GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS STASAU3AIM 3360 bp ds-DNA BCT 12-JAN-1991
DEFINITION S.aureus Sau3AI-restriction-enzyme and Sau3AI-modification-enzyme
genes, complete cds.
ACCESSION M32470
KEYWORDS Sau3AI-modification-enzyme; Sau3AI-restriction-enzyme.
SOURCE S.aureus (strain 3AI) DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 3360)
AUTHORS Seeber,S., Kessler,C. and Goetz,F.
TITLE Cloning, expression and characterization of the Sau3AI restriction
and modification genes in Staphylococcus carnosus TM300
JOURNAL Gene 94, 37-43 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence [Unpublished (1990)
Univ. of Tuebingen, D-7400 Tuebingen 1] kindly submitted by
S.t Seeber, 02-MAR-1990.
FEATURES Location/Qualifiers
misc_binding 437..441
/note="ribosome binding site"
CDS 448..1917
/note="Sau3AIR protein (ttg start codon)"
misc_binding 2004..2010
/note="ribosome binding site"
CDS 2017..3255
/note="Sau3AIM protein"
BASE COUNT 1295 a 393 c 638 g 1034 t
ORIGIN
1 aagtcgacct tcaccaagac cgaattttcc gcgtataccc gcagtaccaa acgttaattt
61 actttcaaaa ccttctcgct gttcaatgtc agattgctgc tcataaaaat gtttaactaa
121 actatcatta gctctttcta tccataattc tttatccatt gttgctaaac atcctttcaa
181 aatctcagtt agacttaata aaacatgaaa actaaagccc ttacatttat gtaatgaatt
241 ataaagaaat acgccccaaa agtaaaaaaa cacagcccca agacaatact tttcacaagt
301 attatataat agatgtgtat gaaaatgcat ggagtagatg taagagtgat attcaaaatg
361 tgtaaaaaat atggataatt ctatataatt atattattga aattttaaat agcggaaata
421 attatttaat gttaagaggg gataattttg gaaagttatt tgacaaaaca agccgtacat
481 aatcgcgcaa aagaagctgt tggtaaaagt gtattagaat taaatggtgg tgaatcgatt
541 aaacaaagta agagttcagt tggtgatgca tttgaaaatt ggtttggtaa gaaaaaagac
601 agtgatagta aaccagatat ggcagaagct ggggtggaac ttaaggcaac gccatttaaa
661 aagttgaaaa acggaaagta tagctccaaa gaaagattag tattaaatat tataaactat
721 gagaaagtgg caaatgaaaa ttttgaaact agtagttttt tatctaagaa taatactata
781 gaattagctt tctatgaata tatcaaggga acacctagtg ataattggat tattaaagaa
841 gcggtgcttt atgaaatgca taaaaacccg attgattatg aaataattaa acaagattgg
901 gaaataataa atcaatatat taatgaagga aaggcacatg aattgagtga aggtttgaca
961 agttatttag cgccatgtac aaagggtgcg aatgctagtt ctttaagaaa tcagccttat
1021 tcagacataa aagcaaagca aagagcattt tctttgaagt ctgggtatat gacatctatt
1081 ttacgcaaat atgttctagg tgatgaaaaa atagattcaa ttgtcaaaga cccatttgaa
1141 ataaaagaaa aatcaataga ggacatagtc tttgaaaaat ttcagccata tataaactgg
1201 tcaatcgata aattatgcga acatttttct atcaataaag gtgagaaagg tttaaattat
1261 agaatagcct ctgccatttt aaatctaaaa ggtaaaacta ctaaaagtaa accattcccg
1321 gaagttgaag agtttgaaaa atcatctata gtagtcaaaa cagttcattt taataaaaag
1381 aatgtgaata aagaaagtat gtcatttgga gcttttaaat ttgaagaact agctaatgag
1441 gaatgggaag atagtgaagg atatcctagt gcacaatggc gaaacttttt gttagaaaca
1501 aggtttttat tttttgttgt taaagaagat gaagatggtg tagacatatt caaaggaata
1561 aaatttttta gtatgcctga agaagacata aacggacctg tcaaaagaat gtgggatgat
1621 acagtgaaga aattaaaaga gggtgtcaca ttagaagctg taccggacaa aagtacaaag
1681 gatggttgga gaataaaaaa taattttgta gataaaagtg atgatttaat ttgccatgtt
1741 agaccacaca ctaataacag agactatcgt ggaggaagta atgcagataa gcttcctaaa
1801 aagattaact ggattaatag acctgactca gatgattatt cggatgagtg gatgactaaa
1861 caaagttttt ggataaataa tgactacata aaaaagcaag ttgaagattt attgtagtta
1921 aagtatgtta aaatataaga tattctttta aaatatcgaa cgatcgttcg tattttgtgt
1981 tataataagg ttgaattaag tataggaggt cgcctaatga ataaaattaa agtagtagaa
2041 ttgtttgcgg gtgtaggcgg gtttcgttta ggtttagaaa atacgaaaaa tggtatattt
2101 gacataactt gggcaaatca atgggagccc tcacgaaaaa tccaacatgc atttgattgt
2161 tatagtaaaa gatttaagaa cggcatccat agtaataagg atattgccca ggtatctgat
2221 gaagaaatgg caaatactga agctgatatg attgtaggag gatttccttg ccaagattat
2281 tcagttgcaa ggagtttaaa tggagaatta ggaatacaag gaaaaaaggg cgttttattc
2341 tggcaaatta ttagatatat tcaaaataca tttcctaaat acttgttgct tgaaaatgtt
2401 gatagattat tgaaatcacc ttcgagtcag agagggagag actttgctgt aatgttatca
2461 accttaaatg agttaggcta taatgttgaa tggcgcgtga ttaatgctgc tgattatggc
2521 aatgctcaaa gacgtagaag ggtatttata tttggatata agcaagattt aaactatagc
2581 aaagctatgg aagaaagtcc gttggataaa attatatatc acaatggttt gtttgctgaa
2641 gcttttccga ttgaagatta tgccaataaa aatagagtaa ataggactca tattactcat
2701 gatatagtcg atatttcaga taatttcagt tttcaatttt ataatagtgg aatcatgaaa
2761 aatggagaaa ttttaactat tgacacaata ccaaaatatg aaaaatcagt aaccttagga
2821 gaaattattg aaagtaatgt agatgatggt ttttcattaa atcaagatca aattgataaa
2881 tttaaatatt taagaggacc caaaaagatt aaacgaacta ctaaagatgg tcatgaatat
2941 tatttttcag aaggtggtat gtctgaaaca gattcattag agttacctgc aagaacaatg
3001 cttacaagtg aatcatctat taatagaagt actcattttt taaacgtaga tggtgtttat
3061 agaactttga cacctattga agcagaaagg ttgaatgggt ttccagataa ttggacagaa
3121 ggtatgccaa ttaagatgag atacttttgt atgggcaatg ctcttgttgt gcctttgatt
3181 actagaatag gtaatcaaat tgaaaaaatt gatagtatta caaatgatga attcagtcag
3241 ctacgtttat tttaaataaa tcaaatgtag agtgcgtttg atttaacaag tttgtatact
3301 aaagattcat agattattgt atattgacca gtatttatca gcgtattatt ttaatatata
//