GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS ECO67RTDM 11334 bp ds-DNA BCT 12-JAN-1991
DEFINITION E.coli retron Ec67 DNA encodong reverse transcriptase and Dam
methylase functions.
ACCESSION M55249
KEYWORDS Dam methylase; reverse transcriptase.
SOURCE E.coli retron Ec67 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 11334)
AUTHORS Hsu,M.-Y., Inouye,M. and Inouye,S.
TITLE Retron for the 67-base multicopy single-stranded DNA from
Escherichia coli: A potential transposable element encoding both
reverse transcriptase and Dam methylase functions
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9454-9458 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS complement(29..625)
/note="ORFi"
/codon_start=29
CDS 616..939
/note="ORFa"
/codon_start=616
CDS complement(796..1110)
/note="ORFh"
/codon_start=796
CDS 975..1484
/note="ORFb"
/codon_start=975
CDS 1656..1997
/note="ORFc"
/codon_start=1656
CDS 2522..2824
/note="ORFd"
/codon_start=2522
CDS 2821..3678
/note="ORF1-dam"
/codon_start=2821
/product="Dam methylase"
CDS 3164..3496
/note="ORFj"
/codon_start=3164
CDS 3734..5137
/note="ORF2"
/codon_start=3734
CDS 5017..5628
/note="ORF3"
/codon_start=5017
CDS 5625..6089
/note="ORFe"
/codon_start=5625
CDS 6369..6674
/note="ORFf"
/codon_start=6369
CDS complement(6886..7203)
/note="ORFg"
/codon_start=6886
CDS 7839..9599
/note="ORF4-Ec67 RT"
/codon_start=7839
CDS complement(9687..10667)
/note="ORF5"
/codon_start=9687
BASE COUNT 3091 a 2463 c 2774 g 3006 t
ORIGIN
1 gaattcattt ttaaatcctt ttactatatc aatttgaaac tattgtactt cttatacaag
61 caaccacttc aatatcagaa aatgcacatt caaagctatt ctctgtagtt gtaattttaa
121 tcataccttt tggaagcttt gtgatttttc gaatggaata ggttccgtcg atgttgatta
181 accagtgtcc atcaagtact tcagaaaatt tttgatcaca aatgtatgtg acaagcccat
241 cctgaataac aattggtgat gaaagatttg caggaagaaa tgatgaatcg aatacatatg
301 aaccatcttc aatcattttg cctgcggtaa ggcgaaactt gggaagctct tctgtggcta
361 ctgtcagcga gctttgctta agtccttgcc cggtggttaa ccatttcagt gaggttcctg
421 tttcaagagc acattggatt atccagtctg caggaaacgt atcccgcaaa tacctgttcg
481 ccagagtgct ttttgatact tccaagtgat cagctaaagc ctgtcgtgtc gtaaagccat
541 aagcttcgac taatcgctca atcgcagcct taccaccttt attgggattt attttgatct
601 cacttgggta ctttgatgtt gacatatctc ttttgcgatc ctagtatcag ttttgtcccc
661 atttgggtac ttgtcacgat tactacaggc tcaccacaag ccaataggag atgttgcatc
721 atgaccccta acatttcaat aactctgaat acgccacatg tcacaattga gcgttatagc
781 gaacttactg gtctttcaat cgacacaatt aacgatatgc tggctgacgg tcgcatccct
