GenBank-Updates@genbank.bio.net (12/20/90)
LOCUS HUMETMAGA 3343 bp ds-DNA PRI 120-DEC-1990
DEFINITION Human secreted epithelial tumor mucin antigen (H23Ag) gene,
complete cds.
ACCESSION M35093
KEYWORDS cell surface antigen; tumor mucin antigen.
SOURCE Human breast tumor cell line MCF7 DNA, clone lambda-gtWES.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 3343)
AUTHORS Tsarfaty,I., Hareuveni,M., Horev,J., Zaretsky,J., Weiss,M.,
Jeltsch,J.M., Garnier,J.M., Lathe,R., Keydar,I. and Wreschner,D.H.
TITLE Isolation and characterization of an expressed hypervariable gene
coding for a breast-cancer-associated antigen
JOURNAL Gene 93, 313-318 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Gene (1990) In
press] kindly submitted
by I.Tsarfaty, 12-JUN-1990.
FEATURES Location/Qualifiers
CDS join(785..842,1342..2207)
/product="tumor mucin antigen"
mat_peptide join(806..842,1342..2207)
/product="tumor mucin antigen"
misc_signal 384..397
/note="H23Ag ERE"
misc_signal 633..644
/note="H23Ag CACCT motifs"
misc_signal 689..692
/note="H23Ag TATA box"
precursor_RNA 777..>2207
/note="H23Ag mRNA and introns"
/gene="H23Ag"
exon 785..842
/note="secreted epithelial tumor mucin antigen precursor,
exon 1 (H23Ag)"
sig_peptide 785..805
/gene="tumor mucin antigen"
intron 843..1341
/note="H23Ag intron A"
misc_feature 1063..1090
/note="put. enhancer"
exon 1342..2207
/note="secreted epithelial tumor mucin antigen precursor,
exon 2 (H23Ag)"
repeat_region 1670..1729
/note="repeat unit"
BASE COUNT 679 a 986 c 981 g 697 t
ORIGIN Chromosome 1q21-q24.
1 gagctcctgg ccagtggtgg agagtggcaa ggaaggaccc tagggttcat cggagcccag
61 gtttactccc ttaagtggaa atttcttccc ccactcccct ccttggcttt ctccaaggag
121 ggaaccccag gctgctggaa agtccggctg gggcggggac tgtgggtttc agggtagaac
181 tgcgtgtgga acgggacagg gagcggttag aagggtgggg ctattccggg aagtggtggt
241 ggggggaggg agcccaaaac tagcacctag tccactcatt atccagccct cttatttctc
301 ggccgcctct gcttcagtgg acccggggag ggcggggaag tggagtggga gacctagggg
361 tgggcttccc gaccttgctg tacaggacct cgacctagct ggctttgttc cccatcccca
421 gttagttgtt gccctgaggc taaaactaga gcccaggggc cccaagttcc agactgcccc
481 tcccccctcc cccggagcca gggagtggtt ggtgaaaggg ggaggccagc tggagaagaa
541 acgggtagtc aggggttgca gcattagagc ccttgtagcc ctagcccagg aatggttgga
601 gagagaagag tagagtaggg aggggggttt gtcacctgtc acctgctcgg ctgtgcctag
661 ggcgggcggg ggggagtggg gggaccggta taaagcggta ggcgcctgtg cccgctccac
721 ctctcaagca gccagcgcct gcctgaatct gttctgcccc ctccccaccc atttcaccac
781 caccatgaca ccgggcaccc agtctccttt cttcctgctg ctgctcctca cagtgcttac
841 aggtgagggg cacgaggtgg ggagtgggct gccctgctta ggtggtcttc gtggtctttc
901 tgtgggtttt gctccctggc agatggcacc agaagttaag gtaagaattg cagacagagg
961 ctgccctgtc tgtgccagaa ggagggagag gctaaggaca ggctgagaag agttgccccc
1021 aaccctgaga gtgggtacca ggggcaagca aatgtcctgt agagaagtct agggggaaga
