GenBank-Updates@genbank.bio.net (11/01/90)
LOCUS BSTAMYABS 1891 bp ds-DNA BCT 01-NOV-1990
DEFINITION B.stearothermophilus alpha amylase gene, complete cds.
ACCESSION M57457
KEYWORDS alpha amylase.
SOURCE B.stearothermophilus (ATCC 12980) DNA.
ORGANISM Bacillus stearothermophilus
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 1891)
AUTHORS Suominen,I., Karp,M., Lautamo,J., Knowles,J. and Mantsaelae,P.
TITLE Thermostable alpha amylase of Bacillus stearothermophilus: Cloning,
expression, and secretion by Escherichia coli
JOURNAL Extracellular Enzymes Microorganisms 0, 129-137 (1987)
STANDARD simple staff_entry
FEATURES from to/span description
pept 235 1884 alpha-amylase precursor
sigp 235 336 alpha-amylase signal peptide
matp 337 1881 alpha-amylase
signal 89 94 -35 region P1
signal 114 119 -10 region P1
signal 185 190 -35 region P2 promoter
signal 207 212 -10 region P2
BASE COUNT 498 a 469 c 464 g 460 t
ORIGIN
1 gatcatattt tgcatcggtc ctggccgtca tccaccctac tgcccgatca cccgcacggt
61 gaaagtcacc aatgtgggac gtccgtcgat ggcagaagat cacaaataaa aattataata
121 gacgtaaccg ttcgaggttt tgcttcctgt ttactctttt tatgcaatca tttcccttca
181 ttttttggaa tccaaaccgt cgaatgtaac atttgattaa gggggaaggg cattgtgcta
241 acgtttcacc gcatcattcg aaaaggatgg atgttcctgc tcgcgttttt gctcactgcc
301 ttgctgttct gcccaaccgg acagcccgcc aaggctgccg caccgtttaa cggcaccatg
361 atgcagtatt ttgaatggta cttgccggat gatggcacgt tatggaccaa agtggccaat
421 gaagccaaca acttatccag ccttggcatc accgctcttt ggctgccgcc cgcttataaa
481 ggaacaagcc gcagcgacgt agggtacgga gtatacgact tgtatgacct cggtgaattc
541 aatcaaaaag gggccgtccg cacaaaatac ggaacaaaag ctcaatatct tcaagccatt
601 caagccgccc acgccgctgg aatgcaagtg tacgccgatg tcgtgttcga ccataaaggc
661 ggcgccgacg gcacggaatg ggtggacgcc gtcgaagtca atccgtccga ccgcaaccaa
721 gaaatctcgg gcatctatca aatccaagca tggacgaaat ttgattttcc cgggcggggc
781 aacacctact ccagctttaa gtggcgctgg taccattttg atggcgttga ttgggacgaa
841 agccgaaaat tgagccgcat ttacaaattc cgcggcatcg gcaaagcgtg ggattgggaa
901 gtagacacgg aaaacggaaa ctatgactac ttaatgtatg ccgaccttga tatggatcat
961 cccgaagtcg tgactgagct aaaagctggg gctaaatggt atgtcaacac aagaacactt
1021 gatgggttcc ggcttgatgc cgtcaagcat attaagttca gtttttttcc tgattggttg
1081 tcgtatgtgc gttctcagac tggcaagccg ctatttaccg ttggggaata ttggagctat
1141 gacatcaaca agttgcacaa ttacattatg aaaacaaacg gaacgatgtc tttgtttgat
1201 gccccgttac acaacaaatt ttataccgct tccaaatcag ggggcacatt tgatatgcgc
1261 acgttaatga ccaatactct catgaaagat caaccaacat tggccgtcac cttcgttgat
1321 aatcatgaca ccgaacccgg ccaagcgctg cagtcatggg tcgacccatg gttcaaaccg
1381 ttggcttacg cctttattct aactcggcag gaaggatacc cgtgcgtctt ttatggtgac
1441 tattatggca ttccacaata taacattcct tcgctgaaaa gcaaaatcga tccgctcctc
1501 atcgcgcgca gggattatgc ttacggaacg caacatgatt atcttgatca ctccgacatc
1561 atcgggtgga caagggaagg ggtcactgaa aaaccaggat ccggactggc cgcattgatc
1621 accgatgggc cgggaggaag caaatggatg tacgttggca aacaacacgc cggaaaagtg
1681 ttctatgacc ttaccggcaa ccggagtgac accgtcacca tcaacagtga tggatggggg
1741 gaattcaaag tcaatggcgg ttcggtttcg gtttgggttc ctagaaaaac gaccgtctct
1801 actatcgctt ggtcgatcac aacccgaccg tggactgatg aattcgtccg ttggaccgaa
1861 ccacggttgg tggcatggcc ttgatgcctg c
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