GenBank-Updates@genbank.bio.net (01/11/91)
LOCUS MUSADAM01 2308 bp ds-DNA ROD 11-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 1 (non-coding).
ACCESSION M34242 J04767
KEYWORDS adenosine deaminase.
SEGMENT 1 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2308)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
precursor_RNA 885..>2308
/note="adenosine deaminase (ADA) mRNA and introns"
intron 1008..>2308
/note="ADA intron A"
BASE COUNT 479 a 615 c 698 g 516 t
ORIGIN Chromosome 2.
1 cccacctcaa ggtgcgcaca agttacttaa ggaacttgct acaatatagc cctgctcccg
61 cccccaaaat cccaccaaac ctagagtatg gttctaaaca gctcacctgt taagtctcct
121 tggccaatcc tctagaagtt gaccatagta tgaagttttc tgcagcgtag tttttttctg
181 cccccctttc actactgtgt ctgagcacat gtgctgtgct ttgtagctga aactggcttt
241 attgctgcag aaaccagtcc actgtattta cccacagcac tgatgtgagc attctaaata
301 catctcgatg cgtgggcata tttatccagc gtaactgccc caggagagat gaactgtgtg
361 ttcctgtcca ccccctgtat cagcacctga gactagtctc agagtctctc tcacacacaa
421 cagtgttctc tgcatcccac ccgccctcac ctggtgaact ccggcagtcg ccgctaaatc
481 tccctaatta cacacttctt ctgccttgtg attctgcaac aagtgggtct atccctcaaa
541 atccagcccc ataaggcttc aggactgtgt ggctccagct tcagcctgca caaagtaggc
601 gcccaagcaa cactggaagc ctcggtactg aaggggcccg gaaggggcag gtgagacatt
661 ggagtcacgt ctgcaggggg ctcacctggg agcttcctag ggtgtagcca gcagggaagg
721 tctggggttc agaattccgg gaaatgcgcg ccagagttgc aggcgggggg gggggggggg
781 ggggggcggg gccgtggctc cggaaggcgg ggtctctctg tgggcgtagc gtgggcgggg
841 ctgtgcgggg cagcccggta aaaaagagcg tggcgggccg cggtctctga gagccatcgg
901 gaagcgaccc tgccagcgag ccaacgcaga cccagagagc ttcggcggag agaaccggga
961 acacgctcgg aaccatggcc cagacacccg cattcaacaa acccaaagta agcaccgagg
1021 ggctccgttg ccagggttct gtcgggctgt cccggggctt agcggggccc acctttggcg
1081 cctttaacct agaagcatgg agtggcaggg ggactcccgc aggcatctcc cctcgaccca
1141 ggccttagct tgcttccggg atgtcgagcg agagacgatg tggcagggag tgtccagaag
1201 ggctccgttg ccagggttct gtcgggctgt cccggggctt agcggggccc acctttggcg
1261 cctttaacct agaagcatgg agtggcaggg ggactcccgc aggcatctcc cctcgaccca
1321 ggccttagct tgcttccggg atgtcgagcg agagacgatg tggcagggag tgtccagaac
1381 ctgggggtgt ctctggtcgg ccttcgggtt cggctgctgt ctatgcgaac ctgggagtgc
1441 ctccagtcgg ccttcgggtt cggctgctgc ctatgccctg tgccctggag gtctcagcct
1501 cgctgtctgc caatgggcat ccagtgcggc ggggctgcac agctgtgtgg gactgggcta
1561 ggacctgggt gtctgagccc cagtagaatg gggcccaggg tctctagctg ttaaatgttc
1621 agtgtatggc tttatactta agtgttatga ttactttctg ggcaacaggt aacctaggtt
1681 tgtgggtgcg cccgtgggaa aatctatgat ccaaaccaga aaaggaaggg atagaggctt
1741 cagggtgcca ggaggaaccc ctacacatac tgaccgtttg gccatatggg tttatttggg
1801 atgaagtttt agcccattga ccccagagga gaacccttta tctgtctttc tgcaagctgt
1861 ggcttcttgg aaacagggag actccaggtc cccaaggcca gatttgcagc ccttacagat
1921 tctgtctagt cagccaggca aattgaactg gtcagcagaa gtgtgggact gagaactcag
1981 ggggagggat cagagacagt cacccttaga cttacccctc caagaaacag atgctgagtg
2041 gggggcgggg tggcagacgt atgaatcccg tgtgcatgtt gtgtcatata tgcgtgcatg
2101 gagggagcgg gagggaagat gggcagtggg cctgtattcc atgcacttac catagggaac
2161 acactctgcc cctctagcta gaggctagaa gggcagggca agtcttccta cccaaccaat
2221 gcctgctgca catcttgtct ggtggctcct gaccacagtt ggtgctctta gacatcaaag
2281 ggtgagtttt cttttgatgg tctgaatt
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM02 207 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 2 (non-coding).
ACCESSION M34243 J04767
KEYWORDS adenosine deaminase.
SEGMENT 2 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 207)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
precursor_RNA <1..>207
/note="adenosine deaminase (ADA) mRNA and introns"
intron <1..78
/note="ADA intron A"
intron 141..>207
/note="ADA intron B"
BASE COUNT 39 a 58 c 54 g 56 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 1.