1081 gagtagggag agggaaggct taagagggga agaaatgcag gggccatgag ccaaggccta
1141 tgggcagaga gaaggaggct gctgcaggaa ggaggcggcc aacccagggg ttactgaggc
1201 tgcccactcc ccagtcctcc tggtattatt tctctggtgg ccaggcttat attttcttct
1261 tgctcttatt tttccttcat aaagacccaa ccctatgact ttaacttctt acagctacca
1321 cagcccctgg gcccgcaaca gttgttacag gttctggtca tgcaagctct accccaggtg
1381 gagaaaagga gacttcggct acccagagaa gttcagtgcc cagctctact gagaagaatg
1441 ctgtgagtat gaccagcagc gtactctcca gccacagccc cggttcaggc tcctccacca
1501 ctcagggaca ggatgtcact ctggccccgg ccacggaacc agcttcaggt tcagctgcca
1561 cctggggaca ggatgtcacc tcggtcccag tcaccaggcc agccctgggc tccaccaccc
1621 cgccagccca cgatgtcacc tcagccccgg acaacaagcc agccccgggc tccaccgccc
1681 ccccagccca gggtgtcacc tcggccccgg agaccaggcc gcccccgggc tccaccgccc
1741 ccccagccca tggtgtcacc tcggcgccgg acaacaggcc cgccttggcg tccaccgccc
1801 ctccagtcca caatgtcacc tcggcctcag gctctgcatc aggctcagct tctactctgg
1861 tgcacaacgg cacctctgcc agggctacca caaccccagc cagcaagagc actccattct
1921 caattcccag ccaccactct gatactccta ccacccttgc cagccatagc accaagactg
1981 atgccagtag cactcaccat agcacggtac ctcctctcac ctcctccaat cacagcactt
2041 ctccccagtt gtctactggg gtctctttct ttttcctgtc ttttcacatt tcaaacctcc
2101 agtttaattc ctctctggaa gatcccagca ccgactacta ccaagagctg cagagagaca
2161 tttctgaaat ggtgagtatc ggcctttcct tccccatgct cccctgaagc agccatcaga
2221 actgtccaca ccctttgcat caagcctgag tcctttccct ctcaccccag tttttgcaga
2281 tttataaaca agggggtttt ctgggcctct ccaatattaa gttcaggtac agttctgggt
2341 gtggacccag tgtggtggtt ggaggggtgg gtggtggtca tgagccgtag ggagggactg
2401 gtgcacttaa ggttggggga agagtgctga gccagagctg ggacccgtgg ctgaagtgcc
2461 catttccctg tgaccaggcc aggatctgtg gtggtacaat tgactctggc cttccgagaa
2521 ggtaccatca atgtccacga cgtggagaca cagttcaatc agtataaaac ggaagcagcc
2581 tctcgatata acctgacgat ctcaagacgt cagcggtgag gctacttccc tgctgcagcc
2641 agcaccatgc cggggcccct ctccttccag tgtctgggtc cccgctcttt ccttagtgct
2701 ggcagcggga ggggcgcctc ctctgggaga ctgccctgac cactgctttt ccttttagtg
2761 agtgatgtgc catttccttt ctctgaccag tctggggctg gggtgccagg ctggggcatc
2821 gcgctgctgg tgctggtctg tgttctggtt gcgctggcca ttgtctatct cattgccttg
2881 gtgagtgcag tccctggccc tgatcagagc cccccggtag aaggcactcc atggcctgcc
2941 ataacctcct atctccccag gctgtctgtc agtgccgccg aaagaactac gggcagctgg
3001 acatctttcc agcccgggat acctaccatc ctatgagcga gtaccccacc taccacaccc
3061 atgggcgcta tgtgccccta gcagtaccga tcgtagcccc tatgagaagg tgagattggg
3121 ccccacaggc aggggaagca gagggtttgg ctgggcaagg attctgaagg gggtacttgg
3181 aaaacccaaa gagcttggaa gaggtgagaa gtggcgtgaa gtgagcaggg gagggctggc
3241 aaggatgagg ggcagaggtc agaggagttt tgggggacag gcctgggagg agactatgga
3301 agaaaggggc ccctcaaaag ggagtgcccc actgccagaa ttc
//GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS HUMETMAGA 3343 bp ds-DNA PRI 22-DEC-1990
DEFINITION Human secreted epithelial tumor mucin antigen (H23Ag) gene,
complete cds.
