GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS ECOFADAB 4104 bp ds-DNA BCT 22-DEC-1990
DEFINITION E.coli fatty acid oxidizing complex (fadA, fadB) genes, complete
cds.
ACCESSION M59368 M36149
KEYWORDS fatty acid oxidizing complex.
SOURCE E.coli (strain K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4104)
AUTHORS DiRusso,C.C.
TITLE Primary sequence of the Escherichia coli fadBA operon, encoding the
fatty acid-oxidizing multienzyme complex, indicates a high degree
of homology to eucaryotic enzymes
JOURNAL J. Bacteriol. 172, 6459-6468 (1990)
STANDARD simple staff_review
COMMENT The fatty acid-oxidizing complex encoded within the fadB and fadA
genes exhibit the enzymatic activites of these enzymes: enol-CoA
hydratase (E.C. 4.2.1.17), 3-hydroxyacyl-CoA dehydrogenase (E.C.
1.1.1.35), vinylacetyl-CoA delta-isomerase (E.C. 5.3.3.3), and
acetyl-CoA acyltransferase (E.C. 2.3.1.16).
FEATURES Location/Qualifiers
CDS 326..2515
/gene="fadB"
/codon_start=326
/product="fatty acid oxidizing complex"
CDS 2525..3688
/gene="fadA"
/codon_start=2525
/product="fatty acid oxidizing complex"
BASE COUNT 950 a 1085 c 1172 g 897 t
ORIGIN
1 gatcgtcgag aaaaacattg aacagctcgc cggagtgaat aagtaacgca tccagcttga
61 agccggccag cgcatcgcga gtccgttctt gtaaggtagc tatatgattt ttatagagcg
121 aggccagtga ttccattttt tacccttctg tttttttgac cttaagtctc cgcatcttag
181 cacatcgttc atccagagcg tgatttctgc cgagcgtgat cagatcggca tttctttaat
241 cttttgtttg catattttta acacaaaata cacacttcga ctcatctggt acgaccagat
301 caccttgcgg attcaggaga ctgacatgct ttacaaaggc gacaccctgt accttgactg
361 gctggaagat ggcattgccg aactggtatt tgatgcccca ggttcagtta ataaactcga
421 cactgcgacc gtcgccagcc tcggcgaggc catcggcgtg ctggaacagc aatcagatct
481 aaaagggctg ctgctgcgtt cgaacaaagc agcctttatc gtcggtgctg atatcaccga
541 atttttgtcc ctgttcctcg ttcctgaaga acagttaagt cagtggctgc actttgccaa
601 tagcgtgttt aatcgcctgg aagatctgcc ggtgccgacc attgctgccg tcaatggcta
661 tgcgctgggc ggtggctgcg aatgcgtgct ggcgaccgat tatcgtctgg cgacgccgga
721 tctgcgcatc ggtctgccgg aaaccaaact gggcatcatg cctggctttg gcggttctgt
781 acgtatgcca cgtatgctgg gcgctgacag tgcgctggaa atcattgccg ccggtaaaga
841 tgtcggcgcg gatcaggcgc tgaaaatcgg tctggtggat ggcgtagtca aagcagaaaa
901 actggttgaa ggcgcaaagg cggttttacg ccaggccatt aacggcgacc tcgactggaa
961 agcaaaacgt cagccgaagc tggaaccact aaaactgagc aagattgaag ccaccatgag
1021 cttcaccatc gctaaaggga tggtcgcaca aacagcgggg aaacattatc cggcccccat
1081 caccgcagta aaaaccattg aagctgcggc ccgttttggt cgtgaagaag ccttaaacct
1141 ggaaaacaaa agttttgtcc cgctggcgca taccaacgaa gcccgcgcac tggtcggcat
1201 tttccttaac gatcaatatg taaaaggcaa agcgaagaaa ctcaccaaag acgttgaaac
1261 cccgaaacag gccgcggtgc tgggtgcagg cattatgggc ggcggcatcg cttaccagtc
1321 tgcgtggaaa ggcgtgccgg ttgtcatgaa agatatcaac gacaagtcgt taaccctcgg
1381 catgaccgaa gccgcgaaac tgctgaacaa gcagcttgag cgcggcaaga tcgatggtct
1441 gaaactggct ggcgtgatct ccacaatcca cccaacgctc gactacgccg gatttgaccg
1501 cgtggatatt gtggtagaag cggttgttga aaacccgaaa gtgaaaaaag ccgtactggc
1561 agaaaccgaa caaaaagtac gccaggatac cgtgctggcg tctaacactt caaccattcc