841 cggcatcgcc ttcggaaaga caagaaaaga gaaaaggtga tgatcaacct tgctgctctt
901 accgttgatg cacttactga ttgcaatgtt gtattcaact agttccattt tgggatacat
961 caggggtgtc gaccatgttt gattaccaag tttccaaaca tccacatttt gatgaagcct
1021 gtcgtgcatt cgcattgcgc cacaacctgg tgcaactggc agaacgtgct ggcatgaatg
1081 tgcagattct gcggaacaag ctgaacccat ctcaacctca tttattaacc gcaccagaaa
1141 tctggctgct taccgatctg actgaagatt caacgctggt agatggtttt ctggcacaga
1201 ttcactgcct gccatgtgta ccgattaatg aggttgcaaa agagaaactg ccgcattacg
1261 tcatgagcgc aactgcagag atcggacgtg ttgcagcagg tgcggtatct ggtgatgtaa
1321 aaactagtgc cggtcgtcgt gatgctatca gcagcattaa ctctgtaaca cgactgatgg
1381 cgctggctgc tgtttcattg caggcccgtt tacaggctaa tcctgcgatg gcgagtgcag
1441 ttgataccgt gactggcctc ggtgcttcat tcggtttgct gtgaggtgct tatgctgacg
1501 aaagaaccat catttgcatc gctgctggta aaacaaagcc cggcaatgca ctacggtcac
1561 ggctggatca tgggtgagga tggtaaacgc tggcatccgt gccgttcaca agatgaattg
1621 ctggcagaac tatctacgaa aaaacggggg aacaaatggc tattgaaggc gctgcggcga
1681 ctgttccatt aagccccggt gaacgcctga atggacttaa tcacattgcg gaattaaggg
1741 cgaaagtatt tggcctgaat attgagtcag agcttgagcg gtttattaaa gatatgcgtg
1801 atccacggga tatcaataat gaacaaaata aacgagcact ggctgccata ttctttatgg
1861 caaaaattcc agctgaacgt catagcatca gcattaatga gctgaccact gacgaaaagc
1921 gggagttgat taaagcaatg aatcattttc gtgcagtggt gagcttattt cccagacggc
1981 taaccatgcc gaattaacca actaatgaaa ttaatggcgt aaacccgccg ggtatccctt
2041 tatctaaatt caggagaatt gattatgcgt aatattgaaa ctctcacgac taaaaccgga
2101 ccggatgatg cagggcttaa tattttactg acagaggctc gtctggaaga acgccgggca
2161 agggctgaag caatggcagc tcgccttgat agcctggcgt gtcatatcac atcccgccag
2221 ctaaaccacg tcgaagcagc agaactgctg cgtgtaactg ctgaagcaat ccagaacgaa
2281 gcgcaggaga tccactaatg gctgatgcaa tggatctcgt acagcagcgc gttgaagaag
2341 aacgccagcg ccatatccgt gctgcccgtg ccaaaacgcc gggcgtgtcc cgcgtgcttt
2401 gcattgagtg tgaagcgcca attccgccag cacgccgccg tgccattccg ggtgtgcagc
2461 tttgcattac ctgtcaggaa atcgcagagc tgaaaggcaa acattacaac ggaggtgctg
2521 tatgacaagg gcagtgcgta tccatcaatt aaaaattgca cctaagtatt tcaacgctgt
2581 ggttgcaggt caaaagacgg ctgaacttcg taaagacgat cgtggctata aagttggtga
2641 tgttctttct ctttgcgaat ggaagcatgg cgtatttacg ggtagggaat gggccgctgt
2701 tatctctcat gtgcttccgg ttaatgacgt catggcagtt tcagaacaat gggtgatgct
2761 atcaattcgc ccattaaccc cattagaagc tttaggatat gttattgcag gaggtgctgt
2821 atgagcacca tcctgaaatg ggcgggaaat aaaaccgcca ttatgtccga actgaaaaaa
2881 caccttcctg ctggcccgcg actggttgaa cctttcgcgg gttcctgtgc tgtgatgatg
2941 gcgacggatt accccagcta tctggttgcg gatattaatc ctgatttaat caacctctat