ACCESSION M35093
KEYWORDS cell surface antigen; tumor mucin antigen.
SOURCE Human breast tumor cell line MCF7 DNA, clone lambda-gtWES.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 3343)
AUTHORS Tsarfaty,I., Hareuveni,M., Horev,J., Zaretsky,J., Weiss,M.,
Jeltsch,J.M., Garnier,J.M., Lathe,R., Keydar,I. and Wreschner,D.H.
TITLE Isolation and characterization of an expressed hypervariable gene
coding for a breast-cancer-associated antigen
JOURNAL Gene 93, 313-318 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Gene (1990) In
press] kindly submitted
by I.Tsarfaty, 12-JUN-1990.
FEATURES Location/Qualifiers
CDS join(785..842,1342..2207)
/product="tumor mucin antigen"
mat_peptide join(806..842,1342..2207)
/product="tumor mucin antigen"
misc_signal 384..397
/note="H23Ag ERE"
misc_signal 633..644
/note="H23Ag CACCT motifs"
misc_signal 689..692
/note="H23Ag TATA box"
precursor_RNA 777..>2207
/note="H23Ag mRNA and introns"
/gene="H23Ag"
exon 785..842
/note="secreted epithelial tumor mucin antigen precursor,
exon 1 (H23Ag)"
sig_peptide 785..805
/gene="tumor mucin antigen"
intron 843..1341
/note="H23Ag intron A"
misc_feature 1063..1090
/note="put. enhancer"
exon 1342..2207
/note="secreted epithelial tumor mucin antigen precursor,
exon 2 (H23Ag)"
repeat_region 1670..1729
/note="repeat unit"
BASE COUNT 679 a 986 c 981 g 697 t
ORIGIN Chromosome 1q21-q24.
1 gagctcctgg ccagtggtgg agagtggcaa ggaaggaccc tagggttcat cggagcccag
61 gtttactccc ttaagtggaa atttcttccc ccactcccct ccttggcttt ctccaaggag
121 ggaaccccag gctgctggaa agtccggctg gggcggggac tgtgggtttc agggtagaac
181 tgcgtgtgga acgggacagg gagcggttag aagggtgggg ctattccggg aagtggtggt
241 ggggggaggg agcccaaaac tagcacctag tccactcatt atccagccct cttatttctc
301 ggccgcctct gcttcagtgg acccggggag ggcggggaag tggagtggga gacctagggg
361 tgggcttccc gaccttgctg tacaggacct cgacctagct ggctttgttc cccatcccca
421 gttagttgtt gccctgaggc taaaactaga gcccaggggc cccaagttcc agactgcccc
481 tcccccctcc cccggagcca gggagtggtt ggtgaaaggg ggaggccagc tggagaagaa
541 acgggtagtc aggggttgca gcattagagc ccttgtagcc ctagcccagg aatggttgga
601 gagagaagag tagagtaggg aggggggttt gtcacctgtc acctgctcgg ctgtgcctag
661 ggcgggcggg ggggagtggg gggaccggta taaagcggta ggcgcctgtg cccgctccac
721 ctctcaagca gccagcgcct gcctgaatct gttctgcccc ctccccaccc atttcaccac
781 caccatgaca ccgggcaccc agtctccttt cttcctgctg ctgctcctca cagtgcttac
841 aggtgagggg cacgaggtgg ggagtgggct gccctgctta ggtggtcttc gtggtctttc
901 tgtgggtttt gctccctggc agatggcacc agaagttaag gtaagaattg cagacagagg
961 ctgccctgtc tgtgccagaa ggagggagag gctaaggaca ggctgagaag agttgccccc
1021 aaccctgaga gtgggtacca ggggcaagca aatgtcctgt agagaagtct agggggaaga
