GenBank-Updates@genbank.bio.net (10/31/90)
LOCUS VAC415 4914 bp ds-DNA VRL 31-OCT-1990 DEFINITION Vaccinia virus DNA-dependent RNA polymerase-subunit. ACCESSION M37415 KEYWORDS DNA-dependent RNA polymerase-subunit rpo132. SOURCE Vaccinia virus (strain WR) DNA. ORGANISM Vaccinia virus Viridae; ds-DNA enveloped viruses; Poxviridae; Orthopoxvirus. REFERENCE 1 (bases 1 to 4914) AUTHORS Amegadzie,B.Y., Holmes,M.H., Cole,N.B., Jones,E.V., Earl,P.L. and Moss,B. TITLE Identification, sequence, and expression of the gene encoding the second-largest subunit of the vaccinia virus DNA-dependent RNA polymerase JOURNAL Unpublished (1990) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence for [1] kindly submitted by B.Y.Amegadzie, 03-AUG-1990. Author address: B.Y.Amegadzie NIAID, Lab of Viral Diseases, 9000 Rockville Pike, Bethesda, MD 20892 USA FEATURES from to/span description pept 455 3949 RNA polymerase-subunit rpo132 pept 4583 3954 (c) ORF BASE COUNT 1598 a 863 c 940 g 1513 t ORIGIN 1 gtatacacat gattgacaaa ttattctatg tctattataa tttatttctg ttgttcgaag 61 atatcatcca aaacgagtac tttaaagaag tagctaatgt tgtaaaccac gtacttacgg 121 ctacggcatt agatgagaaa ttattcctaa ttaagaaaat ggctgaacac gatgtttatg 181 gagttagcaa tttcaaaata gggatgttta acctgacatt tattaagtcg ttggatcata 241 ccgttttccc ctctctgtta gatgaggata gcaaaataaa gttttttaag gggaaaaagc 301 tcaatattgt agcattacga tctctggagg attgtataaa ttacgtgact aaatccgaga 361 atatgataga aatgatgaag gaaagatcga ctattttaaa tagcatagat atagaaacgg 421 aatcggtaga tcgtctaaaa gaattgcttc taaaatgaaa aaaaacactg attcagaaat 481 ggatcaacga ctagggtata agtttttggt gcctgatcct aaagccggag ttttttatag 541 accgttacat ttccaatatg tatcgtattc taattttata ttgcatcgat tgcatgaaat 601 cttgaccgtc aagcggccac tcttatcgtt taagaataat acagaacgaa ttatgataga 661 aattagcaat gttaaagtga ctcctccaga ttactcacct ataatcgcga gtattaaagg 721 taagagttat gatgcattag ccacgttcac tgtaaatatc tttaaagagg taatgaccaa 781 agagggtata tccatcacta aaataagtag ttatgaggga aaagattctc atttgataaa 841 aattccgcta ctaataggat acgggaataa aaatccactt gatacagcca agtatcttgt 901 tcctaatgtc ataggtggag tctttatcaa taaacaatct gtcgaaaaag taggaattaa 961 tctagtagaa aagattacaa catggccaaa atttagggtt gttaagccaa actcattcac 1021 tttctcgttt tcctccgtat cccctcctaa tgtattaccg acaagatatc gccattacaa 1081 gatatctctg gatatatcac aattggaagc gttgaatata tcatcgacaa agacatttat 1141 aacggtcaat attgttttgc tgtctcaata tttatctaga gtgagtctag aattcattag 1201 acgtagttta tcatacgata tgcctccaga agttgtctat ctagtaaacg cgataataga 1261 tagtgctaaa cgaattactg aatctattac tgactttaat attgatacat acattaatga 1321 cctggtggaa gctgaacaca ttaaacaaaa atctcagtta acgatcaacg agttcaaata 1381 tgaaatgctg cataactttt tacctcatat gaactataca cccgatcaac taaagggatt 1441 ttatatgata tctttactaa gaaagtttct