggg tggcgttatc
4801 ccagatgaat tgaagggtac caaggatgat actttctatg accattactc tcaattggct
4861 agtgttgaag ccaactggga tttgcctcat ttaggtagac acgatggtga cgccaatgtt
4921 ttggaaattg ttgctaacgc taccaacatc accaacgttg aagttgacac cacttacatg
4981 atcaacgaaa cctatattgg ttacttgaac gattacaaca ttgaattgcc agctccaaat
5041 gttactgcta ttaacagaaa cggtcaacca atcttggact ccatcaagga aacttgggaa
5101 gatgaatatt ctaagcaagt ctctgaatcc tactatactt ccaccactac taccgtttct
5161 gcagatgtta ctgatgctga aaccttctcc aatttctacc gctaccgaca gtgctgatga
5221 aactagtgct accgctacat cttcttccaa cagcacttct gctgaaactt taagctcttc
5281 cgccggtgct ccatcaatga gcacttctcc ggtaacgtct tgttgggtgt cattgctgcc
5341 tttttcttat gaaaagatat cgactcaaag gagtagcagc acactcctat agagaaacta
5401 gagtgaaaac ggcagttcac tcttagttaa tattatttat tccgtacaaa tagaacagtt
5461 gaaaagtgac gcttttataa aataagaact tgattttgat ttgacctttt aattattaac
5521 cttatcattg aataataagt attatttcct tttacttgat atcttcttag tcttgacaac
5581 tgaaatattg tacgtctgtc ttgaaattta gctagtggtt caagaaccgt cataggaaaa
5641 aatacctatt ttttgtggta tgctgttagt aatgaatata tgttttgatt gtatatctaa
5701 tcgagaaaaa gaaatgcaga atgacagaga ctcctgtctg gtttcaatgt catgaaaagt
5761 tgtgcataag caaacttaac aattagataa gatggaaaag ttcaagtact cccctcccca
5821 ttgttgattc tgaattgtaa taccttgtcc taatcggaat tttcatgaca agagggtcaa
5881 gcccaacttt cttttaatgc cctagtcact aatatctcat tttcattttc cgcagttaga
5941 ttggttagat gtggcagaat ttttggaacg aacctcccgt gggttgtcta atattaaata
6001 cagacacttt ataaccagta ttcttttttt ttccttatta ttctcattta tttctctcat
6061 tacttttgtc caggaatatc ttaactctgg catcaatcac tgaagcaaag ttccacgtcg
6121 gggttcggca gtaaacccat actgtctttc gtgccctcct tataaacttt tcacctaagt
6181 aaacattagt tcttagtatt ccaaatttcg tacaccagct atcattataa cgatcagtat
6241 actgcaaagc ttcaaaagtt gagagttgac gtgccttccc taacaaaacc catcccatat
6301 cctttttgtc caacatctca aactttattt cagttttgtc tagtttctgt gttatgcaga
6361 taagctgtgc atataaatgt tggaaataag ctaagctgtt acgttttgtt ctcagttgtt
6421 agcaagagca gtaaatcaaa aatgattgtt cttttggaac cacccaagta aaaaacatat
6481 ccacatctgt aagtgtccgc gataaaaccg aacccgaaag tgagattcct cccattatta
6541 cgcacgaagg agttattctc atcttgtcag tagccttatc cgtatccgct tttttattat
6601 taccgtgaca ttcactaagc tattttgcta gcccactcct aacacatagc gttttgagcg
6661 tcttgggtac ttactatgaa aatgacagtt aactactttt gactagtcac aggttttaga
6721 tcaaagaatc ttgggaaaaa gatcgtattt tatagttaat tttattgttt catatctatt
6781 attttgaggt gctttacgga ctcctcgacc gatgcccttg agagccttca acccagtcag
6841 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
6901 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt
6961 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
7021 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat
7081 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcg
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