GenBank-Updates@genbank.bio.net (12/20/90)
LOCUS ECOXERC 2500 bp ds-DNA BCT 120-DEC-1990
DEFINITION Escherichia coli lambda-integrase (XerC) gene, complete cds,
diaminopimelate (dapF), 3' end, and helicase II (uvrD) gene, 5'
end.
ACCESSION M38257
KEYWORDS DNA-dependent ATPase; dapF gene; diaminopimelate; helicase II;
lambda-integrase; recombinase; uvrD gene; xerC gene.
SOURCE E.coli K12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2500)
AUTHORS Colloms,S.D., Sykora,P., Szatmari,G. and Sherratt,D.J.
TITLE Recombination at ColE1 cer requires the Escherichia coli xerC gene
product, a member of the lambda integrase family of site- specific
recombinases
JOURNAL J. Bacteriol. 172, 6973-6980 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by S.D.Colloms, 04-SEP-1990.
Institute of Genetics
University of Glasgow
Church Street, Glasgow
G11 5JS, Scotland
FEATURES Location/Qualifiers
CDS <1..51
/gene="dapF"
/codon_start=1
/product="diaminopimelate"
CDS 48..755
/gene="ORF 235"
/codon_start=48
CDS 752..1648
/gene="xerC"
/codon_start=752
/product="lambda-integrase"
CDS 1648..2364
/gene="ORF 238"
/codon_start=1648
promoter 2338..2365
/number=1
/gene="uvrD"
promoter 2407..2432
/number=2
/gene="uvrD"
CDS 2448..>2500
/gene="uvrD"
/codon_start=2448
/product="helicase II"
BASE COUNT 548 a 649 c 722 g 581 t
ORIGIN
1 tatatgactg gcccggcggt acatgtctac gacggattta ttcatctatg aagcaaccag
61 gggaagaact gcaggaaaca ctcacggagc ttgatgaccg ggcggttgtc gattatctga
121 ttaaaaatcc tgagtttttt atccgtaatg cgcgcgcagt agaagcgata cgtgtgccgc
181 atccggtacg cggcaccgtt tcgttggtcg agtggcacat ggcccgcgca cgtaatcata
241 ttcatgttct ggaagagaac atggcgctgt tgatggaaca ggctatcgcc aacgaaggcc
301 tgttttatcg cctactctac ctgcagcgca gtctcaccgc cgccagcagt ctcgacgata
361 tgctgatgcg ctttcaccgc tgggcgcgcg atctcggcct ggcaggtgcg agtctgcgcc
421 tgtttccgga tcgctggcgc ttaggtgcgc cgtcgaacca cactcatctg gcattaagcc
481 gtcagtcttt cgaaccgctg cgtattcagc gtttggggca ggaacagcac tatcttgggc
541 cgcttaacgg accagagctg ctggtggtgc taccggaagc gaaagcggtg ggatcggtgg
601 cgatgtcgat gctgggaagc gatgctgatt tgggtgtcgt gctgtttacc agtcgcgatg
661 ccagtcacta tcaacaaggg caaggaacgc agttacttca tgaaattgcg ctgatgttgc
721 cggagcttct ggagcgttgg attgaacgcg tatgaccgat ttacacaccg atgtagaacg
781 ctacctacgt tatctgagcg tggagcgcca gcttagcccg ataaccctgc ttaactacca
841 gcgtcagctt gaggcgatca tcaattttgc cagcgaaaac ggcctgcaaa gctggcagca
901 atgtgatgtg acgatggtgc gcaattttgc tgtacgcagt cgccgtaaag ggctgggagc
961 agcaagtctg gcgttacggc tttctgcgct acgtagcttt tttgactggc tggtcagcca
