GenBank-Updates@genbank.bio.net (12/19/90)
LOCUS PSELSP 2396 bp ds-DNA BCT 119-DEC-1990
DEFINITION P.fluorescens signal peptidase II (lsp) gene, complete cds and
isoleucyl-tRNA synthetase (ileS) gene, 3' end.
ACCESSION M35366
KEYWORDS isoleucyl-tRNA synthetase; signal peptidase II.
SOURCE P.fluorescens (strain NCIB 10586) DNA.
ORGANISM Pseudomonas fluorescens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 2396)
AUTHORS Isaki,L., Beers,R. and Wu,H.C.
TITLE Nucleotide sequence of the Pseudomonas fluorescens signal peptidase
II gene (lsp) and flanking genes
JOURNAL J. Bacteriol. 172, 6512-6517 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by H.Wu, 18-JUN-1990.
Author address:H.Wu
Uniformed Services University of the Health Sciences
4301 Jones Bridge Rd.
Bethesda, MD 20814
FEATURES Location/Qualifiers
CDS <1..729
/gene="ileS"
/codon_start=1
/product="isoleucyl-tRNA synthetase"
CDS 722..1234
/gene="lsp"
/product="signal peptidase II"
repeat_region 768..810
/note="inverted repeat"
CDS 1227..1679
/note="ORF149"
repeat_region 1239..1268
/note="inverted repeat"
repeat_region 1640..1674
/note="inverted repeat"
repeat_region 1697..1889
/note="inverted repeat"
CDS 1857..>2396
/note="ORF316"
BASE COUNT 498 a 706 c 712 g 478 t 2 others
ORIGIN
1 ctgcagcgcg agttgcagga acactacggc gaataccgct tctggaacgt ctactccaag
61 atccacaact tctgcgtgca ggagctgggt ggtttctacc tcgacatcat caaggaccgc
121 cagtacacca ctggcgccaa cagcaaggcg cgccgttcgg cagaccgcgc tgtaccacat
181 cagcgaaggc tggtgcgctg gatcgcaccg atcctggcat tcaccgctga cgaactgtgg
241 gagtacctgc cgggcgagcg taacgagtcc gtcatgctca acacctggta cgaaggcctg
301 accgaactgc cggctgactt cgaactgggc cgcgagtact gggagggcgt gatggccgtc
361 aaggttgcgg tgaacaagga actggaagtc cagcgtgcgg ccaaggccgt gggtggcaac
421 ctgcaagccg aagtcaccct gtttgccgaa gacggcctga ccgccgacct ggccaagctg
481 agcaacgagc tgcgcttcgt gctgatcacc tctactgcga gcctggcgcc gtttacccag
541 gctccggcag atgcggtggc taccgaagtg cctggcctta agctcaaagt ggtcaagtcg
601 gccttcccca agtgcgctcg ttgctggcac tgccgtgaag acgtcggcgt gaaccctgag
661 cacccggaaa tctgcggtcg ttgtgtcgac aacatcagcg gtgaaggcga ggttcgccac
721 tatgcctaat gcagacagtc gtttcggacg tctgggctgg ctcgtactga gcctgctggt
781 cctggtcatt gaccaggtca gcaaggctca cttcgagggc tccctggaaa tgttccagca
841 aatcgtggtg atcccggatt atttcagctg gaccctggcc tacaacactg gcgccgcctt
901 cagcttcctc gctgacggcg gtggctggca gcgctggctg tttgctgtga tcgccgtggt