1 gctcctcggg ctctgtggtg gcttctgagg tgtcctctgg ctctgtggta tctcacgctc
61 tttttctgtc ccttgcaggt agagttacac gtccacctgg atggagccat caagccagaa
121 accatcttat actttggcaa gtaagtccaa ggacaaccac agaccttccc aggattgcag
181 agcgtgtaca gctcttcttg gggggcc
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM03 382 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 3 (first expressed
exon).
ACCESSION M34244 J04767
KEYWORDS adenosine deaminase.
SEGMENT 3 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 382)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..176
/note="ADA intron B"
exon 235..299
/note="adenosine deaminase (ADA, EC 3.5.4.4), exon 3
(first expressed exon)"
intron 300..>382
/note="ADA intron C"
BASE COUNT 86 a 117 c 94 g 85 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 2.
1 aacacacaca tgcctgatgc cagcaaagga ggcctgaagg cattggtacc cctggaatta
61 gagttacagc tggtcatggg cctccatgtg ggtctcgtct tctgcaagaa cagccagtgt
121 gctcttaccc accaagccct ggtgcagccc ctcacccttg actttatttt taggaggaag
181 agaggcatcg ccctcccggc agatacagtg gaggagctgc gcaacattat cggcatggac
241 aagcccctct cgctcccagg cttcctggcc aagtttgact actacatgcc tgtgattgcg
301 taagttgctc cccaaccctt gtgccccaca gtagcatcca tccctataac caaggtcagg
361 cctgagctgc tgctgtacaa gg
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM04 346 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 4.
ACCESSION M34245 J04767
KEYWORDS adenosine deaminase.
SEGMENT 4 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 346)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..92
/note="ADA intron C"
exon 93..227
/note="adenosine deaminase (ADA), exon 4"
intron 228..>346
/note="ADA intron D"
BASE COUNT 77 a 85 c 111 g 73 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 3.
1 acagttgtag ttacctcgtt ggctactaga cgtcccaagg agctgagaaa ggttgccaac
61 ctgtgttctt cttcccttcc caggggctgc agagaggcca tcaagaggat cgcctacgag
121 tttgtggaga tgaaggcaaa ggagggcgtg gtctatgtgg aagtgcgcta tagcccacac
181 ctgctggcca attccaaggt ggacccaatg ccctggaacc agactgagtg agtgacatca
241 ctggaggggg ctgtgctgag cggggctctg agctgaggat ggagtgctta gagccctggc
301 ctggtccatg gactcagagc gactcagctc agtcctaagt gcacga
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM05 385 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 5.
ACCESSION M34246 J04767
KEYWORDS adenosine deaminase.
SEGMENT 5 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 385)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..114
/note="ADA intron D"
exon 115..230
/note="adenosine deaminase (ADA), exon 5"
intron 231..>385
/note="ADA intron E"
BASE COUNT 84 a 115 c 94 g 92 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 4.
1 tctccatcta gaaatagaag ggcagagaga catcactaca tccctgctcc agttccatgg
61 ctgcccatgg tcttcccttg gcctaaagtc ctccctcttc ctctctccac acagagggga
121 cgtcacccct gatgacgttg tggatcttgt gaaccagggc ctgcaggagg ggaggcaagc
181 atttggcatc aaggtccggt ccattctgtg ctgcatgcgc caccagccca gtgagtaccg
241 ccgcaccctg ctggctgcct ggcctataac aaggtggacc gactatccag cgtccccacc
301 tcgtatttct agagttttct aaaaaacacc tgtgaacttt tggtgactct ggtgagtcct
361 taacaggaaa ttgggacttg cacag
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM06 189 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 6.
ACCESSION M34247 J04767
KEYWORDS adenosine deaminase.
SEGMENT 6 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 189)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..17
/note="ADA intron E"
exon 18..145
/note="adenosine deaminase (ADA), exon 6"
intron 146..>189
/note="ADA intron F"
BASE COUNT 37 a 39 c 75 g 38 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 5.
1 ggcccgtgcc cctgcaggct ggtcccttga ggtgttggag ctgtgtaaga agtacaatca
61 gaagaccgtg gtggctatgg acttggctgg ggatgagacc attgaaggaa gtagcctctt
121 cccaggccac gtggaagcct atgaggtggg cctgagaagg ggagggtggc cctgggggag
181 cttgggtag
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM07 307 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exons 7 and 8.
ACCESSION M34248 J04767
KEYWORDS adenosine deaminase.
SEGMENT 7 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 307)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..10
/note="ADA intron F"
exon 11..82
/note="adenosine deaminase (ADA), exon 7"
intron 83..163
/note="ADA intron G"
exon 164..265
/note="adenosine deaminase, exon 8"
intron 266..>307
/note="ADA intron H"
BASE COUNT 68 a 86 c 85 g 68 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 6.
