GenBank-Updates@genbank.bio.net (11/08/90)
LOCUS AFAPHBAA 2768 bp ds-DNA BCT 08-NOV-1990
DEFINITION A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene, complete cds
and poly-beta-hydroxybuterate-A (phbA) gene, 5' end.
ACCESSION J05003
KEYWORDS poly-beta-hydroxybuterase polymerase.
SOURCE A.eutrophus (strain H16) DNA.
ORGANISM Alcaligenes eutrophus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Alcaligenaceae.
REFERENCE 1 (bases 1 to 2768)
AUTHORS Peoples,O.P. and Sinskey,A.J.
TITLE Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes
eutrophus H16: Identification and characterization of the PHB
polymerase gene
JOURNAL J. Biol. Chem. 264, 15298-15303 (1989)
STANDARD simple staff_review
FEATURES from to/span description
pept 842 2611 poly-beta-hydroxybuterate-C (put.)
pept 2696 > 2768 poly-beta-hydroxybuterate-A
BASE COUNT 488 a 927 c 902 g 451 t
ORIGIN
1 cccgggcaag taccttgccg acatctatgc gctggcgcgc acgcgcctgg cgcgcgccgg
61 ctgtaccgag gtctacggcg gcgacgcctg caccgtggcc gacgccggtc gcttctactc
121 ctatcggcgc gatggcgtga ccggccgcat ggccagcctg gtctggctgg cggactgagc
181 ccgccgctgc ctcactcgtc cttgcccctg gccgcctgcg cgcgctcggc ttcagccttg
241 cgtcggcggc ggccgggcgt gcccatgatg tagagcacca cgccaccggc gccatgccat
301 acatcaggaa ggtggcaacg cctgccacca cgttgtgctc ggtgatcgcc atcatcagcg
361 ccacgtagag ccagccaatg gccacgatgt acatcaaaaa ttcatccttc tcgcctatgc
421 tctggggcct cggcagatgc gagcgctgca taccgtccgg taggtcggga agcgtgcagt
481 gccgaggcgg attcccgcat tgacagcgcg tgcgttgcaa ggcaacaatg gactcaaatg
541 tctcggaatc gctgacgatt cccaggtttc tccggcaagc atagcgcatg gcgtctccat
601 gcgagaatgt cgcgcttgcc ggataaaagg ggagccgcta tcggaatgga cgcaagccac
661 ggccgcagca ggtgcggtcg agggcttcca gccagttcca gggcagatgt gccggcagac
721 cctcccgctt tgggggaggc gcaagccggg tccattcgga tagcatctcc ccatgcaaag
781 tgccggccag ggcaatgccc ggagccggtt cgaatagtga cggcagagag acaatcaaat
841 catggcgacc ggcaaaggcg cggcagcttc cacgcaggaa ggcaagtccc aaccattcaa
901 ggtcacgccg gggccattcg atccagccac atggctggaa tggtcccgcc agtggcaggg
961 cactgaaggc aacggccacg cggccgcgtc cggcattccg ggcctggatg cgctggcagg
1021 cgtcaagatc gcgccggcgc agctgggtga tatccagcag cgctacatga aggacttctc
1081 agcgctgtgg caggccatgg ccgagggcaa ggccgaggcc accggtccgc tgcacgaccg
1141 gcgcttcgcc ggcgacgcat ggcgcaccaa cctcccatat cgcttcgctg ccgcgttcta
1201 cctgctcaat gcgcgcgcct tgaccgagct ggccgatgcc gtcgaggccg atgccaagac
1261 ccgccagcgc atccgcttcg cgatctcgca atgggtcgat gcgatgtcgc ccgccaactt
1321 ccttgccacc aatcccgagg cgcagcgcct gctgatcgag tcgggcggcg aatcgctgcg
1381 tgccggcgtg cgcaacatga tggaagacct gacacgcggc aagatctcgc agaccgacga
1441 gagcgcgttt gaggtcggcc gcaatgtcgc ggtgaccgaa ggcgccgtgg tcttcgagaa
1501 cgagtacttc cagctgttgc agtacaagcc gctgaccgac aaggtgcacg cgcgcccgct
1561 gctgatggtg ccgccgtgca tcaacaagta ctacatcctg gacctgcagc cggagagctc
1621 gctggtgcgc catgtggtgg agcagggaca tacggtgttt ctggtgtcgt ggcgcaatcc
1681 ggacgccagc atggccggca gcacctggga cgactacatc gagcacgcgg ccatccgcgc
1741 catcgaagtc gcgcgcgaca tcagcggcca ggacaagatc aacgtgctcg gcttctgcgt
1801 gggcggcacc attgtctcga ccgcgctggc ggtgctggcc gcgcgcggcg agcacccggc
1861 cgccagcgtc acgctgctga ccacgctgct ggactttgcc gacacgggca tcctcgacgt
1921 ctttgtcgac gagggccatg tgcagttgcg cgaggccacg ctgggcggcg gcgccggcgc
1981 gccgtgcgcg ctgctgcgcg gccttgagct ggccaatacc ttctcgttct tgcgcccgaa
2041 cgacctggtg tggaactacg tggtcgacaa ctacctgaag ggcaacacgc cggtgccgtt
2101 cgacctgctg ttctggaacg gcgacgccac caacctgccg gggccgtggt actgctggta
2161 cctgcgccac acctacctgc agaacgagct caaggtaccg ggcaagctga ccgtgtgcgg
2221 cgtgccggtg gacctggcca gcatcgacgt gccgacctat atctacggct cgcgcgaaga
2281 ccatatcgtg ccgtggaccg cggcctatgc ctcgaccgcg ctgctggcga acaagctgcg
2341 cttcgtgctg ggtgcgtcgg gccatatcgc cggtgtgatc aacccgccgg ccaagaacaa
2401 gcgcagccac tggactaacg atgcgctgcc ggagtcgccg cagcaatggc tggccggcgc
2461 catcgagcat cacggcagct ggtggccgga ctggaccgca tggctggccg ggcaggccgg
2521 cgcgaaacgc gccgcgcccg ccaactatgg caatgcgcgc tatcgcgcaa tcgaacccgc
2581 gcctgggcga tacgtcaaag ccaaggcatg acgcttgcat gagtgccggc gtgcgtcatg
2641 cacggcgccg gcaggcctgc aggttccctc ccgtttccat tgaaaggact acacaatgac
2701 tgacgttgtc atcgtatccg ccgcccgcac cgcggtcggc aagtttggcg gctcgctggc
2761 caagatcc
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