3001 aaaaagattg ccgctgattg cgaggcgttt atatctcgcg ccagagtttt atttgagatc
3061 gcaaacaggg aggtggctta ttacaacata aggcaggagt ttaattactc aactgaaatt
3121 actgatttca tgaaagcggt atatttcctg tatctcaatc gtcatggtta ccgtgggtta
3181 tgtcgctata acaagagcgg gcatttcaac attccctacg ggaattataa aaatccgtat
3241 ttccctgaaa aagaaattcg caaatttgca gaaaaagccc agcgagcaac gtttatctgc
3301 gccagctttg atgaaacgct ggcgatgctg caggtggggg atgtggtgta ttgcgatccg
3361 ccttatgacg gtacgttttc cggctatcac actgacggct tcactgaaga tgaccagtat
3421 cacctggcat ctgttcttga atatcgatca tcagaaggtc atccagtcat tgtttctaac
3481 agtgacacat ccctgatccg ttcgctgtat cgcaatttca ctcaccacta catcaaggca
3541 aaacgcagca tcggcgtgtc ggctggcgag agtaaatctg caacagaaat cattgctgtt
3601 tccggggcgc gctgctgggt gggatttgat ccttcgcgtg gcgtggatag ttctgctgtg
3661 tacgaggtgc gtgtatgagt catgccgata tgagcgactc tagcggcttt aacgaggccg
3721 ctgcagcatt ttcatggaac ggcccgaaaa aggccataaa cccttatctg gacccggcgg
3781 aagttgcgcc gtttctgcgc tttcaaacct gatcactctg tatgctgccg ataacgagca
3841 ggaacagctg cgccgcgagg aattgagtga acaggtctgg gagcgttatt tctttaatga
3901 atcccgtgat cctgtccagc gcgaaatgga gcaggataag ctcattagtc gggcaaagct
3961 ggcgcatgag cagcagcgtt ttaacccgga catggtcatt ctggcggacg tcagcgccca
4021 gcccacccat atcagcaagc cgctgatgca acgtatcgaa tacttcagca gcctgggcag
4081 gccaaaggct tattcccgct atttgcgtga gacgattaag ccatgtctgg agcgactgga
4141 ttgtgtacgt gacagtcagc tatctgcttc tttccgtttt atggcaagcc atcaagggct
4201 ggagggcctg ctgatcctgc ctgaaatgag tcaggatcag gtgaaacgcc tgtccaccct
4261 ggtagcagcg catatgagca tgtgtcttga ggccgcttgt ggtgatttgt atgccaccga
4321 tgacgttaag ccagaagaaa tccgcaagac atgggaaaag gtggcagcgg aaaccctgcg
4381 tctggatgtt atcccgcctg cgtttgagaa acttcgtcgg aaaagaaacc gccgcaaacc
4441 cgtgccctat gaactcatac cgggttcgct ggcacgtatg ctttgcgccg actggtggta
4501 tcggaaattg tggaagatgc gttgcgaatg gcgggaagag cagttgcgtg ctgtctgtct
4561 ggtcagcaaa aaagcatctc cctatgtcag ctatgaagca gtgacacata aacgtgagca
4621 gcgccgcaag tcgctggagt ttttccgttc tcatgaactg gtgaatgaag acggagacac
4681 gctggatatg gaggatgtgg taaacgccag cagcagcaat ccggcgcatc gccgcaatga
4741 gatgatggcc tgtgttaaag gtctggagct tatcgcagaa atgcgcggtg actgcgccgt
4801 tttctacact atcacctgtc cgtcacgttt ccattccacg ctcaacaacg gcagacccaa
4861 cccgacctgg acaaatgcga cggtaagaca aagcagcgat tatctggtcg gcatgtttgc
4921 tgcatttcgt aaggctatgc aaccaaagcc gggttgcgct ggtatggcgt gcgggtggct
4981 gagccgcacc atgacggtac tgtgcactgg catctcatgt gtttcatgcg caaaaaagac
5041 cgccgcgcca ttactgcatt gttgcgtaag tttgccatcc gtgaagaccg tgaggaactg
