GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS LACPPWV05A 1200 bp ds-DNA BCT 23-SEP-1990
DEFINITION L.lactis (strain Wg2) proteinase plasmid pWV05 DNA.
ACCESSION M37396
KEYWORDS proteinase.
SOURCE L.lactis (strain Wg2) DNA, clone pWV05.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1200)
AUTHORS Haandrikman,A.J., Van Leeuwen,C., Kok,J., Vos,P., De Vos,W.M. and
Venema,G.
TITLE Insertion elements on lactococcal proteinase plasmids
JOURNAL Appl. Environ. Microbiol. 56, 1890-1896 (1990)
STANDARD simple staff_entry
FEATURES from to/span description
pept < 1 21 prtM gene
pept 171 341 ORF-W1 protein
pept 1018 338 (c) ORF-W2 protein
binding 158 163 ORF-W1 ribosomal binding site
binding 1031 1026 (c) ORF-W2 ribosomal binding site
signal 105 110 ORF-W1 -35 region
signal 127 132 ORF-W1 -10 region
signal 1063 1058 (c) ORF-W2 -10 region
signal 1085 1080 (c) ORF-W2 -35 region
BASE COUNT 368 a 221 c 207 g 404 t
ORIGIN
1 aagcttgcga caacgaatta aggcttaacc gagtaagatg aaataacagc cagttctttt
61 agaaattggc tcttcgtcaa gtagagttga tagatctggt tctgttgcaa agttttccaa
121 aaaatctatt ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt
181 ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt
241 acaatctaag ctatcgtgaa gttcaggaat tgttatatga tcgggttctg ttgcaaagtc
301 tgaaactcaa aaacactatc tctgaccaac cgtcaatcta agcaggtatt cccattaata
361 ttttgatttc agtagaaact gaaaagccga agagtgttcc atttcttcgg ttctttttat
421 atattcctcg aatggtctcc atccccttaa tcgtggatga ggcagtccgg agacttcgat
481 aaaatttatt ccgtctcttt actggacgat ggtcttgttc aatcaaatta ttcaggtatt
541 taatggtacg atgttctgtc ccttgataaa agccgtattc ttttagtttc ttaaaggcac
601 ttgtaataga gggggcttta tctgtgacta caaccttcgg ttcatcaaac tgcttcacta
661 accgcttaag aaaagcatag gctgcttgtg tgtcccgttt tttacgtaac caaatatcca
721 aggttaaacc atctgcatcg atggctcgat acaaataatg ccattttcct ttaattttga
781 tgtacgtttc atccattttc catgaataaa aggatttttt atttttcttt ttccaaattt
841 gatagagtag tttgccatat tcttgcaccc aacgataaat cttcgtatga gaaacgttaa
901 tgccacgatc atataagatt tcttgaactt cacgatagct aaggttataa cgaagatagt
961 agcccacggc tacaataatc acatcctgct gaaattgctt tcctttaaaa tgattcatcg
1021 tcattcctcc tgctatcttt ttctattatt ctaccttatt tgatagtaga tttaaaactt
1081 tgcaacagaa ccgggcaagg catagtgatg ttatccttaa atcttagagt cactattgta
1141 taatttagac aaaggacaaa aaattgatat tgaattagaa aaatatgttg ctcctgtacc
//