GenBank-Updates@genbank.bio.net (12/20/90)
LOCUS SKTIGHA 1193 bp ss-mRNA VRT 120-DEC-1990
DEFINITION R.erinacea Ig rearranged H-chain mRNA (V-D-J-C region).
ACCESSION M29672 M35185
KEYWORDS constant region; diversity exon; immunoglobulin;
immunoglobulin heavy chain; joining exon; processed gene;
variable region; variable region subgroup VH-II.
SOURCE R.erinacea (Cx isotype) spleen, cDNA to mRNA, clone Re20.
ORGANISM Raja erinacea
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Chondrichthyes;
Elasmobranchii; Euselachii; Neoselachii; Batoidea; Rajiformes;
Rajidae.
REFERENCE 1 (bases 103 to 1193)
AUTHORS Harding,F.A., Amemiya,C.T., Litman,R.T., Cohen,N. and Litman,G.W.
TITLE Two disinctive immunoglobulin heavy chain isotypes in a primitive,
cartilaginous fish, Raja erinacea
JOURNAL Nucleic Acids Res. 18, 6369-6376 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1989)
10900 Roosevelt Blvd., St. Petersburg, FL 33716] kindly submitted
by G.W.Litman, 02-NOV-1989.
Data awaiting author review.
FEATURES Location/Qualifiers
CDS 43..1185
/note="Ig H-chain V-D-J-C-region precursor"
sig_peptide 46..102
/note="Ig H-chain signal peptide"
mat_peptide 103..1182
/note="Ig H-chain"
misc_recomb 384^385
/note="V-region end/D-region start"
misc_recomb 408^409
/note="D-region end/J-region start"
misc_recomb 453^454
/note="J-region end/C-region start"
BASE COUNT 305 a 304 c 309 g 275 t
ORIGIN
1 cccattcctg gagtgtccaa gtgtgtgtcc gtgctcagag tgatgggggt cgctgtttat
61 ctctgtctcc ttctgttctg tctgccaggc gttcgatccg cggtcgtgct gaatcagaaa
121 ccgaccgagg cggcaaagtc tggagagtcc ctcaaactga cctgtgtaac cagcgggttc
181 agcctcagca gctccaacgt gcattgggtg aaacaagtcc ccgggaaagg gctggagtgg
241 gtggcgatca tgtggtatga tgatgacaaa gattacgcgc ctgccttcag cggccgattc
301 actgtttcca gggacagcag caatgtctat ctccaaatga ccaacctgag tctggccgac
361 acggccacct attactgtgc ggcagccatg gggggctcta tatactggct tgagtactgg
421 ggtgcaggaa cctcgctgac agtgacttca gaggatgtgg ttttgccttc agtccacatc
481 acctcttcct gcaacacgga atctggccaa gagatcagca tcctctgtct ggtcaaggac
541 tacctgcctg aggtcatcag tcagacatgg tccaccagca gtggggtcat caacaatgga
601 ataacaaagt acccaccagt gttgggacaa aacaagaagt acacaatgag cagcttgctg
661 cgagtctctg tagcagattg gaacaggaaa acctactact gcaaggcagg gtacaagccg
721 gacaacatgg tgaaaacgga gatccagaag cctcaagccc cacagctcat cccccttgtt
781 ccatctccgg agactctcca caatcaaaca actgctgtcc tgggctgcat gatatctgga
841 ttctctcctg acaatattaa agtttcctgg aaaaaagctg gacttaatca agcgggcgtc
901 gttctcccat ccactccgag aactaacggt ggatttgaaa cagttgctta cctgccgttg
961 aatgtggagg aatggaccaa caaacaggaa tatacttgtg aagtgaccca cgcaccttcc
1021 ggcttcagcg acaagatcaa catgagatat caagagggtg gaaaatgtcc cggctgttcg
1081 aagtgtctgc cgaagttcat ctaccagagt aatctcaatg tgtcgttctc agatggttct
1141 acccagcagt atcattgttg ggcaggaaag tgtgaaataa agtaattggc tgc
//GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS SKTIGHA 1193 bp ss-mRNA VRT 22-DEC-1990
DEFINITION R.erinacea Ig rearranged H-chain mRNA (V-D-J-C region).