5101 ggcaataaca cggggccacg ctttaagtct gagctgataa acccgcgcaa aggaacgccg
5161 acaagctaca tcgcgaaata catcagtaag aacattgacg gacgtggtct ggctggcgag
5221 atcagcaagg aaacgggtaa atccctgcgt gataacgccg aatacgtgaa tgcctgggcg
5281 tctctacatc gtgttcagca gttccgcttc tttggtattc cggggcgtca ggcgtaccgt
5341 gaacttcgct tgctggctgg tcaggcggca aggcaacagg gtgacaaaaa agcaagtacg
5401 ccgatactgg atgacccgcg ccttgatgcc atcctggctg ccgctgatgc tggctgtttt
5461 gccacctaca tcatgaagca gggcgcgtac tggttccccg caaatatcac cttatcagaa
5521 cagcttatga aattaacgaa gagccgaccg cttatggcga tcacggtatt cgtatttatg
5581 gcatctggtc acccattgta cagggcaaga tctgcactca tgcaatgaaa tggaaaatgg
5641 ttcgtaaggc cgttgacgtt caggaggcgg cagccgacca gggcgcttgc gccccttgga
5701 ctcgtggcaa taactgtccc cttgctgaaa atttgaacca acaggagaaa gataaatcag
5761 ctgatgggga caccagaacg gacattaccc gcatggatga caaggagttg cacgatcacc
5821 tgcacagtat gagcaaaaaa gagcgccggg aactggctgc aaggttacgc ctggttaaac
5881 cgatacggcg taaagactac aaacagcgaa ttacagatca tcagcgacag cagctcgttt
5941 atgagctgaa gtccagagga tttgatggca gcgagaaaga ggtcgattta ctccttcgcg
6001 gcggcagtat tccgtcagga gcaggcctgc gtatcttcta tcggaaccag cgtctgcagg
6061 aggatgataa atggcggaac ctgtattaat tacgctggtt aacaattcgt gctcttaata
6121 ataccaggca tatcaggctg atgagcgtaa aaaaacgttt tacatcagta agattattat
6181 atactgtaaa tataaacagt ggttatgtgt acagtattgc ttgtggtgtc ataggaggaa
6241 aaatgcagga ctattttttg gagtctttga agctccagcg cattgatttt tttcttaagc
6301 ttgtagcggc tagtgagtgt agtgatgaag agaaggggct ggcctgcagt gggtttctga
6361 actgacagat gaactcatgg caaaaatcag aacccacgaa tacaaccgct caatggatgt
6421 catcagttga ggtgactttt atgcgcattg aaataatgat cgataaagag cagaagatta
6481 gccagtctac cctggacgcc cttgaatccg agctttaccg caatctgcgc cccctgtatc
6541 ccaaaacggt aattcgtatc cgtaaaggta gctctaacgg tgtggaactg accggactgc
6601 aactggatga agaaagaaaa caagtgatga aaattatgca gaaggtgtgg gaagacgaca
6661 gctggctgca ttaagaaacg ttgcccccag gaggattcat tctgatgggg gctagtttgg
6721 gcaacgagtg aaacgaggcg taaggtgggt gggcattttg ataagtgatc gtccgctttg
6781 tgtcagaagc agaagtggga gtgtctgaga ctctctaaaa gttgatgatt cacttacaaa
6841 accattttcc tgatgaatgc ttacacttac gaatgatcat tctgtttagt cttcatgaga
6901 aaatcccgaa tctttgccaa gtttgaattt tcggggattg aatccatgta cggattcacg
6961 ctcaaacact aattgctcag attcaacgaa agcctgccac tctactgggt ctagctcaaa
7021 tcccattgct gtcatatcac tttcatcttc cccctcgatc cagtggacta aataaaacaa
7081 ttcatcatca ggagaatcat cctcaccatc aacaatatca acatcggtta cagtgatacg
7141 tagggtggga tcgatttttg agacataaat tcctaactgt ggtatagggg gaatattgtt
