GenBank-Updates@genbank.bio.net (01/10/91)
LOCUS RABIGHVDJA 770 bp ds-DNA MAM 10-JAN-1991
DEFINITION Rabbit rearranged Ig heavy chain gene VDJ-region, clone PCR4-1.
ACCESSION M55381
KEYWORDS diversity exon; immunoglobulin heavy chain; joining exon;
processed gene; variable region.
SOURCE Rabbit adult spleen and mesenteric lymph node DNA, clone PCR4-1.
ORGANISM Oryctolagus cuniculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Lagomorpha; Leporidae.
REFERENCE 1 (bases 1 to 770)
AUTHORS Becker,R.S. and Knight,K.L.
TITLE Somatic diversification of immunoglobulin heavy chain VDJ genes:
Evidence for somatic gene conversion in rabbits
JOURNAL Cell 63, 987-997 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(236..281,384..770)
/gene="IgH"
/partial
/product="Ig heavy chain"
sig_peptide join(236..281,384..394)
/gene="IgH"
misc_feature 1..29
/note="5' PCR primer"
intron 282..383
/gene="IgH"
exon 384..770
/number=2
/gene="IgH"
/partial
mat_peptide 395..770
/gene="IgH"
/codon_start=395
/partial
/product="Ig heavy chain"
misc_feature 743..770
/note="3' PCR primer"
BASE COUNT 161 a 227 c 218 g 164 t
ORIGIN
1 gaattcctat cacagccatc actctacata cccaggacac cacatctgcc ctgggccctc
61 tcctgtctga ggcgtctacc tcatgcctgc tatatagggg cagcacatgc aaatagggcc
121 tccctctgcc catgaaaacc agcccagccc tcaccctgca gctctggcac aggagctcca
181 gccccagcac tcccaggtgt ccactcagtg atcgcactca acacagacac tcaccatgga
241 gactgggctg cgctggcttc tcctggtcgc tgtgctcaaa ggtaatgatg gagaacgcgg
301 ggcactgagt ctgggagagg acgtgagtga gagacacaga cagtgtgagt gacagtacct
361 gaccatgtcg tctgtgtttg caggtgtcca gtgtcagtcg ttggaggagt ccgggggaga
421 cctggtcaag cctggggcat ccctgacact cacctgcaca gcctctggat tctccttcag
481 cagcagcgct tacatgtgct gggtccgcca ggctccaggg aaggggctgg agtggatcgc
541 atgcattgct gctggtagta gtggtagcac ttactacgcg agctgggcga aaggccgatt
601 caccatctcc aaaacctcgt cgaccacggt gactctgcaa atgaccagtc tgacagccgc
661 ggacacggcc acctatttct gtgcgaaaag atttggtagt ggtagtggta gtggtgtcta
721 ctggggtttt aacttgtggg gcccaggcac cctggtcacc gtctcctcag
//