ctactgtatc taccacactt ctagatatcc 1501 agatagagat tcgatggttt gtcatcgcat cctaacgtac ggcaaatatt ttgagacgtt 1561 ggcacatgat gaattagaga attacatagg caacatccga aacgatatca tgaacaatca 1621 caagaacaga ggcacttacg cggtaaacat tcatgtacta acaactcccg gacttaatca 1681 cgcgttttct agcttattga gtggaaagtt caaaaagtca gacggtagtt atcgaacaca 1741 tcctcactat tcatggatgc agaatatttc tattcctagg agtgttggat tttatccgga 1801 tcaagtaaag atttcaaaga tgttttctgt cagaaaatac catccaagtc aatatcttta 1861 cttttgttca tcggacgttc cggaaagagg tcctcaggta ggtttagtat ctcaattgtc 1921 tgtcttgagt tccattacaa atatactaac gtctgagtat ttggatttgg aaaagaaaat 1981 ttgtgagtat atcagatcat attataaaga tgatataagt tactttgaaa caggatttcc 2041 aatcactata gaaaatgctc tagtcgcatc tcttaatcca aatatgatat gtgattttgt 2101 aactgacttt agacgtagaa aacggatggg attcttcggt aacttggagg taggtattac 2161 tttagttagg gatcacatga atgaaattcg cattaatatt ggagcgggaa gattagtcag 2221 accattcttg gttgtggata acggagagct catgatggat gtgtgtccgg agttagaaag 2281 cagattagac gacatgacat tctctgacat tcagaaagag tttccgcatg tcatcgaaat 2341 ggtagatata gaacaattta cttttagtaa cgtatgtgaa tcggttcaaa aatttagaat 2401 gatgtcaaag gatgaaagaa agcaatacga tttatgtgac tttcctgccg aatttagaga 2461 tggatatgtg gcatcttcat tagtgggaat caatcacaat tctggaccca gagctattct 2521 tggatgtgct caagctaaac aagctatctc ttgtctgagt tcggatatac gaaataaaat 2581 agacaatgga attcatttga tgtatccaga gaggccaatc gtgattagta aggctttaga 2641 aacttcaaag attgcggcta attgcttcgg ccaacatgtt actatagcat taatgtcgta 2701 caaaggtatc aatcaagagg atggaattat catcaaaaaa caatttattc agagaggcgg 2761 tctcgatata gttaccgcaa agaaacatca agtagaaatt ccgttggaaa actttaataa 2821 caaagaaaga gataggtcta acgcctattc aaaattagaa agtaatggat tagttagact 2881 gaatgctttc ttggaatccg gagacgctat ggcacgaaat atctcatcaa gaactcttga 2941 agatgatttt gctagagata atcagattag cttcgatgtt tccgagaaat ataccgatat 3001 gtacaaatct cgcgttgaac gagtacaagt agaacttact gacaaagtta aggtacgagt 3061 attaaccatg aaagaaagaa gacccattct aggagataaa tttaccacta gaacgagtca 3121 aaagggaaca gtcgcgtatg tcgcggatga aacggaactt ccatacgacg aaaatggtat 3181 cacaccagat gtcattatta attctacatc catcttctct agaaaaacta tatctatgtt 3241 gatagaggtt attttaacag ccgcatattc tgctaagccg tacaacaata agggagaaaa 3301 ccgacctgtc tgttttccta gtagtaacga aacatccatc gatacatata tgcaattcgc 3361 taaacaatgt tatgagcatt caaatccgaa attgtccgat gaagaattat cggataaaat 3421 cttttgtgaa aagattctct atgatcctga aacggataag ccttatgcat ccaaagtatt 3481 ttttggacca atttattact tgcgtctgag gcatttaact caggacaagg caaccgttag 3541 atgtagaggt aaaaagacga agctcattag acaggcgaat gagggacgaa aacgtggagg 3601 aggtatcaag ttcggagaaa tggagagaga ctgtttaata gcgcatggcg cagccaatac 3661 tattacagaa gttttaaaag actcagaaga ggattatcaa gatgtgtatg tttgtgaaaa 3721 ttgtggagac atagcagcac aaatcaaggg tattaataca tgtcttagat