7201 catggaaact ccttttattg gtgtttgcta tttgaataaa aatacgccaa acagagtgtt
7261 aactgtgaat atggaaagaa tcgttgtaag tacccgctta ggtgtcccat tctattttag
7321 aggttctcgt gcatatggat tatgttatct gaaagctaac ttccgcttcc cgttcacagc
7381 gaacattcat ctttgtaaac ccgtatgatc cgtttttcaa gtggccattc agatacggat
7441 tttcacttcc ttgacagtgc atgactatgc tgcatgaaat cgcatgatcg attgaggatc
7501 gtctttgctc agatccgcca gaactggcgg gcttttgctc atgtcatgca tgtgcatgaa
7561 aaccactgca taaagcgggc aggcgtggcg gggatacgag cgcgcgctat caccgaaaat
7621 agccaaaata cttctggaaa acagaaagtt gaagtgatat gttcataaac acgcatgtag
7681 gcagatttgt tggttgtgaa tcgcaaccag tggccttaat ggcaggagga atcgcctccc
7741 taaaatcctt gattcagagc tatacggcag gtgtgctgtg cgaaggagtg cctgcatgcg
7801 tttctccttg gccttttttc ctctgggatg aagaagaaat gacaaaaaca tctaaacttg
7861 acgcacttag ggctgctact tcacgtgaag acttggctaa aattttagat attaagttgg
7921 tatttttaac taacgttcta tatagaatcg gctcggataa tcaatacact caatttacaa
7981 taccgaagaa aggaaaaggg gtaaggacta tttctgcacc tacagaccgg ttgaaggaca
8041 tccaacgaag aatatgtgac ttactttctg attgtagaga tgagatcttt gctataagga
8101 aaattagtaa caactattcc tttggttttg agaggggaaa atcaataatc ctaaatgctt
8161 ataagcatag aggcaaacaa ataatattaa atatagatct taaggatttt tttgaaagct
8221 ttaattttgg acgagttaga ggatattttc tttccaatca ggatttttta ttaaatcctg
8281 tggtggcaac gacacttgca aaagctgcat gctataatgg aaccctcccc caaggaagtc
8341 catgttctcc tattatctca aatctaattt gcaatattat ggatatgaga ttagctaagc
8401 tggctaaaaa atatggatgt acttatagca gatatgctga tgatataaca atttctacaa
8461 ataaaaatac atttccgtta gaaatggcta ctgtgcaacc tgaaggggtt gttttgggaa
8521 aagttttggt aaaagaaata gaaaactctg gattcgaaat aaatgattca aagactaggc
8581 ttacgtataa gacatcaagg caagaagtaa cgggacttac agttaacaga atcgttaata
8641 ttgatagatg ttattataaa aaaactcggg cgttggcaca tgctttgtat cgtacaggtg
8701 aatataaagt gccagatgaa aatggtgttt tagtttcagg aggtctggat aaacttgagg
8761 ggatgtttgg ttttattgat caagttgata agtttaacaa tataaagaaa aaactgaaca
8821 agcaacctga tagatatgta ttgactaatg cgactttgca tggttttaaa ttaaagttga
8881 atgcgcgaga aaaagcatat agtaaattta tttactataa attttttcat ggcaacacct
8941 gtcctacgat aattacagaa gggaagactg atcggatata tttgaaggct gctttgcatt
9001 ctttggagac atcatatcct gagttgttta gagaaaaaac agatagtaaa aagaaagaaa
9061 taaatcttaa tatatttaaa tctaatgaaa agaccaaata ttttttagat ctttctgggg
9121 gaactgcaga tctgaaaaaa tttgtagagc gttataaaaa taattatgct tcttattatg
9181 gttctgttcc aaaacagcca gtgattatgg ttcttgataa tgatacaggt ccaagcgatt
9241 tacttaattt tctgcgcaat aaagttaaaa gctgcccaga cgatgtaact gaaatgagaa
