GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS FSBSPRL 940 bp ss-mRNA VRT 23-SEP-1990
DEFINITION Chum salmon prolactin mRNA, complete cds.
ACCESSION D00249
KEYWORDS PRL; prolactin; sPRL; salmon prolactin.
SOURCE Chum salmon pituitary gland, cDNA to mRNA, clone psPRL1.
ORGANISM Oncorhynchus keta
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Osteichthyes;
Actinopterygii; Salmoniformes; Salmonoidei; Salminidae.
REFERENCE 1 (bases 1 to 940)
AUTHORS Kuwana,Y., Kuga,T., Sekine,S., Sato,M., Kawauchi,H. and Itoh,S.
TITLE Cloning and expression of cDNA for salmon prolactin in Escherichia
coli
JOURNAL Agric. Biol. Chem. 52, 1033-1039 (1988)
STANDARD full staff_entry
COMMENT The deduced amino acid sequence of the sPRL mature region matched
that of the sPRL polypeptide isolated from chum salmon pituitaries.
A comparison of the amino acid sequences between sPRL and rat PRL
or human PRL is shown in [1]. corresponds to DDBJ (rel4.0) entry
<FSBSPRL>(D00249) dated 05-SEP-1988
FEATURES from to/span description
pept 41 673 chum salmon prolactin precursor (pre-sPRL)
sigp 44 109 chum salmon prolactin signal peptide
matp 110 670 chum salmon prolactin mature peptide
site 15 134 putative VECTOR sequence pUC18
BASE COUNT 258 a 247 c 200 g 235 t
ORIGIN 165 bp upstream of HindIII site.
1 aaggacaaga agataaggag agaagagaaa agaaagaaag atggctcgcc gatcccaggg
61 taccaaactc cacttagcag ttctgtgtct agttgtgtcc tgtcatgcca ttggccttag
121 tgacctaatg gagagagctt cccagcgatc agacaagctt cactcactca gcacttccct
181 caacaaggac ctggactctc acttcccacc aatgggacga gtgatgatgc cacgtccatc
241 tatgtgtcac acctcctcac tccagatacc caaggacaag gagcaagcgc ttagagtatc
301 ggagaatgag ctgatctccc tggctcgctc cctcctgctg gcctggaatg atcccctgct
361 gctgctctcc tctgaggcgc ccactctgcc acacccctcc aatggtgaca tcagcagtaa
421 gatcagggaa ctgcaggact actccaagag cctaggagac ggactggaca tactggtcaa
481 caagatgggc ccctcctccc agtacatttc ttcaatcccc ttcaagggtg gagacctcgg
541 caatgacaag acctcccgcc tcatcaactt ccacttcctt atgtcctgct tccgcaggga
601 ctcccacaaa atcgacagtt tcctcaaggt ccttcgatgc cgggctacca aaatgcgacc
661 agaaacatgt taggagaaaa tgtcagacat tttggttcgg tattgttcta ttttcaaact
721 gatacagagc caaaatgggg tagctgtttg aaggagaatt caggggattg gttattaagt
781 tagattttgt gacatcatga ctaatgctct ctgtcatcaa cattacctgt agactattca
841 tagactataa attaaattca acacctgtca tctgaaccac attttaccag tgtaatatac
901 aactcaagag gttgtattat tgtttatgtt ctgtaatgta
//