GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS ASTSSP12S 3341 bp ds-DNA PLN 23-SEP-1990
DEFINITION A.sativa 12S globulin seed storage protein gene, complete cds.
ACCESSION J05485
KEYWORDS 12S globulin; storage protein.
SOURCE A.sativa (genomic library lambda-Charon-34), clone lambda-OG1-E1.
ORGANISM Avena sativa
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 3341)
AUTHORS Shotwell,M.A., Boyer,S.K., Chesnut,R.S. and Larkins,B.A.
TITLE Analysis of seed storage protein genes of oats
JOURNAL J. Biol. Chem. 265, 9652-9658 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by M.A.Shotwell, 17-APR-1990.
FEATURES from to/span description
pept 983 1310 12S globulin seed storage protein, exon 1
1428 1702 12S globulin seed storage protein, exon 2
1829 2344 12S globulin seed storage protein, exon 3
2445 2882 12S globulin seed storage protein, exon 4
pre-msg 945 > 3341 12S globulin seed storage protein mRNA and
introns
IVS 1311 1427 12S globulin seed storage protein intron A
IVS 1703 1828 12S globulin seed storage protein intron B
IVS 2345 2444 12S globulin seed storage protein intron C
signal 885 890 CAT box
signal 917 921 TATA box
signal 2915 2920 polyA addition signal
signal 2965 2970 polyA addition signal
site 3251 3341 putative VECTOR sequence pUC18
BASE COUNT 1044 a 677 c 606 g 1014 t
ORIGIN 1 bp upstream of EcoRI site.
1 gaattctttt tggaaagtca ttttgcctcc tgaactccag tgtttcctat ttattaaaaa
61 aaactaaaaa ctatatttat aagtttgaaa aaatcatgaa acaaaattgt aaaaattgct
121 agtgatatat cccacaaacg tgcaaaatct caatttgaag tgctttgtat ttcgagctac
181 acaaaaatga caaagtgtga ctttttatgt gatttgaaat cactatacta cagatctaca
241 attttgttct ttttgtgaag ctataaatac acattatttt gagttgagat ttttcatgtt
301 tgtgctatga atcataggct acatcctgat ttatttttag aattttttgg aacctaaaat
361 atgttctaga ttatttttta aaaagtggga tcattgatgc ccatacacac gaaatctcca
421 ctcaattctt ttatacatta tctttctata tctactaacg tggattatac atcatagtaa
481 gtttcttact acatgtgctt tcttgttaca atgtggacat gactcttcca cttttgggct
541 ttatgttgta tggatatact catgacatgg aattttgtcc acacacgtag aaccatccat
601 atatattgtt gtgcatagaa caaaacacaa gcaagccatg aaaaaggagt cacaggtgcc
661 acaaactgtt gtaggaagta taactagtat gaggcctttt atttgacgtc ggacaatggc
721 caagagctac atataaaaga tggtgctaga tttgtgagtc agcaccagct gtaggcagaa
781 aacaacacat atcttttgag acaaagttat atctattcac atttaaaacc atgatctgtt
841 gagtcaccat gaatatcttc ttatctatgt taataattac atgtcatcat gtttatcctg
901 gactactttt tatggctata aaatcaaact tacaataagg aaactagcac caatccacct
961 tctacaatct cttcaaacaa tcatggcaac tactaggttt ccatcattgt tgttttactc
1021 ctatattttt ctcttgtgca atgggtcaat ggctcagcta ttcgggcaga gctttacccc
1081 atggcaaagc tctcgacaag gaggtttaag ggggtgcaga tttgataggc tacaagcatt
1141 tgaaccactt cgacaagtgg ggtcacaagc gggtatcatt gagtactttg atgagcagaa
1201 tgagcaattt cgttgtgcag gtgtatccgt catccgtcgt gttattgagc ctcaaggcct
1261 cttgttacct caataccaca acgctcctgg cttggtgtac atccttcaag gttagtgtct
1321 aattgattat aaaaattgcc tttgttatac ttcacttagg atttagatgt gccaaatgtt
1381 acaccgttca tattttttaa caatgaaaca aatgtgtttt cttttaggta ggggattcac
1441 agggttgact ttccctggat gcccggcgac cttccaacaa cagttccaac catttgatca
1501 agcccagttt gctgaaggtc aaagccaaag ccaaaatctt aaggatgaac accaaagagt
1561 tcaccacatc aaacaaggag atgttgttgc tctaccggct ggcatagtac actggtgcta
1621 caacgatggt gatgcaccga ttgtagctgt ctatgtcttc gacgtaaaca acaacgctaa
1681 tcagcttgaa ccaaggcaaa aggtaactat acaacttaat gcacacaaaa tatatacaac
1741 tatttacaag tgatccaact atgatttgaa gtatacatta gtgggatatt aatgaactct
1801 gtttaacttc atcgatataa aattttagga gttcttgttg gctggtaaca acaagagaga
1861 gcaacagttt ggacaaaaca tattcagtgg attcagtgtc caacttctta gtgaggccct
1921 tggtataagt cagcaagtag cacaaaagat tcagagtcaa aatgaccaaa gaggtgagat
1981 aattcgtgtg agtcaaggcc ttcaattctt gaagcctttt gtttcccaac aaggaccagt
2041 agagcatcaa gcctaccaac caattcaaag tcaagaagaa caatcaaccc aataccaggt
2101 agggcaatca ccacaatatc aagaaggaca atcaactcaa taccagccag gacagtcatg
2161 ggaccaaagt ttcaatggtt tggaggagaa tttctgttca ttggaggcaa ggcaaaacat
2221 cgaaaacccg aaacgtgccg acacgtacaa cccacgtgct ggcaggataa cacatctcaa
2281 tagcaagaat tttcccaccc ttaacctggt gcaaatgagt gctacaagag taaatttata
2341 ccaggtattt atgatactac attcaacaca ctatcttatt tttagatatt ctaagcttca
2401 tacaaccgat taataatatg gcatacaaat aattgctatt gcagaatgct attctttcac
2461 catactggaa cattaatgct cacagtgtca tgcacatgat ccaaggacgt gctcgagttc
2521 aagttgtcaa taaccatggt cagaccgtat tcaatgacat tcttcgtcgc ggacaactgc
2581 taatcatacc acaacactat gttgttctca agaaggcaga gcgtgaagga tgccagtata
2641 tttcattcaa gaccaacccc aattccatgg ttagccagat cgcaggaaag acctccatcc
2701 tacgtgcatt gcccgttgat gtcctcgcca atgcataccg catttctaga caggaagccc
2761 aaaacctcaa aaataaccgt ggagaagagt ttgatgcatt cacccctaag tttacacaaa
2821 cgggctccca gagttaccag gacgaggggg agtcatcttc gactgagaag gcatccgagt
2881 gaataagtga gtgtaatgga aactagtata gtgaaataaa ggcatcgcat gtttgcagcc
2941 tagtggtata taaccgctta tctcaataaa aaagtttctc cgtgttatat tgtttgcttg
3001 tttcttgtac tcttcttaat tttatctttt atgatcaacc aacttacctc tcttcttcaa
3061 ttttctgcca ctcttcacat atgcatatca aggagacgta tagtccactt gtactagatt
3121 tattttttgg tactatcttt tgtggtttga gtatttctta ggtccgataa aaacattcaa
3181 tttactgatg ctaaattttt tcagcaaacc tagccaccta aaattgtgaa tcacatacct
3241 tcaaatttct atgccaaagt gtgcgatttt tatgttctat agatccaagc ttgggctgca
3301 ggtcgactct agaggatcga tccccgggcg agctcgaatt c
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