gttcaaaact 3781 taatctctct cctctcttaa caaaaattga taccacgcac gtatctaaag tatttcttac 3841 tcaaatgaac gccagaggcg taaaagtcaa attagatttc gaacgaaggc ctccttcgtt 3901 ttataaacca ttagataaag ttgatctcaa gccgtctttt ctggtgtaat attctagttt 3961 ggtagtagat acatatcaat atcatcaaat tcgagatccg aattataaaa tgggcgtgga 4021 ttgttaacta tagaatcgga cgtctgatat tcgaaaatct gtggagtttt aggttttggt 4081 ggaggtgtaa ctgctacttg ggatactgaa gtctgatatt cagaaagctg ggggatgttc 4141 tggttcgaca tccacgatgg tgtcacatca ctaatcggtt cggtaacgtc tgtggatgga 4201 ggtgctactt ctacagaacc tgtagcctca gttgtcaacg gagatacatt tttaatgcga 4261 ggaaatgtat aatttggtaa tggtttctca tgtggatctg aagaagaggt aagatatcta 4321 ctagaaagat accgatcacg ttctagttct cttttgtaga acttaacttt ttctttctca 4381 gcatctagtt gatattccaa cctcttcacg ttactacgtt cagattccaa ttcacgttcg 4441 catgggttac ctccgcagtt tttacgagcg atttcacgtt cagccttcat gcgtctctcc 4501 ttctctctat cgagtttatc agagcagtct ttctgaaggc gatcgaactc cataaatttc 4561 tccaacgctt tgattgtttc catagatttc cgaagttcag cttttaggac tgtgattctt 4621 tttctttcga attcacagct ggatgtgcaa ccgtttccat taccgccatc tctaagtttc 4681 ttttctagat cggcaacatt tcatccccat gccttttaca ttcctcgagt ctactgtcgt 4741 cgaaatatcg ttccagctcc ttttcgacat caataacttt agcacgttgt ctctcaagct 4801 ctcttttgta gttatctgat tccctggcac gtttaagatc ttcatgcaat tgagtcagct 4861 cttaacacaa tctcttgctt cttcgtcata gtacttacaa tcactatggg atcc //
GenBank-Updates@genbank.bio.net (10/31/90)
LOCUS VACRPO132 4914 bp ds-DNA VRL 31-OCT-1990 DEFINITION Vaccinia virus DNA-dependent RNA polymerase-subunit. ACCESSION M37415 KEYWORDS DNA-dependent RNA polymerase-subunit rpo132. SOURCE Vaccinia virus (strain WR) DNA. ORGANISM Vaccinia virus Viridae; ds-DNA enveloped viruses; Poxviridae; Orthopoxvirus. REFERENCE 1 (bases 1 to 4914) AUTHORS Amegadzie,B.Y., Holmes,M.H., Cole,N.B., Jones,E.V., Earl,P.L. and Moss,B. TITLE Identification, sequence, and expression of the gene encoding the second-largest subunit of the vaccinia virus DNA-dependent RNA polymerase JOURNAL Unpublished (1990) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence for [1] kindly submitted by B.Y.Amegadzie, 03-AUG-1990. Author address: B.Y.Amegadzie NIAID, Lab of Viral Diseases, 9000 Rockville Pike, Bethesda, MD 20892 USA FEATURES from to/span description pept 455 3949 RNA polymerase-subunit rpo132 pept 4583 3954 (c) ORF BASE COUNT 1598 a 863 c 940 g 1513 t ORIGIN 1 gtatacacat gattgacaaa ttattctatg tctattataa tttatttctg ttgttcgaag 61 atatcatcca aaacgagtac tttaaagaag tagctaatgt tgtaaaccac gtacttacgg 121 ctacggcatt agatgagaaa ttattcctaa ttaagaaaat ggctgaacac gatgtttatg 181 gagttagcaa tttcaaaata gggatgttta acctgacatt tattaagtcg ttggatcata 241 ccgttttccc ctctctgtta gatgaggata gcaaaataaa gttttttaag gggaaaaagc 301 tcaatattgt