GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS TOBGLUCA 4766 bp ds-DNA PLN 22-DEC-1990
DEFINITION N.tabacum basic-1,3-glucanse gene, complete cds.
ACCESSION M59442 M34086
KEYWORDS glucan endo-1,3-a-D-glucan glucanohydrolase; glucanase.
SOURCE N.tabacum (var.Samsun NN) leaf DNA, clone GGLB50.
ORGANISM Nicotiana tabacum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (bases 1 to 4766)
AUTHORS Linthorst,H.J., Melchers,L.S., Mayer,A., Van Roekel,J.S.,
Cornelissen,B.J. and Bol,J.F.
TITLE Analysis of gene families encoding acidic and basic beta-1,
3-glucanases of tobacco
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 8756-8760 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS join(1589..1646,2434..3455)
/EC_number=3.2.1.39
/gene="glucanase"
/product="basic beta-1,3-glucanase"
mRNA join(1589..1646,2434..3676)
/gene="glucanase"
/partial
/product="basic beta-1,3-glucanase"
mRNA join(1589..1646,2434..3719)
/gene="glucanase"
/partial
/product="basic beta-1,3-glucanase"
intron 1647..2433
/gene="glucanase"
exon 2434..3676
/number=2
/gene="glucanase"
/codon_start=2434
exon 2434..3719
/gene="glucanase"
/codon_start=2434
BASE COUNT 1509 a 893 c 811 g 1553 t
ORIGIN
1 ctcgaggtcg ggctggatgc cacaataacc ctaaagtcga actccccgat gttggctgta
61 tacaattatg attaaaaaag ttccaccaag attgtagctc ctctaggggc caagggcaaa
121 cctttttgct aatgggaaaa aaatattaga ccaagagttt gtaatagcta ctcaatttca
181 atttacaaag gggaaaattt aactgttttg cccttatatc ttttggtccc gaaacataaa
241 atatcccatc cgaaattcca aatggtccat tatcggcaag tagctttctt ttattatagt
301 tagttgacaa aacactatcg agatatcatt agtataataa taacttcaaa gtccatctta
361 cttagctgcc tcctcattag agccgccact aaaataagac cgatcaaata aaagccgcca
421 ttaaaataat gaattttagg actctcaatt gtcacgtaag tcccaaaaac tcttccaata
481 ctttgctgca acttggggct gctagcttct gagcttctgg gatatttcta tgtttatctc
541 ttaattacat ctcaactaat atcaagaaat taaacaggtg cagcaaatca taaaattttc
601 ctctaaagaa gaaaatgact ccggttactg attcattggc ctttcagagt ctgcgtgcca
661 tattcactaa tgggtcgttt ggtacaagaa ataatgataa taatttcgaa atagaatttg
721 ggatttcatt tatttcatat ttaattataa atattagcta atttcgaaat aaattttaca
781 ttaaaatagt gaaatcaact atctcatata taaggtggaa tagctaatct catagccacc
841 tcagttagaa tccagtttcc tctaataaat gcagcgaaat attagaagac tttcattaaa
901 tcaattcata taatttaaaa tactagacat ggaaaaaaaa aacgattcga gactgttatg
961 gaaggcgttg ccttcgatgt agattctcat ccattgcttt cgtgcaatag caatatgaca
1021 tcttattctt agaactactt ttaaatgaaa gtcatataga actttaaaat ctctcaacta
1081 gttttaaggg aattcaaaat acgaccaata tttattactt acttatggat aaatttaaat
1141 aatatgtatt ttatcttgaa attgaattga aaatattaaa ttacttggtt taatataaac
1201 aatagatatc gttaagtatt taccacaaac attttattag ttgtaacgat gattaagcag
1261 gaattcctct ggttgtgcag gatgaaagaa actaactagc tataatttct tttgtaaagt
1321 caagatagta cggcacctta tggagaaaat aaataacttt acatcatcaa actcatcttc
1381 ttttttccac aaaatgatca agttgacatg ttaatagcca ggtcaccggg ggcggctctt
1441 aacttcatta gcctacgaat aacaaatcca atattatatt tacacaaggc tatatatata
1501 tctcaatata aatagctcgt tgttcatctt aattctccca acaagtcttc ccatcatgtc
1561 tacctcacat aaacataata ctcctcaaat ggctgctatc acactcctag gattactact
1621 tgttgccagc agcattgaca tagcaggttt ctggtcaaat atttgaactt cccagccaaa
1681 aatattgtct tataattttg tgtgcgcaaa attttaattt agttgatagt tatttgctta
1741 tttttctttt caaattgctt gtgttttttt ctcaaattaa cttgcaccgt attcatttag
1801 cgatagttat ttgctctatt ttgtgtaaca ctcactcaca aacttttcaa tttgagggga
1861 ggacagtgaa tctaagattg aaatttatga gtttaattag actaattccc atttgattta