1081 gagtagggag agggaaggct taagagggga agaaatgcag gggccatgag ccaaggccta
1141 tgggcagaga gaaggaggct gctgcaggaa ggaggcggcc aacccagggg ttactgaggc
1201 tgcccactcc ccagtcctcc tggtattatt tctctggtgg ccaggcttat attttcttct
1261 tgctcttatt tttccttcat aaagacccaa ccctatgact ttaacttctt acagctacca
1321 cagcccctgg gcccgcaaca gttgttacag gttctggtca tgcaagctct accccaggtg
1381 gagaaaagga gacttcggct acccagagaa gttcagtgcc cagctctact gagaagaatg
1441 ctgtgagtat gaccagcagc gtactctcca gccacagccc cggttcaggc tcctccacca
1501 ctcagggaca ggatgtcact ctggccccgg ccacggaacc agcttcaggt tcagctgcca
1561 cctggggaca ggatgtcacc tcggtcccag tcaccaggcc agccctgggc tccaccaccc
1621 cgccagccca cgatgtcacc tcagccccgg acaacaagcc agccccgggc tccaccgccc
1681 ccccagccca gggtgtcacc tcggccccgg agaccaggcc gcccccgggc tccaccgccc
1741 ccccagccca tggtgtcacc tcggcgccgg acaacaggcc cgccttggcg tccaccgccc
1801 ctccagtcca caatgtcacc tcggcctcag gctctgcatc aggctcagct tctactctgg
1861 tgcacaacgg cacctctgcc agggctacca caaccccagc cagcaagagc actccattct
1921 caattcccag ccaccactct gatactccta ccacccttgc cagccatagc accaagactg
1981 atgccagtag cactcaccat agcacggtac ctcctctcac ctcctccaat cacagcactt
2041 ctccccagtt gtctactggg gtctctttct ttttcctgtc ttttcacatt tcaaacctcc
2101 agtttaattc ctctctggaa gatcccagca ccgactacta ccaagagctg cagagagaca
2161 tttctgaaat ggtgagtatc ggcctttcct tccccatgct cccctgaagc agccatcaga
2221 actgtccaca ccctttgcat caagcctgag tcctttccct ctcaccccag tttttgcaga
2281 tttataaaca agggggtttt ctgggcctct ccaatattaa gttcaggtac agttctgggt
2341 gtggacccag tgtggtggtt ggaggggtgg gtggtggtca tgagccgtag ggagggactg
2401 gtgcacttaa ggttggggga agagtgctga gccagagctg ggacccgtgg ctgaagtgcc
2461 catttccctg tgaccaggcc aggatctgtg gtggtacaat tgactctggc cttccgagaa
2521 ggtaccatca atgtccacga cgtggagaca cagttcaatc agtataaaac ggaagcagcc
2581 tctcgatata acctgacgat ctcaagacgt cagcggtgag gctacttccc tgctgcagcc
2641 agcaccatgc cggggcccct ctccttccag tgtctgggtc cccgctcttt ccttagtgct
2701 ggcagcggga ggggcgcctc ctctgggaga ctgccctgac cactgctttt ccttttagtg
2761 agtgatgtgc catttccttt ctctgaccag tctggggctg gggtgccagg ctggggcatc
2821 gcgctgctgg tgctggtctg tgttctggtt gcgctggcca ttgtctatct cattgccttg
2881 gtgagtgcag tccctggccc tgatcagagc cccccggtag aaggcactcc atggcctgcc
2941 ataacctcct atctccccag gctgtctgtc agtgccgccg aaagaactac gggcagctgg
3001 acatctttcc agcccgggat acctaccatc ctatgagcga gtaccccacc taccacaccc
3061 atgggcgcta tgtgccccta gcagtaccga tcgtagcccc tatgagaagg tgagattggg
3121 ccccacaggc aggggaagca gagggtttgg ctgggcaagg attctgaagg gggtacttgg
3181 aaaacccaaa gagcttggaa gaggtgagaa gtggcgtgaa gtgagcaggg gagggctggc
3241 aaggatgagg ggcagaggtc agaggagttt tgggggacag gcctgggagg agactatgga
3301 agaaaggggc ccctcaaaag ggagtgcccc actgccagaa ttc
//