GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS TRBGRNA 276 bp ds-DNA INV 12-JAN-1991
DEFINITION T.brucei minicircle guide RNA (gRNA).
ACCESSION M55992
KEYWORDS guide RNA.
SOURCE T.brucei minicircle DNA.
ORGANISM Trypanosoma brucei
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE 1 (bases 1 to 276)
AUTHORS Polland,V.W., Rohrer,S.P., Michelotti,E.F., Hancock,K. and
Hajduk,S.L.
TITLE Organization of minicircle genes for guide RNAs in Trypanosoma
brucei
JOURNAL Cell 63, 783-790 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
misc_feature 6..17
/note="universal 12 mer minicircle sequence"
repeat_unit 139..156
/rpt_type=18 bp inverted repeat
misc_feature 191..232
/note="region complementary to COIII edited mRNA"
repeat_unit 259..276
/rpt_type=18 bp inverted repeat
BASE COUNT 93 a 22 c 52 g 109 t
ORIGIN
1 cgaaaggggt tggtgtaata cacacactgc tttttctgag tttttcaggg aaattcgggt
61 attttcatag gtttggtgga tattgttgtt ggaattataa gagttaaata tctcagttat
121 atcggtgtct aagataaaga aatgaggtaa tagatatagt tatgtagtat tataattaga
181 tattgtatat ataaaacaaa ctcgctatgt aagaactgta aaaagtgata ttatttactg
241 tatatgatat taatattcta tttattatct tattta
//