ACCESSION M29672 M35185
KEYWORDS constant region; diversity exon; immunoglobulin;
immunoglobulin heavy chain; joining exon; processed gene;
variable region; variable region subgroup VH-II.
SOURCE R.erinacea (Cx isotype) spleen, cDNA to mRNA, clone Re20.
ORGANISM Raja erinacea
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Chondrichthyes;
Elasmobranchii; Euselachii; Neoselachii; Batoidea; Rajiformes;
Rajidae.
REFERENCE 1 (bases 103 to 1193)
AUTHORS Harding,F.A., Amemiya,C.T., Litman,R.T., Cohen,N. and Litman,G.W.
TITLE Two disinctive immunoglobulin heavy chain isotypes in a primitive,
cartilaginous fish, Raja erinacea
JOURNAL Nucleic Acids Res. 18, 6369-6376 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1989)
10900 Roosevelt Blvd., St. Petersburg, FL 33716] kindly submitted
by G.W.Litman, 02-NOV-1989.
Data awaiting author review.
FEATURES Location/Qualifiers
CDS 43..1185
/note="Ig H-chain V-D-J-C-region precursor"
sig_peptide 46..102
/note="Ig H-chain signal peptide"
mat_peptide 103..1182
/note="Ig H-chain"
misc_recomb 384^385
/note="V-region end/D-region start"
misc_recomb 408^409
/note="D-region end/J-region start"
misc_recomb 453^454
/note="J-region end/C-region start"
BASE COUNT 305 a 304 c 309 g 275 t
ORIGIN
1 cccattcctg gagtgtccaa gtgtgtgtcc gtgctcagag tgatgggggt cgctgtttat
61 ctctgtctcc ttctgttctg tctgccaggc gttcgatccg cggtcgtgct gaatcagaaa
121 ccgaccgagg cggcaaagtc tggagagtcc ctcaaactga cctgtgtaac cagcgggttc
181 agcctcagca gctccaacgt gcattgggtg aaacaagtcc ccgggaaagg gctggagtgg
241 gtggcgatca tgtggtatga tgatgacaaa gattacgcgc ctgccttcag cggccgattc
301 actgtttcca gggacagcag caatgtctat ctccaaatga ccaacctgag tctggccgac
361 acggccacct attactgtgc ggcagccatg gggggctcta tatactggct tgagtactgg
421 ggtgcaggaa cctcgctgac agtgacttca gaggatgtgg ttttgccttc agtccacatc
481 acctcttcct gcaacacgga atctggccaa gagatcagca tcctctgtct ggtcaaggac
541 tacctgcctg aggtcatcag tcagacatgg tccaccagca gtggggtcat caacaatgga
601 ataacaaagt acccaccagt gttgggacaa aacaagaagt acacaatgag cagcttgctg
661 cgagtctctg tagcagattg gaacaggaaa acctactact gcaaggcagg gtacaagccg
721 gacaacatgg tgaaaacgga gatccagaag cctcaagccc cacagctcat cccccttgtt
781 ccatctccgg agactctcca caatcaaaca actgctgtcc tgggctgcat gatatctgga
841 ttctctcctg acaatattaa agtttcctgg aaaaaagctg gacttaatca agcgggcgtc
901 gttctcccat ccactccgag aactaacggt ggatttgaaa cagttgctta cctgccgttg
961 aatgtggagg aatggaccaa caaacaggaa tatacttgtg aagtgaccca cgcaccttcc
1021 ggcttcagcg acaagatcaa catgagatat caagagggtg gaaaatgtcc cggctgttcg
1081 aagtgtctgc cgaagttcat ctaccagagt aatctcaatg tgtcgttctc agatggttct
1141 acccagcagt atcattgttg ggcaggaaag tgtgaaataa agtaattggc tgc
//