961 ggtaagtgcc gtactggtgg tgtggctcaa gcgcctgggc cgcgacgaca cctggctggc
1021 catcgcgctg gccctagtgt tgggtggcgc gctgggcaac ctgtatgacc gcatcgccct
1081 gggccatgtg atcgacttta tcctggtgca ttggcagaac cgccactact tcccggcgtt
1141 caactttgcc gacagtgcta tcaccgtcgg cgcaatcatg ctggcgctgg atatgttcaa
1201 gagcaagaaa accggagaaa ccgtcaatga ctgatcaggt attggctgag caacgcatcg
1261 gccagaacac ggaagtcact ttgcatttcg cactgcgcct ggagaatggc gacacggtcg
1321 acagcacgtt cgacaaggcc cccgcgacct tcaaggtcgg cgacggcaac ctgctgccgg
1381 gtttcgaagc ggcactgttc ggtttcaagg ccggtgacaa gcgcaccctg caaatcctgc
1441 cggaaaacgc ctttggccag cccaacccgc aaaacgtgca gatcatcccc cgttcgcagt
1501 tccagaacat ggacctgtcg gaaggcttgc tggtgatctt caatgatgcg gcgaacactg
1561 aattgcctgg cgtggtgaaa gcatttgatg acgcgcaagt gaccatcgac ttcaatcacc
1621 cgttggccgg caagacgttg acctttgacg tcgagattat cgacgtcaaa gcgctgtaac
1681 cgaccgtacg cggtctaaaa tgtgggaggg ggcttgctcc cgatagcggt atatcagcta
1741 aatgtgcaga ctgatatacc tatatcggga gcaagccccc tcccaccttt gatctccatt
1801 gtcggttgag tcagtgtcaa attgactcaa tatggctgca atacacgagg cacagcatgc
1861 aaatcaaact cgccaacccc cgtggcttct gcgccggcgt ggaccgggcg atcgaaatcg
1921 tcaatcgcgc cctggaagtc ttcgggccgc cgatttatgt gcgccatgaa gtcgtccata
1981 acaaatttgt ggtcgaagac ttgcgtgcgc gcggggcgat ctttgtcgaa gaactcgatc
2041 aggtgaaaga cgacgtgatc gtcatcttca gtgcccacgg tgtttcccag gctgtacgta
2101 ccgaagcggc gggccgtggc ctgaaggtgt tcgatgccac ctgcccactg gtgaccaagg
2161 tgcatatcga ggtggcgcgc tacagccgcg acggtcgtga gtgcatcctg atcggccacg
2221 ccggtcaccc ggangtagaa ggcaccatgg gccaatacga cgccagcaac ggcggtgcta
2281 tctacctggt cgaggacgag aaagacgtcg ccaacttgca ggtgcacaat ccggaacgcc
2341 tggccttcgt gacccagacc accttgtcca tggacgacac cagccgngta atcgat
//GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS PSELSP 2396 bp ds-DNA BCT 22-DEC-1990
DEFINITION P.fluorescens signal peptidase II (lsp) gene, complete cds and
isoleucyl-tRNA synthetase (ileS) gene, 3' end.
ACCESSION M35366
KEYWORDS isoleucyl-tRNA synthetase; signal peptidase II.
SOURCE P.fluorescens (strain NCIB 10586) DNA.
ORGANISM Pseudomonas fluorescens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 2396)
AUTHORS Isaki,L., Beers,R. and Wu,H.C.
TITLE Nucleotide sequence of the Pseudomonas fluorescens signal peptidase
II gene (lsp) and flanking genes
JOURNAL J. Bacteriol. 172, 6512-6517 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by H.Wu, 18-JUN-1990.
