GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS PTONOMPA 2846 bp ds-DNA SYN 22-DEC-1990 DEFINITION Cloning vector DNA, clone pTO-N. ACCESSION M59367 M34008 KEYWORDS . SOURCE Cloning vector DNA, clone pTO-N. ORGANISM cloning vector REFERENCE 1 (bases 1 to 2846) AUTHORS Deng,T., Noel,J.P. and Tsai,M.-D. TITLE A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli: Overproduction of bovine pancreatic phospholipase A-2 JOURNAL Gene 93, 229-234 (1990) STANDARD simple staff_entry FEATURES Location/Qualifiers promoter 21..39 /evidence=EXPERIMENTAL misc_feature 81..143 /note="OmpA signal" BASE COUNT 704 a 722 c 740 g 680 t ORIGIN 1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc 61 tagataacga ggcgcaaaaa atgaaaaaga cagctatcgc gattgcagtg gcactggctg 121 gtttcgctac cgtagcgcag gccatgggaa ttccaagctt ggatccggct gctaacaaag 181 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 241 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggataat 301 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 361 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 721 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc 781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 841 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 901 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 961 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 1021 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta 1081 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 1141 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 1201 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 1261 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 1321 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 1381 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 1441 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 1501 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 1561 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 1621 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 1681 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 1741 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 1801 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 1861 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 1921 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 1981 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 2041 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 2101 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 2161 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 2221 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 2281 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat 2341 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 2401 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac 2461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 2521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 2581 aacgcgcgag gcagattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg 2641 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg 2701 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 2761 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 2821 ccacgatgcg tccggcgtag aggatc //