GenBank-Updates@genbank.bio.net (11/08/90)
LOCUS PECPCB 12364 bp ds-DNA PLN 08-NOV-1990
DEFINITION P.chrysogenum alpha-aminoadypil-cysteinyl-valine synthetase (pcbAB)
gene, complete cds.
ACCESSION M57425 J05604
KEYWORDS alpha-aminoadipyl-cysteinyl-valine synthetase.
SOURCE P.chryosogenum (strain ASP-78) mycellium DNA, clone 1A and 6A.
ORGANISM Penicillium chrysogenum
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 12364)
AUTHORS Diez,B., Gutierrez,S., Barredo,J.L., van Solingen,P.,
van der Voort,L.H.M. and Martin,J.F.
TITLE The cluster of penicillin biosynthetic genes: Identification and
characterization of the pcbAB gene encoding the
alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the
pcbC and penDE genes
JOURNAL J. Biol. Chem. 265, 16358-16365 (1990)
STANDARD simple staff_entry
FEATURES from to/span description
pept 265 11640 alpha-aminoadipyl-cysteinyl-valine synthetase
(pcbAB)
signal 128 133 TATA box
signal 167 171 TATA box
BASE COUNT 2958 a 3386 c 3236 g 2783 t 1 others
ORIGIN
1 dgtcgacagt tgacagagcc aatggcatcg gatctgccct agaccgtgct agacgaaagt
61 ctccatcttg tctgcgggca gtgcttcagt cgcccagatt ctcgatggag attggccagg
121 tcagccatat ataccctgca atggcagacc aatgcagcag gcccagtata aggaattccc
181 ctcgagcttg tctgtgattg cgttttttct aacacttgtt gttgcatccg atccgtccct
241 accaattatt ggtcattgac agacatgact caactgaagc caccgaacgg aaccacgccg
301 ataggcttct cggccactac atccctgaac gccagtggga gctcgagtgt gaaaaatggg
361 accatcaaac ccagcaatgg catcttcaag cccagcacta gggacaccat ggacccttgc
421 agtgggaatg cggccgatgg cagtatccgc gtccgtttcc gtggaggaat cgaacggtgg
481 aaggagtgcg tcaaccaggt ccccgagcgc tgcgacctga gtggtctgac aaccgactcc
541 acgcgatatc agctcgcatc gactgggttc ggtgacgcga gcgctgccta ccaggagcgc
601 ttgatgacgg tccctgttga cgtacatgcc gcgctccaag agctgtgcct agaacgccgt
661 gtgagcgtgg gatccgtcat taatttctcc gtgcaccaga tgctgaaagg gtttggaaat
721 ggcacacaca ctatcaccgc ctctctgcac cgtgagcaga atttgcagaa ttcttcgcca
781 tcctgggtag tctcccccac aatcgtcacc catgagaaca gagacggatg gtccgtcgcg
841 caggcggtcg agagtatcga agcggggcgc ggttccgaga aggagtcagt gactgcgatt
901 gactccgggt caagtctcgt gaaaatgggg ttatttgact tactcgtcag ctttgtcgat
961 gcagacgatg ctcgtattcc atgtttcgac tttcccctcg cagtgatagt gcgtgagtgt
1021 gatgccaacc tctcgctgac tctgcgtttc tccgactgtc tcttcaacga ggagacgata
1081 tgcaatttta ccgatgccct aaacatcttg ctcgccgaag cagtgatagg aagagtgacc
1141 ccggttgccg atatcgaact actatccgcg gagcagaagc agcagctgga agagtggaac
1201 aacacggatg gcgagtaccc ttcatcaaag cgactgcacc atctcattga agaggtggtt
1261 gaacggcatg aagacaaaat agccgttgtc tgcgacgagc gagagctcac ttacggcgag
1321 ctcaatgccc aaggcaacag cctcgcacgc tatctccgtt ccattggtat cctgcccgag