1621 tatcagcgaa ctggccaacg cgctggaacg cccggaaaac ttctgcggga tgcacttctt
1681 taacccggtc caccgaatgc cgttggtaga aattattcgc ggcgagaaaa gctccgacga
1741 aaccatcgcg aaagttgtcg cctgggcgag caagatgggc aagacgccga ttgtggttaa
1801 cgactgcccc ggcttctttg ttaaccgcgt gctgttcccg tatttcgccg gtttcagcca
1861 gctgctgcgc gacggccggg atttccgcaa gatcgacaaa gtgatggaaa aacagtttgg
1921 ctggccgatg ggcccggcat atctgctgga cgttgtgggc attgataccg cgcatcacgc
1981 tcaggctgtc atggcagcag gcttcccgca gcggatgcag aaagattacc gcgatgccat
2041 cgacgcgctg tttgatgcca accgctttgg tcagaagaac ggcctcggtt tctggcgtta
2101 taaagaagac agcaaaggta agccgaagaa agaagaagac gccgccgttg aagacctgct
2161 ggcagaagtg agccagccga agcgcgattt cagcgaagaa gagattatcg cccgcatgat
2221 gatcccgatg gtcaacgaag tggtgcgctg tctggaggaa ggcattatcg ccactccggc
2281 ggaagcggat atggcgctgg tctacggcct gggcttccct ccgttccacg gcggcgcgtt
2341 ccgctggctg gacaccctcg gtagcgcaaa atacctcgat atggcacagc aatatcagca
2401 cctcggcccg ctgtatgaag tgccggaagg tctgcgtaat aaagcgcgtc ataacgaacc
2461 gtactatcct ccggttgagc cagcccgtcc ggttggcgac ctgaaaacgg cttaaggagt
2521 cacaatggaa caggttgtca ttgtcgatgc aattcgcacc ccgatgggcc gttcgaaggg
2581 cggtgctttt cgtaacgtgc gtgcagaaga tctctccgct catttaatgc gtagcctgct
2641 ggcgcgtaac ccggcgctgg aagcggcggc cctcgacgat atttactggg gttgtgtgca
2701 gcagacgctg gagcagggtt ttaatatcgc ccgtaacgcg gcgctgctgg cagaagtacc
2761 acactctgtc ccggcggtta ccgttaatcg cttgtgtggt tcatccatgc aggcactgca
2821 tgacgcagca cgaatgatca tgactggcga tgcgcaggca tgtctggttg gcggcgtgga
2881 gcatatgggc catgtgccga tgagtcacgg cgtcgatttt caccccggcc tgagccgcaa
2941 tgtcgccaaa gcggcgggca tgatgggctt aacggcagaa atgctggcgc gtatgcacgg
3001 tatcagccgt gaaatgcagg atgcctttgc cgcgcggtca cacgcccgcg cctgggccgc
3061 cacgcagtcg gccgcattta aaaatgaaat catcccgacc ggtggtcacg atgccgacgg
3121 cgtcctgaag cagtttaatt acgacgaagt gattcgcccg gaaaccaccg tggaagccct
3181 cgccacgctg cgtccggcgt ttgatccagt aaacggtatg gtaacggcgg gcacatcttc
3241 tgcactttcc gatggcgcag ctgccatgct ggtgatgagt gaaagccgcg cccatgaatt
3301 aggtcttaag ccgcgcgctc gtgtgcgttc gatggcggtc gttggttgtg acccatcgat
3361 tatgggttac ggcccggttc cggcctcgaa actggcgctg aaaaaagcgg ggctttctgc
3421 cagcgatatc ggcgtgtttg aaatgaacga agcctttgcc gcgcagatcc tgccatgtat
3481 taaagatctg ggactaattg agcagattga cgagaagatc aacctcaacg gtggcgcgat
3541 cgcgctgggt catccgctgg gttgttccgg tgcgcgtatc agcaccacgc tgctgaatct
3601 gatggaacgc aaagacgttc agtttggtct ggcgacgatg tgtatcggtc tgggtcaggg
3661 tattgcgacg gtgtttgagc gggtttaacc acttgccgga tgcggcgtga acgccttatc
3721 cggtctaccg atccggcacc aatgtaggcc tgataagacg cgccagcgtc gcatcaggca
3781 tcatgcacca attgccggat gcggcgtaaa cgccttatcc ggcctacggt tcggactatt
3841 tgtaggcacg ataagacgcg acagcgtcgc atcaggcatc atgcaccaat tgccggatgc
3901 ggcgtaaacg ccttatccgg cctacggttc ggactatttg taggcacgat aagacgcgac
3961 agcgtcgcat cgggcatggt ttgccagata cgtcatagat ctaaatgcca ctgaataaca
4021 gtttagttgc cgttcttccc gcctgtcagg ggcgggtttt tttatatctc agataaatgc
4081 aaacgcatcg ccaaacaggc gatc
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