agcattacga tctctggagg attgtataaa ttacgtgact aaatccgaga 361 atatgataga aatgatgaag gaaagatcga ctattttaaa tagcatagat atagaaacgg 421 aatcggtaga tcgtctaaaa gaattgcttc taaaatgaaa aaaaacactg attcagaaat 481 ggatcaacga ctagggtata agtttttggt gcctgatcct aaagccggag ttttttatag 541 accgttacat ttccaatatg tatcgtattc taattttata ttgcatcgat tgcatgaaat 601 cttgaccgtc aagcggccac tcttatcgtt taagaataat acagaacgaa ttatgataga 661 aattagcaat gttaaagtga ctcctccaga ttactcacct ataatcgcga gtattaaagg 721 taagagttat gatgcattag ccacgttcac tgtaaatatc tttaaagagg taatgaccaa 781 agagggtata tccatcacta aaataagtag ttatgaggga aaagattctc atttgataaa 841 aattccgcta ctaataggat acgggaataa aaatccactt gatacagcca agtatcttgt 901 tcctaatgtc ataggtggag tctttatcaa taaacaatct gtcgaaaaag taggaattaa 961 tctagtagaa aagattacaa catggccaaa atttagggtt gttaagccaa actcattcac 1021 tttctcgttt tcctccgtat cccctcctaa tgtattaccg acaagatatc gccattacaa 1081 gatatctctg gatatatcac aattggaagc gttgaatata tcatcgacaa agacatttat 1141 aacggtcaat attgttttgc tgtctcaata tttatctaga gtgagtctag aattcattag 1201 acgtagttta tcatacgata tgcctccaga agttgtctat ctagtaaacg cgataataga 1261 tagtgctaaa cgaattactg aatctattac tgactttaat attgatacat acattaatga 1321 cctggtggaa gctgaacaca ttaaacaaaa atctcagtta acgatcaacg agttcaaata 1381 tgaaatgctg cataactttt tacctcatat gaactataca cccgatcaac taaagggatt 1441 ttatatgata tctttactaa gaaagtttct ctactgtatc taccacactt ctagatatcc 1501 agatagagat tcgatggttt gtcatcgcat cctaacgtac ggcaaatatt ttgagacgtt 1561 ggcacatgat gaattagaga attacatagg caacatccga aacgatatca tgaacaatca 1621 caagaacaga ggcacttacg cggtaaacat tcatgtacta acaactcccg gacttaatca 1681 cgcgttttct agcttattga gtggaaagtt caaaaagtca gacggtagtt atcgaacaca 1741 tcctcactat tcatggatgc agaatatttc tattcctagg agtgttggat tttatccgga 1801 tcaagtaaag atttcaaaga tgttttctgt cagaaaatac catccaagtc aatatcttta 1861 cttttgttca tcggacgttc cggaaagagg tcctcaggta ggtttagtat ctcaattgtc 1921 tgtcttgagt tccattacaa atatactaac gtctgagtat ttggatttgg aaaagaaaat 1981 ttgtgagtat atcagatcat attataaaga tgatataagt tactttgaaa caggatttcc 2041 aatcactata gaaaatgctc tagtcgcatc tcttaatcca aatatgatat gtgattttgt 2101 aactgacttt agacgtagaa aacggatggg attcttcggt aacttggagg taggtattac 2161 tttagttagg gatcacatga atgaaattcg cattaatatt ggagcgggaa gattagtcag 2221 accattcttg gttgtggata acggagagct catgatggat gtgtgtccgg agttagaaag 2281 cagattagac gacatgacat tctctgacat tcagaaagag tttccgcatg tcatcgaaat 2341 ggtagatata gaacaattta cttttagtaa cgtatgtgaa tcggttcaaa aatttagaat 2401 gatgtcaaag gatgaaagaa agcaatacga tttatgtgac tttcctgccg aatttagaga 2461 tggatatgtg gcatcttcat tagtgggaat caatcacaat tctggaccca gagctattct 2521 tggatgtgct caagctaaac aagctatctc ttgtctgagt tcggatatac gaaataaaat 2581 agacaatgga attcatttga tgtatccaga gaggccaatc