1381 cagctagtcg cattgtttct agataagagc gagaagctca ttgttaccat cctcggcgtg
1441 tggaaatccg gcgccgccta cgtgcccatc gacccgactt atccggatga gcgagtgcgc
1501 ttcgtgctgg atgacaccaa ggcacgggcc atcatcgcca gtaatcaaca tgtggagagg
1561 ctccagcgag aggtcatcgg cgatagaaac ctatgcatta tccgtctgga gcccttgttg
1621 gcctcccttg ctcaggattc ctcaaaattc cccgcgcata acttggacga cctacccctc
1681 acaagccagc agctcgccta tgtgacttac acctctggga ccactggctt cccaaagggc
1741 atatttaaac aacacaccaa tgtggtgaac agtatcaccg acctgtctgc aaggtacggg
1801 gtggccgggc agcaccacga agccattctg cttttctcgg cctgcgtgtt cgagccgttc
1861 gttcgacaga cgctcatggc actcgtgaat ggccatctcc tcgcagttat caatgacgtg
1921 gaaaaatatg atgccgatac gctcctgccg ttcatacgca gacacagcat cacctacctc
1981 aatggtactg cctctgtcct gcaagagtac gacttttccg actgcccatc actgaatcgg
2041 ataatcctgg tgggtgagaa cctgacagaa gcccggtatc tggcgctgcg ccagcggttc
2101 aagaatcgca tcctcaacga gtatggtttt accgagtcag cctttgtaac ggccctcaag
2161 attttcgacc cggagtcgac ccgtaaggac acgagtctgg ggagaccggt gcgcaacgtc
2221 aagtgctaca tcctcaatcc atcccttaaa cgtgtcccga ttggagctac gggtgagttg
2281 catatcggag ggttgggcat ttccaaggga tacctcaacc gccccgaact cacgccgcac
2341 cgcttcattc ccaacccctt ccaaacggat tgcgagaagc agctcgggat caacagcttg
2401 atgtacaaga ccggtgacct ggcccgctgg cttccgaacg gcgaggttga gtatctcgga
2461 cgcgcagatt tccagatcaa actgcgaggt attcgaattg aacctggtga aattgagacg
2521 atgctggcta tgtaccctag ggtccggacc agtttagtgg tgtccaaaaa gctccgcaac
2581 ggtccagagg aaactaccaa cgagcacctc gtgggttatt atgtttgtga tagcgcctca
2641 gtgtccgagg cagacctgct gtcattttta gagaagaaac tgcctcgata catgattccc
2701 acgcggttgg tacagctgtc gcagatccca gtgaatgtga acgggaaggc ggacctacgc
2761 gccttgccgg ccgtcgatat ctccaattcc acggaggtcc gttccgacct tcgaggcgat
2821 acggaaatcg ccctcgggga aatctgggcc gacgtgttgg gagcccgcca gagatccgtc
2881 tctcgcaacg acaacttctt ccgcctagga gggcacagca tcacctgcat ccaactgatc
2941 gctcgcatcc gacaacgaca acgactctcg gtcagcatct ccgtcgaaga tgtttttgca
3001 acaaggacac ttgagcgcat ggcagacctt ctacagaaca agcagcagga gaaatgcgac
3061 aaaccccatg aggcgccgac agagctgctt gaggagaatg cagcaacgga caatatctat
3121 ctggcaaaca gtcttcagca gggcttcgtc taccattacc tcaagagcat ggaacaatcc
3181 gacgcctatg taatgcagtc cgttcttcgg tacaacacca cattgtctcc agatctgttt
3241 cagagagcct ggaagcatgc acagcagtcc tttccagcgc tgcggctgcg gttctcatgg
3301 gaaaaggagg ttttccaact gctcgatcag gatccaccat tggactggcg tttcctctac
3361 ttcaccgacg ttgccgcggg tgctgtcgag gaccggaaat tggaagactt gcggcgccaa
3421 gaccttacgg agagattcaa gctggatgtt ggcagactgt tccgcgtcta tctgattaaa
3481 cacagcgaga atcgcttcac gtgtcttttc agctgccacc atgcaatcct cgatggttgg
3541 agtctgccac tcttgttcga aaaggttcac gagacctacc tgcaactgct gcatggggac
