GenBank-Updates@genbank.bio.net (11/16/90)
LOCUS ECOEPECAE 5220 bp ds-DNA BCT 16-NOV-1990
DEFINITION Escherichia coli attaching and effacing (eae) gene, complete cds.
ACCESSION M58154 M34051
KEYWORDS attaching and effacing histopathology; membrane protein.
SOURCE E.coli (subspecies EPEC, strain E2348/69) DNA, clone pCVD436.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 5220)
AUTHORS Jerse,A.E., Yu,J., Tall,B.D. and Kaper,J.B.
TITLE A genetic locus of enterophathogenic Escherichia coli necessary for
the production of attaching and effacing lesions on tissue culture
cells
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 7839-7843 (1990)
STANDARD simple staff_review
COMMENT The subspecies abbreviation EPEC stands for enteropathogenic
E.coli, as distinguished from EIEC, EHEC, or ETEC.
FEATURES from to/span description
pept 1114 1584 ORF
signal 1591 1596 -35 signal
signal 1614 1619 -10 signal
binding 1633 1636 ribosome binding site
pept 1644 4463 eae product
BASE COUNT 1525 a 990 c 1212 g 1493 t
ORIGIN
1 tccagcgttc gctcagatcc taaattctgg gtttctgtcg gcgcaattgc tgctggttta
61 gcgggactgg cggcaactgg tattgcacag ggcgttggct ttgacaccgg aaccggatga
121 tcctacaacc accgatcctg atcaggccgc aaatgctgca gaaagtgcaa caaaagatca
181 gttaacgcaa gaagcattca agaaccctga gaaccagaaa gttaacatcg atgcgaacgg
241 aaatgctatt ccgtctgggg aattaaaaga tgatattgtt gagcaaatag cacaacaagc
301 taaagaggct ggtgaggtgg ccagacagca ggctgttgaa agcaatgcac aggcgcagca
361 gcgatatgag gatcagcatg ccagacgtca ggaggaatta cagctttcat cgggtattgg
421 ttacggcctc agcagtgcat tgattgttgc tgggggaatt ggtgctggtg taacgactgc
481 gctccataga cgaaatcagc cggcagaaca gacaactact acaacaacac atacggtagt
541 gcagcaacag accggaggga taccccagca caaggtggca ctgatgccac aagagcagaa
601 gacgcttctc tgatagacgt gattcgcagg ggagtgttgc atcgacacac tggtcagatt
661 cctctagcga agtggttaat ccatatgctg aagttggggg ggctcggaat agtctatcgg
721 ctcatcagcc agaagagcat atttatgatg aggtcgctgc agatcctggt tatagcgtta
781 ttcagaattt ttcagggagc ggcccagtta ccggaaggtt aataggaact ccagggcaag
841 gtatccaaag tacttatgcg cttctggcaa acagcggcgg attgcgttta ggtatgggag
901 gattaacgag tggtggcgag acggcagtaa gttctgtaaa tgccgcacca acgccgggac
961 cagtacgttt cgtttaaata tatctgtgag tatttagttg aggttggggt ggggtggggg
1021 ggcgttttac tagcgttaat gtttcagaga acaacgttgc agcatgggta actcttgaac
1081 ttctgttatt ataatcaatt aagagaaatt ataatgtcat caagatctga acttttatta
1141 gataggtttg cggaaaaaat tggtgttgga tctatttcct ttaatgaaaa tagattgtgt
1201 tcttttgcta ttgatgaaat ttattatatt tcgttatctg atgccaatga cgaatatatg
1261 atgatttatg gcgtctgtgg aaaattcccg acagataacc ctaacttcgc tcttgagatt
1321 ttgaatgcaa acttatggtt tgcagagaat ggtgggccat acctgtgtta tgagtcagga
1381 gcacaatcgc tgttgttagc gttacgtttc ccgctcgatg atgctacccc tgaaaaactt
1441 gagaatgaaa tagaagtcgt cgttaagtcg atggaaaatc tgtatttggt attacataat
1501 cagggaataa cattagaaaa cgaacatatg aaaatagagg aaattagctc aagcgataat
1561 aaacattatt acgccggaag ataaaactta tttattagta ttaatttact actcattcta
1621 actcattgtg gtggagccca aacatgatta ctcatggttt ttatgcccgg acccggcaca
1681 agcataagct aaaaaaaaca tttattatgc ttagtgctgg tttaggattg tttttttatg
1741 ttaatcagaa ttcatttgca aatggtgaaa attattttaa attgggttcg gattcaaaac
1801 tgttaactca taatagctat cagaatcgcc ttttttatac gttgaaaaca ggtgaaactg
1861 ttgccgatct ttctaaatcg caagatatta atttatcgac gatttggtcg ttgaataagc
1921 atttatacag ttctgaaagc gaaatgatga aggccgagcc tggtcagcag atcattttgc
1981 cactcaaaaa acttcccttt gaatacagtg ccttaccact tttaggttcg gcacctcttg
2041 ttgctgcagg tggtgtcgct ggtcatacaa ataaactgac taaaatgtcc ccggacgtga
2101 ccaaaagcaa catgaccgat gacaaggcat taaattatgc ggcacaacag gcggcgagtc
