[bionet.molbio.genbank.updates] E.coli Leu-tRNA-1.

GenBank-Updates@genbank.bio.net (11/06/90)

LOCUS       ECOTRL1        87 bp ss-tRNA            RNA       06-NOV-1990
DEFINITION  E.coli Leu-tRNA-1.
ACCESSION   K01550
KEYWORDS    transfer RNA; transfer RNA-Leu.
SOURCE      Escherichia coli strains B and K12 RNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria; 
            Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 87)
  AUTHORS   Dube,S.K., Marcker,K.A. and Yudelevich,A.
  TITLE     The nucleotide sequence of a leucine transfer RNA from E. coli
  JOURNAL   FEBS Lett. 9, 168-170 (1970)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 87)
  AUTHORS   Blank,H.-U. and Soell,D.
  TITLE     The nucleotide sequence of two leucine tRNA species from
            Escherichia coli K12
  JOURNAL   Biochem. Biophys. Res. Commun. 43, 1192-1197 (1971)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Yudelevich,A.
  TITLE     modification of Leucine tRNA of Escherichia coli after
            bacteriophage T4 infection
  JOURNAL   Basic Life Sci. 1, 229-239 (1973)
  STANDARD  full staff_review
COMMENT     Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
            entry 1010 in Nucleic Acids Res. 11, r1-r54 (1983). [1] suggests
            that the unidentified guanosine derivative at 38 is either
            1'-O-methylguanosine or 2'-O-methylguanosine. [2] also sequenced
            E.coli Leu-tRNA-2.
FEATURES       from  to/span     description
    tRNA          1       87     Leu-tRNA-1 (NAR: 1010)
                                 /anticodon="(pos:35..37,aa:Leu)"
    modified     16       16     d = dihydrouridine
    modified     17       17     d = dihydrouridine
    modified     18       18     gm = 2'-O-methylguanosine
    modified     20       20     d = dihydrouridine
    modified     38       38     unidentified guanosine derivative (see
                                 comment)
    modified     39       39     f = pseudouridine
    modified     41       41     f = pseudouridine
    modified     65       65     t = 5-methyluridine
    modified     66       66     f = pseudouridine
BASE COUNT       15 a     24 c     29 g     19 t
ORIGIN      5' end of mature tRNA.
        1 gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg
       61 ggggttcaag tcccccccct cgcacca
//