GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS PTONOMPA 2846 bp ds-DNA SYN 22-DEC-1990
DEFINITION Cloning vector DNA, clone pTO-N.
ACCESSION M59367 M34008
KEYWORDS .
SOURCE Cloning vector DNA, clone pTO-N.
ORGANISM cloning vector
REFERENCE 1 (bases 1 to 2846)
AUTHORS Deng,T., Noel,J.P. and Tsai,M.-D.
TITLE A novel expression vector for high-level synthesis and secretion of
foreign proteins in Escherichia coli: Overproduction of bovine
pancreatic phospholipase A-2
JOURNAL Gene 93, 229-234 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
promoter 21..39
/evidence=EXPERIMENTAL
misc_feature 81..143
/note="OmpA signal"
BASE COUNT 704 a 722 c 740 g 680 t
ORIGIN
1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
61 tagataacga ggcgcaaaaa atgaaaaaga cagctatcgc gattgcagtg gcactggctg
121 gtttcgctac cgtagcgcag gccatgggaa ttccaagctt ggatccggct gctaacaaag
181 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
241 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggataat
301 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
361 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
721 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
841 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
901 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
961 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
1021 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
1081 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
1141 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
1201 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
1261 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
1321 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
1381 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
1441 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
1501 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
1561 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
1621 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
1681 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
1741 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
1801 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
1861 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
1921 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
1981 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
2041 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
2101 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
2161 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
2221 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
2281 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
2341 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
2401 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
2461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
2521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
2581 aacgcgcgag gcagattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
2641 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
2701 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
2761 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
2821 ccacgatgcg tccggcgtag aggatc
//