3601 aatctcactt cgtccatgga tgacccttac actcgcaccc agcggtatct ccacgctcac
3661 cgtgaggatc acctcgactt ttgggccggt gtggttcaaa agatcaacga acggtgtgat
3721 atgaacgcct tgttgaacga gcgcagtcgt tacaaagtcc agctggcaga ctatgaccag
3781 gtgcaggagc agcgacagct gacaattgct ctctctggag acgcatggct agcagacctt
3841 cgtcagacct gctccgccca gggtattacc ttacattcga ttctccaatt tgtttggcac
3901 gccgtgctgc acgcttatgg cggtggcacc cacaccataa ccggcacgac catttctgga
3961 aggaacctgc ccatcttggg aattgaacga gcagttggtc cgtatatcaa cactctaccg
4021 ctggtactcg atcattcgac gttcaaggat aagacaatca tggaggccat cgaggatgtg
4081 caggccaagg taaacgtcat gaacagccgg ggcaatgtgg aactgggccg tttgcacaaa
4141 accgacttaa agcacggatt attcgattct ttattcgtgc ttgaaaacta cccgaatttg
4201 gacaaatcgc gaacacttga gcaccagact gaactggggt attcgattga aggcggcact
4261 gagaagctga attatccact ggctgtcatc gcgcgcgaag tcgagacgac tggcggattc
4321 acagtatcca tctgctacgc cagtgagcta tttgaggagg ttatgatctc cgagcttctt
4381 catatggtcc aggacacact gatgcaggtt gcccgaggtt tgaatgaacc cgtcggcagc
4441 ctggagtatc tctcatctat ccaattggag caactcgccg cgtggaatgc cacggaagct
4501 gagtttcccg ataccacgct tcatgagatg tttgaaaacg aagcgagcca gaagccggac
4561 aagatagcag tggtctatga ggagacgtcc ttgacttacc gcgagttgaa tgagcgggcg
4621 aaccgtatgg cacatcagct aaggtccgac gtcagcccca accccaacga ggtcattgcg
4681 ctggtgatgg acaagagcga gcatatgatc gtcaacattc tggccgtatg gaagagcggc
4741 ggtgcctatg tccccattga ccctggatat cctaacgacc gcattcaata catcctagag
4801 gacacacaag ccctcgcagt catcgcggac tcctgctatc tgcctcgcat caagggaatg
4861 gctgcctccg gcacgcttct ttatccctct gtcttgcctg ccaatccgga ttccaagtgg
4921 agcgtatcga acccttcacc gttgagtcgg agcacggact tagcttatat catctatacc
4981 tctggaacga caggtcggcc caagggcgtc acggtagagc atcatggagt ggtcaacctg
5041 caggtgtcgc tatccaaagt attcggacta cgggatactg acgacgaggt aattctctcc
5101 ttttccaact atgtgttcga ccatttcgtg gagcagatga ccgacgccat tctcaatggc
5161 caaaccctcc tggtcctcaa cgatggaatg cgcggggaca aagagcgact ctacagatac
5221 attgagaaga accgagtgac ctacttgtct ggcaccccat ccgtggtctc catgtacgaa
5281 tttagccggt tcaaggacca tctacgccgt gtggactgcg tgggggaggc gttcagcgaa
5341 ccggtcttcg acaagatccg cgaaacgttc catggcctcg ttatcaacgg ctacggccca
5401 actgaagttt ccatcaccac ccacaagcgg ctctatccat tcccagagcg gcgaatggac
5461 aaaagtattg gccaacaggt ccacaatagc acgagctatg tgctgaacga ggacatgaag
5521 cgcaccccca taggggctgt cggcgagctc tacctgggtg gtgaaggagt ggtacgggga
5581 tatcacaatc gcgcagatgt gaccgcggag cgttttattc ctaatccatt ccagtcggaa
5641 gaagataagc gagaaggtcg taactcccgt ttgtacaaga ccggtgacct ggtacgctgg
5701 attcctggaa gcagcgggga ggtcgagtat ctaggtcgta atgacttcca ggtcaagatt
5761 cgcggactgc gcatcgaact aggcgagatt gaggccatcc tatcgtctta tcacggaatc