1921 ttggctagaa gtcaattatt tgcatagtga gtcttttaac acacagattt gagttaaagc
1981 tactacgttc gtattaaccc ataacatata caccttctgt tctaatttct ttgacacttt
2041 ttgttagttt gttccaaaaa ggacggacat atttgatatt tgagaatact ttaccttaac
2101 cttaatagaa ttttttatga catcacatat attatggaat atatacgacc ataattttca
2161 aatatcttat agtcgtacaa atattatagc atgtttaata ccacaacttt caaattcttc
2221 ttttccttaa aaacaaaata tgtcacataa attaaaatag aggaagtata ctacatcaat
2281 cagcccctag tggaggggac ctactgtaag tttttaagtt ttcaagaatt cagtaattga
2341 ttaggagccc gtctggacat aaaaaaaaat tccttttttt ccaaaaaatg cccactaaat
2401 ttctaacact attttgtaat tcttattgag caggggctca atcgataggt gtttgctatg
2461 gaatgctagg caacaacttg ccaaatcatt gggaagttat acagctctac aagtcaagaa
2521 acataggaag actgaggctt tatgatccaa atcatggagc tttacaagca ttaaaaggct
2581 caaatattga agttatgtta ggacttccca attcagatgt gaagcacatt gcttccggaa
2641 tggaacatgc aagatggtgg gtacagaaaa atgttaaaga tttctggcca gatgttaaga
2701 ttaagtatat tgctgttggg aatgaaatca gccctgtcac tggcacatct tacctaacct
2761 catttcttac tcctgctatg gtaaatattt acaaagcaat tggtgaagct ggtttgggaa
2821 acaacatcaa ggtctcaact tctgtagaca tgaccttgat tggaaactct tatccaccat
2881 cacagggttc gtttaggaac gatgctaggt ggtttgttga tgccattgtt ggcttcttaa
2941 gggacacacg tgcaccttta ctcgttaaca tttaccccta tttcagttat tctggtaatc
3001 caggccagat ttctctcccc tattctcttt ttacagcacc aaatgtggtg gtacaagatg
3061 gttcccgcca atataggaac ttatttgatg caatgctgga ttctgtgtat gctgccctcg
3121 agcgatcagg aggggcatct gtagggattg ttgtgtccga gagtggctgg ccatctgctg
3181 gtgcatttgg agccacatat gacaatgcag caacttactt gaggaactta attcaacacg
3241 ctaaagaggg tagcccaaga aagcctggac ctattgagac ctatatattt gccatgtttg
3301 atgagaacaa caagaaccct gaactggaga aacattttgg attgttttcc cccaacaagc
3361 agcccaaata taatatcaac tttggggtct ctggtggagt ttgggacagt tcagttgaaa
3421 ctaatgctac tgcttctctc gtaagtgaga tgtgagctga tgagacactt gaaatctctt
3481 tacatacgta ttccttggat ggaaaaccta gtaaaaacaa gagaaatttt ttctttatgc
3541 aagatactaa ataacattgc atgtctctgt aagtcctcat ggattgttat ccagtgacga
3601 tgcaactctg agtggtttta aattcctttt ctttgtgata ttggtaattt ggcaagaaac
3661 tttctgtaag tttgtgaatt tcatgcatca ttaattatac atcagttcca tgtttgatca
3721 gattgggatt tggtaacttc aatgttagta ttataattag tgtctttatc attgactatc
3781 aattaatctt tatttggcaa ggcttgatat atttgagtta ctcttaggta tttgcaagca
3841 actgatcttt cttttatccc gtttctggct taaacctcat tagaaatata ttataatgtc
3901 acctactctg tggtttaaga cattccctta cattataagg tatttcacgt cgtatcaggt
3961 cgaaaaaaat aatggtacgc tctttcttat cacaaatttc tctcaacttc tagaccaatt
4021 gaatcttgtc tcccaataag cattgctttt actgtatgtt tctctctatc aattcaaggc
4081 tcaagccatc aaatcgcttg gtatttctcg ctcccattta accaatcgag ctgttagctc
4141 tgctaaagtc tcattcttca gcttctcaaa ccactcagag cttctccaac caaaaatgta
4201 gaaaaaatct ttgatccaca tgtaaaaccc cagaattgtc attcgagtga aggaaggtgc
4261 tggaaacgcg acatcaaaca cggatttgcc agctgattgt cataggaaaa agaaaagttg
4321 atcagatagt tcgtgcgggg tatttcctac ctcgcctttg atcatagctc tagggcttgc
4381 gaaactaagt tatgcacgag gcccccctgc gaatccataa atctaagaag catgcccaca
4441 acaaacggga taacttccag ctaagagatc tattttgatc ttacccacaa actagctccg
4501 tgtgaattgt ccatttcatc aactccaaag gcaggaaaga agactctatt cagagagcag
4561 caaaagagct ctacttgcct agtgagggtc taaagccata ttgttccccc gagtgatttt
4621 ggtcttgatg attgtcaaag gaacacatgt atgaaacaag tccatggata gtgtacatag
4681 ggcatggtca tattcaaaca aaaggcatgc acgtgcaagg gatacgtata agtggttata
4741 tctgataatc cctgaacgaa aagctt
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