GenBank-Updates@genbank.bio.net (09/23/90)
LOCUS SMAPHOAA 2672 bp ds-DNA BCT 23-SEP-1990
DEFINITION S.marcescens alkaline phosphatase (phoA) gene, complete cds.
ACCESSION M33965
KEYWORDS alkaline phosphatase.
SOURCE S.marcescens (ATCC 13880) DNA.
ORGANISM Serratia marcescens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2672)
AUTHORS DuBose,R.F. and Hartl,D.L.
TITLE The molecular evolution of bacterial alkaline phosphatase:
Correlating variation among enteric bacteria with experimental
manipulations of the protein
JOURNAL Mol. Biol. Evol. (1990) In press
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by R.F.DuBose, 01-MAY-1990.
FEATURES from to/span description
pept 667 2094 alkaline phosphatase (phoA) (EC 3.1.3.1)
site 575 595 pho box regulatory sequence
site 2638 2672 putative VECTOR sequence M13mp18
BASE COUNT 588 a 845 c 749 g 490 t
ORIGIN
1 ggccagtgaa ttcctggcgc tgaacccgaa cggcaagatc ccggcgatca tcgatcccga
61 tggccccggc ggccggccgc tgccgctgtt cgaatccggc gccattctgc tctacctggc
121 ggaaaagagc ggccgtttcc tgccgcagga cccggcgcag cgttacgaaa ccattcaatg
181 ggtgttcttc cagatggccg ccgtcggccc gatgttcggc cagctcggtt tcttccataa
241 attcgccggg cgcgaatatg aagacaagcg gccgctggag cgctacaaaa acgaatcgaa
301 acgcctgctc ggcgtgctgg aaacgcgact tgaaggccgc gactggatca tgggcgcgga
361 gtacaccatc gccgatatct cgctgctggg ttgggtgcgc aacctgatcg gcttctatga
421 ggcgcgcgaa ctggtggagt tcgacagttt ccctcgcgtc gcgcagtggc tggagcgcgg
481 tctggcgcgc ccggcggtgc agcgtgggct cgctatcccg gcgcgcgcgg cctgagcgtc
541 aaacgggatg gccgccgggc cgtcccgcct tccgctgtca taaaattgtc atccctctgc
601 tctactctcc tttcgtaaca atttgatgtt atttacattg ttgtattacg acaaggagct
661 taccccatgc aacctgctgt atccctgatc gccggcgccg tactgtccgc cctgctctgc
721 tcctccgcca tcgcggcgga aacctcggcc aatgccgatg gcctgaccga tcgcgccgcg
781 cgcggcaacc tcgtcgaacc gggcggcgcc cgccgtctgg ccggcgatca gaccaccgcg
841 ctgaaggcat cgctgtcgga caaaacggcg aaaaacgtga tcctgctgat cggcgacggc
901 atgggggatt ccgagatcac cgccgcgcgc aactacgccg aaggcgcagg cggttacttc
961 aagggcatcg acgccctgcc gctgaccgga caatacaccc attactcgct ggacaggaaa
1021 acccacaagc cggactacgt gaccgactcc gccgcctccg ccaccgcctg ggccaccggg
1081 gtgaaaacct ataacggcgc gctcggcgtg gacgtgaacg gcaaggatca accgacgctg
1141 ctggagatcg ccaaggcggc cggcaaggcc accggcaacg tctccaccgc cgagttgcaa
1201 gacgccacgc cggcggcgct ggtctcgcac gtcatctcac gcaagtgcta tggcccggag
1261 gaaaccagcg agaaatgcgc cgctaacgcg ctggaaaatg gcggccgtgg ctccatcacc
1321 gaacaactgc tgaaaacccg cgccgacgtc acgctgggcg gcggcgcgaa atcgttcaac
1381 cagctggcaa aaagcggcga atggcaagga aaatcgctga aggaccaggc ggcggcgcag
1441 ggttatcagt gggtgagcaa cgccgacgaa ctgcaggccg ttaccctcgc caaccagcaa
1501 aaaccgctgc tggggctgtt cgccgacggc aatatgccgg tgcgttggct ggggccgaaa
1561 gccagctacc atggcaacct cgacaaaccg gcagtgacct gcgaaaacaa cccggcgcgc
1621 accgccgcca cgccaacgct ggcggcgatg accgaaaaag cgatcgcgct gttgaaagac
1681 aacccgaacg gcttcttcct gcaggtagag ggcgcgtcga tcgataaaca ggatcacgcc
1741 gccaacccgt gcgggcagat tggcgaaacc gtcgatctgg acgaagcggt acaaaaagcg
1801 ctggccttcg cccgcgccga cggcaacacg ctggtgatcg tcaccgccga ccacgcgcac
1861 gccagccaga tcgtctccgc cgacgccaaa gcgccgggcc tgacccaaaa gctgaccacc
1921 aaggacggcg cgccgatgac cctcagctac ggcaactctg aagaggaatc tcagggacac
1981 accggcaccc agctgcgcgt cgcggcctac ggcccgcacg ccgccaacgt agtgggcctg
2041 accgatcaga ccgatctgtt cttcaccatg cgcgatgcga tgggtatcaa gtaaccgacc
2101 cctgaacgat ggccgcccgc cacggcggcc atttattatt ggttaccgct aggtatttgc
2161 aatttaaccg caattattgt gtgataacga aataacgtct aattacgcgg caattaaccc
2221 atgaaaataa cgcggttata cggcctttct tccctctctg tttattcctg cgacagcaac
2281 cggccgtttt ttgcttatat tcattttttt tagttacaaa agtagttttt aacattctcg
2341 caaacaataa taccccgcca gtaacgtgaa ccgatcaatt tgcatccgga cggcctgcaa
2401 tagttcactt ttcgttaaat accctattaa caaaaaggca ttattgctgc ctatcggctt
2461 tcgccgggcg gtcggcttca ttatttccag ggtaactccc gctaaattct ttcacctgca
2521 atgagtagaa tttacacaca agagatttaa ttcaatgaga aaattactcg tcctgatttt
2581 cagtctcagc ctgtttggcg ttacgcaaca ggcggcggct gaagagaaga ataaaaccgt
2641 cgacctgcag gcatgcaagc ttggcactgg cc
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