5821 aaacagtctg tggtgattgc caaggattgc agagaagggg cccagaaatt cctggttggt
5881 tactatgtcg ccgatgcagc gctgccgtcc gctgccattc ggcgcttcat gcagtctcgg
5941 ctccctggct acatggtgcc ctctcgtctc attctcgtca gcaagttccc cgtcactcct
6001 agtggaaaat tagacaccaa ggctttgccc ccagccgagg aagagagcga gattgacgtg
6061 gtgccgccgc gtagtgaaat cgaacgctcc ttgtgtgaca tctgggcgga actactcgag
6121 atgcacccag aggagatcgg catttacagc gatttcttca gcctgggagg tgacagccta
6181 aagagcacaa agctttcctt catgattcac gagtccttta accgcgccgt ctcagtcagc
6241 gcccttttct gtcaccggac agttgaagcc cagacgcact tgatcctgaa cgatgctgca
6301 gatgtgcacg aaattactcc catagattgc aatgatacgc agatgattcc cgtgtcccgt
6361 gcccaggagc gactcctctt catccacgaa tttgagaatg gcagcaatgc atacaatatc
6421 gacgctgcat ttgaactgcc tggctcggtt gacgcgtcgc ttctcgagca ggcgctgcgt
6481 ggaaaccttg ctcgacatga ggcgttgaga actttactgg tcaaggatca cgcaaccggc
6541 atctatcttc agaaggtatt gagtcccgat gaagcccagg gcatgttctc cgtcaacgtg
6601 gacacagcca agcaggtgga gcggctggac caggagatag ccagtctatc ccagcatgtt
6661 ttccgcctcg atgatgaact gccttgggag gcccgcatcc ttaaactcga atccggcggc
6721 ctgtatctca ttctggcgtt ccaccatacc tgcttcgatg catggtcatt gaaagtcttc
6781 gagcaagagc ttcgggcctt gtacgcagcg ctccagaaaa ccaaaagtgc agcgaactta
6841 ccagccctca aagcgcagta caaggaatac gcgctctacc atcgccggca gctgtctggc
6901 gatcgcatgc gcaacctgtc agacttttgg ctgcggaaac tcattggctt ggaaccattg
6961 cagctgatca cggaccgccc acgtcctgtg caattcaaat acgacggtga cgacctcagt
7021 atcgaactga gcaagaagga aacggagaac ctgagggggg tggccaaacg ttgcaagtcg
7081 agtctgtacg tcgtgttggt ttccgtttat tgcgttatgc tagcctcgta cgcgaaccag
7141 tccgatgttt ccgtgggtat cccagtcagc caccgaacgc atcctcagtt ccaatcggtc
7201 attggattct tcgtcaacct tgtggtgcta agggtggata tttctcagtc agccatttgc
7261 gggctcatca gaagggtaat gaaagagctc gtggacgccc aactgcacca agacatgccg
7321 ttccaggaag tgacgaagct gctgcaggtg gataatgacc ccagccggca tccgctggta
7381 cagaacgtgt tcaacttcga atcccgtgcg aacggagaac acgatgccag gtcggaggat
7441 gaaggatcgc ttgcattcaa tcaataccgg ccggttcagc ccgtggattc cgttgcgaag
7501 ttcgatctga acgcaacggt cacggaattg gagtcgggat tgagagtcaa cttcaactat
7561 gcgaccagcc tattcaacaa aagcacgatc cagggttttt tgcataccta tgagtatctc
7621 ctgcgccagc tgtccgaact gagtgcagaa gggatcaatg aggatacgca gctgtcgtta
7681 gttcgcccga cagagaatgg cgatctgcac ttgccattgg cacagtcccc gcttgcgacg
7741 actgctgagg agcagaaagt agcgtcgttg aaccaggcct ttgagcgcga agctttcctt
7801 gccgcagaga agattgccgt cgtgcaggga gatagagcac ttagttatgc tgatcttaac
7861 gggcaggcta accagctcgc ccggtacata cagtccgtgt cctgtattgg ggcagacgac
7921 ggaatagctt tgatgctgga aaagagtatc gacacgatta tttgcattct cgcgatttgg
7981 aaggctggtg cagcatacgt gcccttggat ccgacttacc cacccggacg cgtccagctg