gtgattagta aggctttaga 2641 aacttcaaag attgcggcta attgcttcgg ccaacatgtt actatagcat taatgtcgta 2701 caaaggtatc aatcaagagg atggaattat catcaaaaaa caatttattc agagaggcgg 2761 tctcgatata gttaccgcaa agaaacatca agtagaaatt ccgttggaaa actttaataa 2821 caaagaaaga gataggtcta acgcctattc aaaattagaa agtaatggat tagttagact 2881 gaatgctttc ttggaatccg gagacgctat ggcacgaaat atctcatcaa gaactcttga 2941 agatgatttt gctagagata atcagattag cttcgatgtt tccgagaaat ataccgatat 3001 gtacaaatct cgcgttgaac gagtacaagt agaacttact gacaaagtta aggtacgagt 3061 attaaccatg aaagaaagaa gacccattct aggagataaa tttaccacta gaacgagtca 3121 aaagggaaca gtcgcgtatg tcgcggatga aacggaactt ccatacgacg aaaatggtat 3181 cacaccagat gtcattatta attctacatc catcttctct agaaaaacta tatctatgtt 3241 gatagaggtt attttaacag ccgcatattc tgctaagccg tacaacaata agggagaaaa 3301 ccgacctgtc tgttttccta gtagtaacga aacatccatc gatacatata tgcaattcgc 3361 taaacaatgt tatgagcatt caaatccgaa attgtccgat gaagaattat cggataaaat 3421 cttttgtgaa aagattctct atgatcctga aacggataag ccttatgcat ccaaagtatt 3481 ttttggacca atttattact tgcgtctgag gcatttaact caggacaagg caaccgttag 3541 atgtagaggt aaaaagacga agctcattag acaggcgaat gagggacgaa aacgtggagg 3601 aggtatcaag ttcggagaaa tggagagaga ctgtttaata gcgcatggcg cagccaatac 3661 tattacagaa gttttaaaag actcagaaga ggattatcaa gatgtgtatg tttgtgaaaa 3721 ttgtggagac atagcagcac aaatcaaggg tattaataca tgtcttagat gttcaaaact 3781 taatctctct cctctcttaa caaaaattga taccacgcac gtatctaaag tatttcttac 3841 tcaaatgaac gccagaggcg taaaagtcaa attagatttc gaacgaaggc ctccttcgtt 3901 ttataaacca ttagataaag ttgatctcaa gccgtctttt ctggtgtaat attctagttt 3961 ggtagtagat acatatcaat atcatcaaat tcgagatccg aattataaaa tgggcgtgga 4021 ttgttaacta tagaatcgga cgtctgatat tcgaaaatct gtggagtttt aggttttggt 4081 ggaggtgtaa ctgctacttg ggatactgaa gtctgatatt cagaaagctg ggggatgttc 4141 tggttcgaca tccacgatgg tgtcacatca ctaatcggtt cggtaacgtc tgtggatgga 4201 ggtgctactt ctacagaacc tgtagcctca gttgtcaacg gagatacatt tttaatgcga 4261 ggaaatgtat aatttggtaa tggtttctca tgtggatctg aagaagaggt aagatatcta 4321 ctagaaagat accgatcacg ttctagttct cttttgtaga acttaacttt ttctttctca 4381 gcatctagtt gatattccaa cctcttcacg ttactacgtt cagattccaa ttcacgttcg 4441 catgggttac ctccgcagtt tttacgagcg atttcacgtt cagccttcat gcgtctctcc 4501 ttctctctat cgagtttatc agagcagtct ttctgaaggc gatcgaactc cataaatttc 4561 tccaacgctt tgattgtttc catagatttc cgaagttcag cttttaggac tgtgattctt 4621 tttctttcga attcacagct ggatgtgcaa ccgtttccat taccgccatc tctaagtttc 4681 ttttctagat cggcaacatt tcatccccat gccttttaca ttcctcgagt ctactgtcgt 4741 cgaaatatcg ttccagctcc ttttcgacat caataacttt agcacgttgt ctctcaagct 4801 ctcttttgta gttatctgat tccctggcac gtttaagatc ttcatgcaat tgagtcagct 4861 cttaacacaa tctcttgctt cttcgtcata gtacttacaa tcactatggg atcc //