2161 tcggtagcca gcttcagtcg cgatctctga acggcgatta cgcgaaagat accgctcttg
2221 gtatcgctgg taaccaggct tcgtcacagt tgcaggcctg gttacaacat tatggaacgg
2281 cagaggttaa tctgcagagt ggtaataact ttgacggtag ttcactggac ttcttattac
2341 cgttctatga ttccgaaaaa atgctggcat ttggtcaggt cggagcgcgt tacattgact
2401 cccgctttac ggcaaattta ggtgcgggtc agcgtttttt ccttcctgaa aatatgttgg
2461 gctataacgt cttcattgat caggattttt ctggtgataa tacccgttta ggtattggtg
2521 gcgaatactg gcgagactat ttcaaaagta gtgttaacgg ctatttccgc atgagcggct
2581 ggcatgagtc atacaataag aaagactatg atgagcgccc agcaaatggc ttcgatatcc
2641 gttttaatgg ctatctgcca tcatacccgg cattaggtgc caagctgatg tatgagcagt
2701 attatggtga taatgttgct ttgtttaatt ctgataagct gcagtcgaat cctggtgcgg
2761 cgaccgttgg tgtaaactat actccgattc ctctggtgac gatggggatc gattaccgtc
2821 atggtacggg taatgaaaat gatctccttt actcaatgca gttccgttat cagtttgata
2881 aaccgtggtc tcagcaaatt gagccacaat atgttaacga gttaagaaca ttatcaggca
2941 gccgttacga tctggttcag cgtaataaca atattattct ggagtacaaa aagcaggata
3001 ttctttctct gaatattccg catgatatta atggtactga acgcagtacg cagaagattc
3061 aattgatcgt taagagcaaa tacggtctgg atcgtatcgt ctgggatgat agtgcattac
3121 gtagccaggg cggccagatt cagcatagcg gaagccaaag cgcacaagat taccaggcta
3181 ttttgcctgc ttatgtgcaa ggtggtagca atgtttataa agtgacggct cgcgcctatg
3241 accgtaatgg caatagctct aacaatgtac tgcttactat taccgttctg tcgaatggtc
3301 aggtggtcga ccaggttggg gtaacggact ttacggctga taagacttcg gctaaagcgg
3361 atggcaccga agcaattact tatactgcga cggtgaaaaa gaatggggta gctcaggcta
3421 atgtccctgt ttcatttaat attgtttcag gaactgcagt tttaagtgcc aatagtgcca
3481 ataccaatgg tagcggtaag gcgactgtaa ccctgaaatc ggataaacca ggccaggtcg
3541 tcgtgtctgc taaaacagca gagatgactt cagcgcttaa tgccaatgca gttatatttg
3601 ttgatcaaac caaggccagc attactgaga ttaaggctga taaaacaacg gcagtagcaa
3661 atggtcagga tgctattaca tacactgtta aagtgatgaa gggggataag cctgtatcta
3721 atcaggaagt gacctttacg acgaccttag gtaagttaag taattccact gaaaaaacgg
3781 atacgaatgg ctatgccaaa gtaacattaa catcgacaac tccaggaaaa tcactcgtta
3841 gtgcccgtgt tagcgatgtc gccgttgatg tcaaagcacc tgaagttgaa ttttttacaa
3901 cgcttacaat tgatgacggt aatattgaaa ttgttggaac cggagttaaa gggaagttac
3961 ccactgtatg gttgcaatat ggtcaagtta atctgaaagc cagcggaggt aacggaaaat
4021 atacatggcg ctcagcaaat ccagcaattg cttcggtgga tgcttcttct ggtcaggtca
4081 ccttaaaaga gaagggaact acaactattt ccgttatctc aagtgataat caaactgcaa
4141 cttatactat tgcaacacct aatagtctga ttgttcctaa tatgagcaag cgtgtgacct
4201 ataatgatgc tgtgaataca tgtaagaatt ttggaggaaa gttgccgtct tctcagaatg
4261 aactggaaaa tgtctttaaa gcatgggggg ctgcaaataa atatgaatat tataagtcta
4321 gtcagactat aatttcatgg gtacaacaaa cagctcaaga tgcgaagagt ggtgttgcaa
4381 gtacatacga tttagttaaa caaaaccctc tgaataatat taaggctagt gaatctaatg
4441 cttatgccac ttgtgtaaaa taaatttaat aaatatctaa tcattgtccg gctaaaatgt
4501 atcttttagt cggacatttt tctgcgctat attctatatt agcacctttc gagttgtatc
4561 ctggtaatca agtctctagc tagcttttct ctccacccaa taatttgaca tcatgaagac
4621 tatatctata acatccacac aataaaaaac cctccgaaga gggggaagag gataagaaat
4681 taatacgaca gtggaatatg tatatattcg tctgggcggg agatgttgat accatcttta
4741 ggatcaattg cgtcgatgac atagcctcct ttcagaatgc taccaatccc gaggctggta
4801 ccattatcaa gagcaattgt aggattcttc atcgtaccgc tgattctgag tatttttccg
4861 ggaaaaatat ctgctgaaat gtacggctga atgttctgaa agagtatctt gactgttact
4921 atttttgttc attgcattta taatggcaag gatcttactc tcgttctgtt gagaaacttc
4981 ccccgcgaca atgatgacat cattcttttt gaaaatattg acatagttta ttagtttatt
5041 ctttttaaac tctgatgcca gttcattgat tttattgtta ctgttgttgt gtatttccca
5101 tcctgcaacc cctggcgtat tcagtaacag attttcaacc tcgctccatt tgggtgaagg
5161 gggaatgtat cccgataaca gaaagaaccc aggcttatta ccttttgtaa taatgatgat
//