Author address:H.Wu
Uniformed Services University of the Health Sciences
4301 Jones Bridge Rd.
Bethesda, MD 20814
FEATURES Location/Qualifiers
CDS <1..729
/gene="ileS"
/codon_start=1
/product="isoleucyl-tRNA synthetase"
CDS 722..1234
/gene="lsp"
/product="signal peptidase II"
repeat_region 768..810
/note="inverted repeat"
CDS 1227..1679
/note="ORF149"
repeat_region 1239..1268
/note="inverted repeat"
repeat_region 1640..1674
/note="inverted repeat"
repeat_region 1697..1889
/note="inverted repeat"
CDS 1857..>2396
/note="ORF316"
BASE COUNT 498 a 706 c 712 g 478 t 2 others
ORIGIN
1 ctgcagcgcg agttgcagga acactacggc gaataccgct tctggaacgt ctactccaag
61 atccacaact tctgcgtgca ggagctgggt ggtttctacc tcgacatcat caaggaccgc
121 cagtacacca ctggcgccaa cagcaaggcg cgccgttcgg cagaccgcgc tgtaccacat
181 cagcgaaggc tggtgcgctg gatcgcaccg atcctggcat tcaccgctga cgaactgtgg
241 gagtacctgc cgggcgagcg taacgagtcc gtcatgctca acacctggta cgaaggcctg
301 accgaactgc cggctgactt cgaactgggc cgcgagtact gggagggcgt gatggccgtc
361 aaggttgcgg tgaacaagga actggaagtc cagcgtgcgg ccaaggccgt gggtggcaac
421 ctgcaagccg aagtcaccct gtttgccgaa gacggcctga ccgccgacct ggccaagctg
481 agcaacgagc tgcgcttcgt gctgatcacc tctactgcga gcctggcgcc gtttacccag
541 gctccggcag atgcggtggc taccgaagtg cctggcctta agctcaaagt ggtcaagtcg
601 gccttcccca agtgcgctcg ttgctggcac tgccgtgaag acgtcggcgt gaaccctgag
661 cacccggaaa tctgcggtcg ttgtgtcgac aacatcagcg gtgaaggcga ggttcgccac
721 tatgcctaat gcagacagtc gtttcggacg tctgggctgg ctcgtactga gcctgctggt
781 cctggtcatt gaccaggtca gcaaggctca cttcgagggc tccctggaaa tgttccagca
841 aatcgtggtg atcccggatt atttcagctg gaccctggcc tacaacactg gcgccgcctt
901 cagcttcctc gctgacggcg gtggctggca gcgctggctg tttgctgtga tcgccgtggt
961 ggtaagtgcc gtactggtgg tgtggctcaa gcgcctgggc cgcgacgaca cctggctggc
1021 catcgcgctg gccctagtgt tgggtggcgc gctgggcaac ctgtatgacc gcatcgccct
1081 gggccatgtg atcgacttta tcctggtgca ttggcagaac cgccactact tcccggcgtt
1141 caactttgcc gacagtgcta tcaccgtcgg cgcaatcatg ctggcgctgg atatgttcaa
1201 gagcaagaaa accggagaaa ccgtcaatga ctgatcaggt attggctgag caacgcatcg
1261 gccagaacac ggaagtcact ttgcatttcg cactgcgcct ggagaatggc gacacggtcg
1321 acagcacgtt cgacaaggcc cccgcgacct tcaaggtcgg cgacggcaac ctgctgccgg
1381 gtttcgaagc ggcactgttc ggtttcaagg ccggtgacaa gcgcaccctg caaatcctgc
1441 cggaaaacgc ctttggccag cccaacccgc aaaacgtgca gatcatcccc cgttcgcagt
1501 tccagaacat ggacctgtcg gaaggcttgc tggtgatctt caatgatgcg gcgaacactg
1561 aattgcctgg cgtggtgaaa gcatttgatg acgcgcaagt gaccatcgac ttcaatcacc
1621 cgttggccgg caagacgttg acctttgacg tcgagattat cgacgtcaaa gcgctgtaac
1681 cgaccgtacg cggtctaaaa tgtgggaggg ggcttgctcc cgatagcggt atatcagcta
1741 aatgtgcaga ctgatatacc tatatcggga gcaagccccc tcccaccttt gatctccatt
1801 gtcggttgag tcagtgtcaa attgactcaa tatggctgca atacacgagg cacagcatgc
1861 aaatcaaact cgccaacccc cgtggcttct gcgccggcgt ggaccgggcg atcgaaatcg
1921 tcaatcgcgc cctggaagtc ttcgggccgc cgatttatgt gcgccatgaa gtcgtccata
1981 acaaatttgt ggtcgaagac ttgcgtgcgc gcggggcgat ctttgtcgaa gaactcgatc
2041 aggtgaaaga cgacgtgatc gtcatcttca gtgcccacgg tgtttcccag gctgtacgta
2101 ccgaagcggc gggccgtggc ctgaaggtgt tcgatgccac ctgcccactg gtgaccaagg
2161 tgcatatcga ggtggcgcgc tacagccgcg acggtcgtga gtgcatcctg atcggccacg
2221 ccggtcaccc ggangtagaa ggcaccatgg gccaatacga cgccagcaac ggcggtgcta
2281 tctacctggt cgaggacgag aaagacgtcg ccaacttgca ggtgcacaat ccggaacgcc
2341 tggccttcgt gacccagacc accttgtcca tggacgacac cagccgngta atcgat
//