1021 gaacgaactc aaagctaacc cggcgaaagg tgtttcggca ccgaaagcgc cgcgtcatct
1081 gccgaaaaac atcgacgtcg acgatatgaa tcggctgctg gatattgata tcaatgatcc
1141 cctcgctgta cgcgaccgtg caatgctgga agtgatgtac ggcgcgggtc tgcgtctttc
1201 tgagctggtg gggctggata ttaaacacct cgacctggag tctggtgaag tgtgggttat
1261 ggggaaaggc agcaaagagc gccgcctgcc gattggtcgc aacgctgtgg cgtggattga
1321 gcactggctt gatttgcgcg acctgtttgg tagcgaagac gacgcgcttt ttctgtcgaa
1381 actgggcaag cgtatctccg cgcgtaatgt gcagaaacgc tttgccgaat ggggcataaa
1441 acaagggctg aataatcacg ttcatccgca taaattacgt cactcgttcg ccacgcatat
1501 gctggagtcg agcggcgatc ttcgtggtgt gcaggagctg ctgggtcatg ccaacctctc
1561 caccacgcaa atctatactc atcttgattt tcaacacctt gcctcggtgt acgatgcggc
1621 gcatccacgc gccaaacggg ggaaataatg cgtttttacc ggcctttggg gcgcatctcg
1681 gcgctcacct ttgacctgga tgataccctt tacgataacc gtccggtgat tttgcgcacc
1741 gagcgagagg cgcttacctt tgtgcaaaat tatcatccgg cgctgcgcag cttccagaat
1801 gaagatctgc aacgcctgcg ccaggcggta cgggaagcgg aacccgagat ttatcacgac
1861 gtgacgcgct ggcgttttcg ttcgattgaa caagcgatgc tcgacgccgg gctgagtgcc
1921 gaagaagcca gtgcaggcgc acacgcagca atgatcaact ttgccaaatg gcgcagccga
1981 atcgacgtcc cgcagcaaac tcacgacacc ttaaaacagc tggcgaagaa atggccgctg
2041 gtggcgatca ccaacggtaa cgcccagccg gagctgtttg gtttggggga ttattttgag
2101 tttgtgctgc gcgctggccc gcacgggcgc tcaaaaccgt tcagcgatat gtactttttg
2161 gctgcggaaa aactcaacgt gccgatcggc gagatcttac atgttgggga cgatctcacc
2221 actgacgtgg gtggggcaat tcgcaccgga atgcaggctt gttggatcag accggaaaat
2281 ggcgatctga tgcaaacctg ggacagccgt ttactgccgc atctggaaat ttcccggttg
2341 gcatctctga cctcgctgat ataatcagca aatctgtata tatacccagc tttttggcgg
2401 agggcgttgc gcttctccgc ccaacctatt tttacgcggc ggtgccaatg gacgtttctt
2461 acctgctcga cagccttaat gacaaacagc gcgaagcggt
//GenBank-Updates@genbank.bio.net (12/22/90)
LOCUS ECOXERC 2500 bp ds-DNA BCT 22-DEC-1990
DEFINITION Escherichia coli lambda-integrase (XerC) gene, complete cds,
diaminopimelate (dapF), 3' end, and helicase II (uvrD) gene, 5'
end.
ACCESSION M38257
KEYWORDS DNA-dependent ATPase; dapF gene; diaminopimelate; helicase II;
lambda-integrase; recombinase; uvrD gene; xerC gene.
SOURCE E.coli K12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2500)
AUTHORS Colloms,S.D., Sykora,P., Szatmari,G. and Sherratt,D.J.
TITLE Recombination at ColE1 cer requires the Escherichia coli xerC gene
product, a member of the lambda integrase family of site- specific
recombinases
JOURNAL J. Bacteriol. 172, 6973-6980 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by S.D.Colloms, 04-SEP-1990.