9301 agatgaaata tattcatgtt ttctataatt tatatatagt tctcacacca ttgagtcctt
9361 ccggcgaaca aacttcaatg gaggatcttt tccctaaaga tattttagat atcaagattg
9421 atggtaagaa attcaacaaa aataatgatg gagactcaaa aacggaatat gggaagcata
9481 ttttttccat gagggttgtt agagataaaa agcggaaaat agattttaag gcattttgtt
9541 gtatttttga tgctataaaa gatataaagg aacattataa attaatgtta aatagctaat
9601 gaacagccct aacgttatga acgctaaggc tgatttttcg ttaaaattta tatggtttga
9661 attgtaatat attatcttca agccatttat ttaattcctg catccttttc tgtaagggta
9721 ttaattcgtt cctcacaaac actaaactcg ctttttccac atccccaaac ccccctaaca
9781 ttattcggca taatccccat catttgcggt ggcacacgat gcgctgccat catgtcatcg
9841 cggctgacat ttttgatatt cagaaactca tccttcgccg cgacttctga caatgggatg
9901 atctgaagtc cgtccttttt gccgttaggc gagtacataa acagattgcg gaagttacct
9961 ggtcctttgg cgcttttcat cgcattgcgg aggttgttca catcctcctg gttctgcgca
10021 gcatcggtca tgtacatgat gaagcctgca tgactgccgt taatgtaata cttgcggcgg
10081 aacagcgtgg cggactcgtt gagcagagct gacggaatgg cagaaagata accgggcagg
10141 ccgtagatct cctggttgat gtccggttcc aacagatgaa agatgctgcc tttcgtgaac
10201 tgatacggct gggttgtcat accgtattgc acaaaccagt aggtatccag gtctaacccg
10261 cgtcgggtgt attttgccag cgcaggctca agagcgatga cctctccgaa gcggttcgtg
10321 cgtttttcca ggtaggcgtt accaaaaacc agatagtcct gtacaaaacg cgaaaaagcc
10381 tgctggctga gcagcgggtg agggatgtag gtgctggtca gaatgttgca tttcaccgca
10441 attggtgaac tgtgatgcac ggcagcgcgg aaggtgcgcg ccagtccgtc aaagctgaca
10501 ggaggctcat accagcgatc tgtctgtacg cattccacat agtccagcag ttcgcggcgg
10561 tcaagtacag gaacgggatc accgaagctg aatgcttcgg ctgaagtctg gcttttatgc
10621 ttgagctgat tcgtcgccgc agcgcggttt ttcttactct ttcccatcaa aaaatctcca
10681 caatattgct ggtattggcg gattcgccct gcagcggttc gttaaacaat gcgtgcatcg
10741 ttgcccaggc cagatcggcg tggctggctt cttcgctgcg gctggcttca taggtcgggc
10801 ggttgccgct ggcggtggtg gcgcgacgga ttgccatgaa ggactgcgca atgtcggtgt
10861 gtccggcgtc aaattctagg cggcggtgac tgataatgtc gtaggccttg agtaccaggg
10921 cgtttttaac gttggggttg tagacaaact cccggacggc aggaaagaat gctttcacgt
10981 tctcgtaaac cccgtggccg acgccagttg agtcgatgcc gatgtatgtc acgttgtact
11041 gttcggtcag ttttttgatg gcgtcagcct ggcgcggaag tccatcccgc gccactggtg
11101 acgctcaaga atgcggaact taccgcccgg cacggctggc ggtgccacca ccacgcaccc
11161 ggccgtgtcg ccgttttgcg taccttttgc cgggtcataa ccgatccaca cttcgcgcca
11221 gccaaacggg cgcagggcca gtgcatgaaa gtcggtccag acttcccagc tgtccaccat
11281 gcacgcctgc agctcgctga gcgggaacac ggacgcgaga tcgtccacga attc
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