GenBank-Updates@genbank.bio.net (01/10/91)
LOCUS RABIGHVDJE 746 bp ds-DNA MAM 10-JAN-1991
DEFINITION Rabbit rearranged Ig heavy chain gene VDJ-region, clone PCR4-2.
ACCESSION M55385
KEYWORDS diversity exon; immunoglobulin heavy chain; joining exon;
processed gene; variable region.
SOURCE Rabbit spleen DNA, clone PCR4-2.
ORGANISM Oryctolagus cuniculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Lagomorpha; Leporidae.
REFERENCE 1 (bases 1 to 746)
AUTHORS Becker,R.S. and Knight,K.L.
TITLE Somatic diversification of immunoglobulin heavy chain VDJ genes:
Evidence for somatic gene conversion in rabbits
JOURNAL Cell 63, 987-997 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
sig_peptide join(236..281,384..394)
/gene="IgH"
CDS join(236..281,384..746)
/gene="IgH"
/partial
/product="Ig heavy chain"
misc_feature 1..29
/note="5' PCR primer"
intron 282..383
/gene="IgH"
exon 384..746
/number=2
/gene="IgH"
/partial
mat_peptide 395..746
/gene="IgH"
/codon_start=395
/partial
/product="Ig heavy chain"
misc_feature 722..746
/note="3' PCR primer"
BASE COUNT 160 a 220 c 212 g 154 t
ORIGIN
1 gaattcctat cacagccatc actctacata cccaggacac cacatctgcc ctgggccctc
61 tcctgtctga ggcgtctacc tcatgcctgc tatatagggg cagcacatgc agatagggcc
121 tccctctgcc catgaaaacc agcccagccc tcaccctgca gctctggcac aggagctcca
181 gccccagcac tcccaggtgt ccactcagtg atcgcactca acacagacac tcaccatgga
241 gactgggctg cgctggcttc tcctggtcgc tgtgctcaaa ggtaatgatg gagaacgcgg
301 ggcactgagt ctgggagagg acgtgagtga gagacacaga cagtgtgagt gacagtacct
361 gaccatgtcg tctgtgtttg caggtgtcca gtgtcagtcg ttggaggagt ccgggggagg
421 cctggtcaag cctgagggat ccctgacact cacctgcaaa gcctctggat tcgacctcag
481 tagctactac tacatgtgct gggtccgcca ggctccaggg aaggggctgg agtggatcgg
541 atgcattaat actggtagtg gtagcacatg gtacgcgagc tgggtgaatg gccgattcac
601 catctccaaa acctcgtcga ccacggtgac tctgcaaatg accagtctga cagccgcgga
661 cacggccacc tatttctgtg cgagagataa tggtgctagt ggtgctgact tgtggggccc
721 aggcaccctg gtcaccgtct cctcag
//