Institute of Genetics
University of Glasgow
Church Street, Glasgow
G11 5JS, Scotland
FEATURES Location/Qualifiers
CDS <1..51
/gene="dapF"
/codon_start=1
/product="diaminopimelate"
CDS 48..755
/gene="ORF 235"
/codon_start=48
CDS 752..1648
/gene="xerC"
/codon_start=752
/product="lambda-integrase"
CDS 1648..2364
/gene="ORF 238"
/codon_start=1648
promoter 2338..2365
/number=1
/gene="uvrD"
promoter 2407..2432
/number=2
/gene="uvrD"
CDS 2448..>2500
/gene="uvrD"
/codon_start=2448
/product="helicase II"
BASE COUNT 548 a 649 c 722 g 581 t
ORIGIN
1 tatatgactg gcccggcggt acatgtctac gacggattta ttcatctatg aagcaaccag
61 gggaagaact gcaggaaaca ctcacggagc ttgatgaccg ggcggttgtc gattatctga
121 ttaaaaatcc tgagtttttt atccgtaatg cgcgcgcagt agaagcgata cgtgtgccgc
181 atccggtacg cggcaccgtt tcgttggtcg agtggcacat ggcccgcgca cgtaatcata
241 ttcatgttct ggaagagaac atggcgctgt tgatggaaca ggctatcgcc aacgaaggcc
301 tgttttatcg cctactctac ctgcagcgca gtctcaccgc cgccagcagt ctcgacgata
361 tgctgatgcg ctttcaccgc tgggcgcgcg atctcggcct ggcaggtgcg agtctgcgcc
421 tgtttccgga tcgctggcgc ttaggtgcgc cgtcgaacca cactcatctg gcattaagcc
481 gtcagtcttt cgaaccgctg cgtattcagc gtttggggca ggaacagcac tatcttgggc
541 cgcttaacgg accagagctg ctggtggtgc taccggaagc gaaagcggtg ggatcggtgg
601 cgatgtcgat gctgggaagc gatgctgatt tgggtgtcgt gctgtttacc agtcgcgatg
661 ccagtcacta tcaacaaggg caaggaacgc agttacttca tgaaattgcg ctgatgttgc
721 cggagcttct ggagcgttgg attgaacgcg tatgaccgat ttacacaccg atgtagaacg
781 ctacctacgt tatctgagcg tggagcgcca gcttagcccg ataaccctgc ttaactacca
841 gcgtcagctt gaggcgatca tcaattttgc cagcgaaaac ggcctgcaaa gctggcagca
901 atgtgatgtg acgatggtgc gcaattttgc tgtacgcagt cgccgtaaag ggctgggagc
961 agcaagtctg gcgttacggc tttctgcgct acgtagcttt tttgactggc tggtcagcca
1021 gaacgaactc aaagctaacc cggcgaaagg tgtttcggca ccgaaagcgc cgcgtcatct
1081 gccgaaaaac atcgacgtcg acgatatgaa tcggctgctg gatattgata tcaatgatcc
1141 cctcgctgta cgcgaccgtg caatgctgga agtgatgtac ggcgcgggtc tgcgtctttc
1201 tgagctggtg gggctggata ttaaacacct cgacctggag tctggtgaag tgtgggttat
1261 ggggaaaggc agcaaagagc gccgcctgcc gattggtcgc aacgctgtgg cgtggattga
1321 gcactggctt gatttgcgcg acctgtttgg tagcgaagac gacgcgcttt ttctgtcgaa
1381 actgggcaag cgtatctccg cgcgtaatgt gcagaaacgc tttgccgaat ggggcataaa
1441 acaagggctg aataatcacg ttcatccgca taaattacgt cactcgttcg ccacgcatat
1501 gctggagtcg agcggcgatc ttcgtggtgt gcaggagctg ctgggtcatg ccaacctctc
1561 caccacgcaa atctatactc atcttgattt tcaacacctt gcctcggtgt acgatgcggc
1621 gcatccacgc gccaaacggg ggaaataatg cgtttttacc ggcctttggg gcgcatctcg
1681 gcgctcacct ttgacctgga tgataccctt tacgataacc gtccggtgat tttgcgcacc
1741 gagcgagagg cgcttacctt tgtgcaaaat tatcatccgg cgctgcgcag cttccagaat
1801 gaagatctgc aacgcctgcg ccaggcggta cgggaagcgg aacccgagat ttatcacgac
1861 gtgacgcgct ggcgttttcg ttcgattgaa caagcgatgc tcgacgccgg gctgagtgcc
1921 gaagaagcca gtgcaggcgc acacgcagca atgatcaact ttgccaaatg gcgcagccga
1981 atcgacgtcc cgcagcaaac tcacgacacc ttaaaacagc tggcgaagaa atggccgctg
2041 gtggcgatca ccaacggtaa cgcccagccg gagctgtttg gtttggggga ttattttgag
2101 tttgtgctgc gcgctggccc gcacgggcgc tcaaaaccgt tcagcgatat gtactttttg
2161 gctgcggaaa aactcaacgt gccgatcggc gagatcttac atgttgggga cgatctcacc
2221 actgacgtgg gtggggcaat tcgcaccgga atgcaggctt gttggatcag accggaaaat
2281 ggcgatctga tgcaaacctg ggacagccgt ttactgccgc atctggaaat ttcccggttg
2341 gcatctctga cctcgctgat ataatcagca aatctgtata tatacccagc tttttggcgg
2401 agggcgttgc gcttctccgc ccaacctatt tttacgcggc ggtgccaatg gacgtttctt
2461 acctgctcga cagccttaat gacaaacagc gcgaagcggt
//