8041 attctggagg agattaaagc gaaggctgtc cttgtgcact ccagtcatgc ttcgaaatgt
8101 gaacgccatg gcgcgaaggt gattgcagtc gactcgcccg ccatcgagac ggcggtcagc
8161 caacagtcag ctgctgacct gcccacaatt gctagcctcg gcaatctagc gtatataatc
8221 tttacttcag gcacttccgg taagccaaag ggagtcctag ttgagcaaaa ggcagttctt
8281 cttctacgcg atgccctccg ggagcggtat ttcggtcgag actgtaccaa gcatcatggc
8341 gtcctgttcc tgtccaacta cgtcttcgac ttctccgtcg aacaacttgt gttgtcggtg
8401 ctcagcggac acaagctgat cgttccccca gctgagttcg tcgcagatga tgaattttac
8461 agaatggcca gcacgcacgg tctctcctat ctcagcggca caccatcctt actgcagaag
8521 atcgatctgg cacgactgga ccatctgcag gttgttaccg ccgcgggcga agagcttcac
8581 gccacccagt acgagaagat gcgccgccga ttcaacggtc ccatctacaa tgcctatggt
8641 gtcaccgaga ccacggtgta caacattatc gcggaattca caacgaattc gatatttgag
8701 aatgctcttc gggaagtgct ccctggtacc cgagcgtatg tgctgaacgc ggcacttcag
8761 cccgtcccct tcgatgctgt cggagaactc tatcttgccg gcgacagcgt tacgcgtggt
8821 tatctcaacc aacctcttct aacggatcag cgattcattc ccaacccttt ctgcaaagag
8881 gaggacatcg ctatggggcg cttcgcgcgg ctctacaaga ccggcgacct ggttcgatcg
8941 cgtttcaacc gtcagcagca gccgcagctg gaatacctag gaagaggcga tctgcagatc
9001 aagatgaggg gataccggat cgagatttct gaagttcaga acgtgctcac ttcaagtccc
9061 ggtgtccggg agggtgcagt cgttgccaag tatgagaaca acgataccta ttcccggacc
9121 gctcactctc tggtcggtta ctataccacg gacaatgaaa cagtatcgga agccgatatt
9181 ctcactttca tgaaagcaag gcttccaacg tacatggtgc caagccacct ctgctgtctg
9241 gaaggcgcac tgcctgtgac gattaacgga aagctcgacg tccggagatt gccggagatt
9301 atcaacgact ccgcgcagtc ctcgtacagc ccaccaagga acataatcga ggccaagatg
9361 tgcagactgt gggaatccgc cttgggaatg gagcgatgcg gtatcgacga cgacctgttc
9421 aaactgggtg gcgacagcat cacatctttg catctcgtgg cccagattca caaccaggtg
9481 ggctgcaaga tcaccgttcg ggatatattt gaacatcgta ccgcccgagc cctccatgat
9541 cacgtcttca tgaaggactc cgaccggagt aatgtgactc agttccgaac cgaacaaggg
9601 ccggtcatcg gcgaggcgcc cctactgccg attcaagact ggtttttgtc aaaggctctg
9661 cagcatccga tgtattggaa tcacactttc tacgtccgaa cgccagagct ggatgttgat
9721 tccttaagcg ctgctgtcag ggacttgcaa cagtatcacg atgttttccg catgcgactc
9781 aagcgcgagg aagtcggatt cgtgcagtcc tttgctgagg acttctctcc tgcccagctt
9841 cgggtgctga acgtaaaaga tgttgacggg tccgcggccg tcaacgagat attggatggg
9901 tggcagtctg gcttcaacct tgagaacgga cccattggtt ccattggcta cctacatggg
9961 tatgaagacc gatccgcgcg agtctggttc tccgttcacc atatggccat tgacaccgtc
10021 agctggcaga tccttgtccg tgacctgcag acgctgtacc gaaatggaag cctcggaagc
10081 aagggcagca gtttccggca gtgggctgaa gccatccaaa attacaaggc gtcagactct
10141 gagaggaacc attggaataa gctcgtcatg gaaacagctt ccagcatatc cgcattgcct
10201 acgtcaaccg gttcgcgcgt gcgcctgagc agaagtttga gccctgagaa gacagcctca