1 tcccttccag ggcgcagtaa agaatggcat tcatcggacc gtccacgctg gcgaggtggg
61 ctctcctgag gttgtgcgtg aggtaaggag ccagtgaccc cgggcctctt cttcctgatt
121 ctgttcctgt ccctggactc acctcctctc tgcttctcca caggctgtgg acatcctcaa
181 gacagagagg gtgggacatg gttatcacac catcgaggat gaagctctct acaacagact
241 actgaaagaa aacatgcact ttgaggtgag acgccaaggc agagagagtg agctctggct
301 accccgt
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM08 249 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 9.
ACCESSION M34249 J04767
KEYWORDS adenosine deaminase.
SEGMENT 8 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 249)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..111
/note="ADA intron H"
exon 112..176
/note="adenosine deaminase (ADA), exon 9"
intron 177..>249
/note="ADA intron I"
BASE COUNT 56 a 77 c 60 g 56 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 7.
1 ctgaggcaat gaagcacaaa gctatccaga atagaacctc agctgggctc agccctgacc
61 agtctggccc cggccactat gccagccagc cacacatcct gccccttgca ggtctgcccc
121 tggtccagct acctcacagg cgcctgggat cccaaaacga cgcatgcggt tgttcggtga
181 gatctggttc cgggacccat tttgttttga ttccggaatt cacctatagt gagtcgtata
241 aattcgtaa
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM09 340 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 10.
ACCESSION M34250 J04767
KEYWORDS adenosine deaminase.
SEGMENT 9 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 340)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..140
/note="ADA intron I"
exon 141..270
/note="adenosine deaminase (ADA), exon 10"
intron 271..>340
/note="ADA intron J"
BASE COUNT 81 a 77 c 92 g 90 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 8.
1 ttaatacgag aatgcaaccc tttgtgttgt ctaaggttgt ataaagatgg aagagggagg
61 tggtggaagg gcagtgatgg ttcttggagt gaagaggctc tctctctctc tcttttcttc
121 ctgcctggcc cctcccccag cttcaagaat gataaggcca actactcact caacacagac
181 gaccccctca tcttcaagtc caccctagac actgactacc agatgaccaa gaaagacatg
241 ggcttcactg aggaggagtt caagcgactg gtgagtatgt gtgagctatg agcctgacac
301 tggcccaggt gtgtgtgtgt gtgtatatgt gtgtgtgtgt
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM10 279 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 11.
ACCESSION M34251 J04767
KEYWORDS adenosine deaminase.
SEGMENT 10 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 279)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
intron <1..87
/note="ADA intron J"
CDS join(M34244:235..299,
M34245:93..227,M34246:115..230,M34247:18..145,
M34248:11..82,M34248:164..265,M34249:112..176,
M34250:141..270,88..171)
/note="adenosine deaminase (ADA)"
exon 88..171
/note="adenosine deaminase (ADA), exon 11"
intron 188..>279
/note="ADA intron K"
BASE COUNT 67 a 88 c 61 g 63 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 9.
1 ggatctgttt cccccactat gatgcccttg cccttgctaa cagggctgct tccttccttg
61 tcctgactcc atgtttcccc cttctagaac atcaacgcag cgaagtcaag cttcctccca
121 gaggaagaga agaaggaact tctggaacgg ctctacagag aataccaata gccaccacag
181 actgacggta cgcttgtgca gggcgcaata accaccccac cacactgtcc tccttaactc
241 tgtgcgattg tggcagaagt cttgggcagg agcacacct
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS MUSADAM11 442 bp ds-DNA ROD 12-JAN-1991
DEFINITION Mouse adenosine deaminase (ADA) gene, exon 12 (non-coding).
ACCESSION M34252 J04767
KEYWORDS adenosine deaminase.
SEGMENT 11 of 11
SOURCE Mouse lung fibroblast cell line B-1/200 DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 442)
AUTHORS Al-Ubaidi,M.R., Ramamurthy,V., Maa,M.-., Ingolia,D.E.,
Chinsky,J.M., Martin,B.D. and Kellems,R.E.
TITLE Structural and functional analysis of the murine adenosine
deaminase gene
JOURNAL Genomics 7, 476-485 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
precursor_RNA <1..>287
/note="adenosine deaminase (ADA) mRNA and introns"
intron <1..69
/note="ADA intron K"
misc_signal 282..287
/note="poly-A signal"
BASE COUNT 97 a 111 c 114 g 120 t
ORIGIN Chromosome 2; undetermined number of base pairs after segment 10.
1 ttctgtgctt ctaccatgcc ttacatgtca tgagacctga cctttctatt tctctgactt
61 gaccagcagg gcgggtcccc tgaagatggc aaggccactt ctctgagcct catcctgtgg
121 ataaagtctt tacaactctg acatattgac cttcattcct tccagacctt ggagaggcca
181 ggtctgtcct ctgattggat atcctggcta ggtcccaggg gacttgacaa tcatgcacat
241 gaattgaaaa ccttccttct aaagctaaaa ttatggtgtt caataaagca gctggtgact
301 ggtatcttgc agcacatggt gaatacggtc tcggggctgc tggctaggat gctaagaaag
361 gaggagcctg ggccctacgc tgagtgtcag gtctggggag ctagggtctc ttccgcaggt
421 cgactctaga gatccccggg ct
//