GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS ECOABC 1993 bp ds-DNA SYN 12-JAN-1991 DEFINITION Synthetic plasmid (for E.coli) DNA. ACCESSION M34519 KEYWORDS b-galactosidase; b-lactamase; bla gene; lacZ gene; promoter. SOURCE Synthetic DNA. ORGANISM Cloning vector Artificial sequences; Cloning vehicles. REFERENCE 1 (bases 1 to 1993) AUTHORS Mandecki,W., Hayden,M.A., Shallcross,M.A. and Stotland,E. TITLE A totally synthetic plasmid for general cloning, gene expression and mutagenesis in Escherichia coli JOURNAL Gene 94, 103-107 (1990) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)] kindly submitted by W.Mandecki, 22-MAY-1990. Author address:W.Mandecki Abbott Laboratories Corporate Molecular Biology D93D Abbott Park, IL 60064 FEATURES Location/Qualifiers misc_feature 81..327 /note="lacZ fragment mRNA" CDS 119..301 /note="b-galactosidase" misc_feature 136..137 /note="SmaI site for introduction of multicloning" misc_signal 301..336 /note="trpA terminator" misc_signal 337..402 /note="bla promoter P3" mRNA 403..1329 /note="bla mRNA" CDS 438..1298 /note="b-lactamase" misc_signal 1299..1342 /note="phage fd terminator" misc_signal 1343..1408 /note="RNAII promoter" mRNA 1409..1962 /note="RNAII mRNA" misc_signal 1415..1440 /note="RNAI terminator" mRNA 1422..1517 /note="RNAI mRNA" misc_signal 1518..1555 /note="RNAI promoter" misc_feature 1961..1962 /note="RNaseH cleavage site" BASE COUNT 524 a 475 c 500 g 494 t ORIGIN 1 gaattgatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctg 61 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 121 gactatgatt acgcccgggc ttgccgtcgt tttacagcga cgagactggg aaaatcctgg 181 cgttacccaa cttaatcgcc ttgccgcaca cccccctttc gccagttggc gtaatagcga 241 agaagcccgc accgaccgcc cttcccaaca gttgcgtagt ctgaatggcg aatggcgtta 301 aactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct 361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag 541 aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc 601 ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat 661 gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact 721 attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa 781 tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact 841 tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag 901 accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg 961 agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg 1021 aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg 1081 caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg 1141 caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc 1201 gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga 1261 ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct 1321 tttggagcct ttttttttgg acggaccgag tagaaaagat caaaggatct tcttgagatc 1381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 1441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 1501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 1561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 1621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 1681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 1741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggacaaagg 1801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 1861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 1921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 1981 ttttacggtt cct //