GenBank-Updates@genbank.bio.net (01/10/91)
LOCUS PHSG664CG 2706 bp ds-DNA SYN 10-JAN-1991
DEFINITION Synthetic cloning vector plasmid pHSG664, complete genome.
ACCESSION M12787
KEYWORDS beta-lactamase; complete genome; lactamase; ribosomal protein;
ribosomal protein S12; rpsL gene.
SOURCE Clones pUC9 (Ap-R gene region) and pNO1523 (strA+ gene) DNA, from
E.coli K12, clone pHSG684.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 2706)
AUTHORS Hashimoto-Gotoh,T., Kume,A., Masahashi,W., Takeshita,S. and
Fukuda,A.
TITLE Improved vector, pHSG664, for direct streptomycin-resistance
selection: cDNA cloning with G: C-tailing procedure and subcloning
for double-digest DNA fragments
JOURNAL Gene 41, 125-128 (1986)
STANDARD full staff_review
COMMENT Draft entry and sequence in computer readable form for [Gene 41,
125-128 (1986)] kindly
provided by R.Kikuno, 12-AUG-1986.
The cloning vector reported by [Gene 41, 125-128 (1986)] contains
ten unique restriction
sites: EcoRI, HpaI, PvuII, SphI, PstI, SmaI, BamHI, SalI, XbaI and
HindIII.
FEATURES Location/Qualifiers
CDS 232..606
/product="ribosomal protein S12"
CDS complement(1712..2572)
/product="beta-lactamase"
BASE COUNT 684 a 681 c 646 g 695 t
ORIGIN EcoRI site.
1 aattcccggt ttgactggtc aaatttcgaa cgacatcatt ctcattgact atactgattt
61 cgtcagactt acggttaagc accccagcca gatggcctgg tgatgatggc gggatcgttg
121 tatatttctt gacacctttt cggcatcgcc ctaaaattcg gcgtcctcat attgtgtgag
181 gacgttttat tacgtgttta cgaagcaaaa gctaaaacca ggagctattt aatggcaaca
241 gttaaccagc tggtacgcaa accacgtgct cgcaaagttg cgaaaagcaa cgtgcctgcg
301 ctggaagcat gcccgcaaaa acgtggcgta tgtactcgtg tatatactac cactcctaaa
361 aaaccgaact ccgcgctgcg taaagtatgc cgtgttcgtc tgactaacgg tttcgaagtg
421 acttcctaca tcggtggtga aggtcacaac ctgcaggagc actccgtgat cctgatccgt
481 ggcggtcgtg ttaaagacct cccgggtgtt cgttaccaca ccgtacgtgg tgcgcttgac
541 tgctccggcg ttaaagaccg taagcaggct cgttccaagt atggcgtgaa gcgtcctaag
601 gcttaatggt tctccgttaa gtaaggccaa acgttttaac ttaaatgtca aactaaactc
661 gtagagtttt ggacaatcct gaattaacaa cggagtattt ccatgccacg tcgtcgcgtc
721 attggtcagc gtaaaattct gccggatccg tcgaccgatc tctagaagct tcttccgctt
781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
1021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
1081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
1141 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
1201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
1261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
1321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
1381 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
1441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
1501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
1561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
1621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
1681 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
1741 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
1801 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
1861 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
1921 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
1981 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
2041 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
2101 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
2161 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
2221 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
2281 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
2341 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
2401 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
2461 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
2521 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
2581 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
2641 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
2701 ctgacg
//