10261 ctgatccaag gaggaatcga tcgacaggat gtctccgtgt acgactccct cctgacttca
10321 gttggattgg cgctccaaca tatcgctcca accggcccaa gtatggttac gatcgaggga
10381 catggccgtg aagaagtgga tcagacactg gatgtgagcc gcaccatggg ttggttcacc
10441 accatgtatc catttgaaat tccccgtctc agcaccgaga acattgttca aggagtcgtc
10501 gctgtgagcg aacggttcag acaggtgcct gcccgtggcg tcgggtatgg aaccttgtac
10561 ggctatactc aacacccgct gccccaggtg accgtcaact acctgggcca gctcgcccgc
10621 aagcaatcga agccaaagga atgggtcctc gcggtgggcg acaacgaatt tgaatacgga
10681 ctcatgacta gcccagagga caaagaccgg agctcttctg ccgtcgacgt cacggccgtg
10741 tgtattgacg gcactatgat catcgatgtg gacagtgctt ggagccttga ggagagcgag
10801 caattcatct cgagcatcga ggaaggactg aacaagatcc tcgacggcag ggcaagtcag
10861 caaacctcgc gattcccgga tgttcctcaa ccggcggaga catatacgcc gtatttcgag
10921 tatctggaac ctccacgaca gggaccgacg ctgttcctgc tgccgccggg cgaaggaggc
10981 gccgagagtt acttcaacaa catcgtcaag cgcctgcgtc agacaaatat ggtggtcttc
11041 aacaactact acttgcacag caaacgcctg cgcacgttcg aggagctggc ggaaatgtat
11101 ctcgaccaag tacgcggcat ccaaccacac ggaccgtacc acttcatcgg atggagcttc
11161 ggaggaattc tcgcaatgga aatgtcgcgg cgactggtag cctcggacga gaagattggc
11221 ttcctcggta ttatcgacac ctatttcaac gtgcggggag cgacacgcac cattggcttg
11281 ggggacactg agattctgga cccgatccat cacatctaca atcccgatcc ggccaacttc
11341 caacgcctgc cctctgcaac agatcgcatt gtgctgttca aggccatgag gccgaacaac
11401 aagtacgaat ccgagaacca gcgtcgcctg tacgagtact atgacgcact cgactcaacg
11461 gactggacag cttgttacca agcgattccg acgtccagct ggtcccgctt acggacgata
11521 cacacttttc ctgggtcgga aatccacaac aggtggagca gatgtgtgcg actatcaagg
11581 aacacctcgc tcgctattga tccgtcacta gcagcacagt atatcggacg atggaagtga
11641 tggagtgggg gggataggat acgatcaaac cagggtgcgg ttctttttgg ggggaactag
11701 tctctggttg aggaaagcga ggtagcaaat aaactaccaa ggtctagacc cacatagtct
11761 gtcattgttt tcgatcctaa attgatatat aataggcgac acctttagtt agccaaattt
11821 tctatataga aacaccacgg tttttaggag ttaggaccac gtcagaccgt ggccctttca
11881 ctaacgcttc cgtttacatc cataccggat gttgttgtag cacatttata tgatatcatt
11941 taagactata tacgcctatt cccccctatc gaataggccc ctacgtattt cttttgtttt
12001 tttctttttc tttttttttt ttttcttttc gctctctccc cttttatacc caatatcgga
12061 tcgagttgat aatatcaata tctaaaactc ccaattaaac ctacaaagcc tatcttagtg
12121 taagtgaatt tgggctctgg accaaattct ccgccaagga taatctttcc gataaacggt
12181 ggttatccgg tcatcataaa aaaggaaaag gtactccgtc ctcgataata aaacgtaaca
12241 taagcatgtc gttcaccata gacaagagga accaacatca ttaagcaggg gataggttca
12301 tccggtctag ggcgtcgagt gccaccgccc gtaggttgtc aatcttgagc tggatttgga
12361 gatc
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