GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS ECOABC 1993 bp ds-DNA SYN 12-JAN-1991
DEFINITION Synthetic plasmid (for E.coli) DNA.
ACCESSION M34519
KEYWORDS b-galactosidase; b-lactamase; bla gene; lacZ gene; promoter.
SOURCE Synthetic DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 1993)
AUTHORS Mandecki,W., Hayden,M.A., Shallcross,M.A. and Stotland,E.
TITLE A totally synthetic plasmid for general cloning, gene expression
and mutagenesis in Escherichia coli
JOURNAL Gene 94, 103-107 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by W.Mandecki, 22-MAY-1990.
Author address:W.Mandecki
Abbott Laboratories
Corporate Molecular Biology D93D
Abbott Park, IL 60064
FEATURES Location/Qualifiers
misc_feature 81..327
/note="lacZ fragment mRNA"
CDS 119..301
/note="b-galactosidase"
misc_feature 136..137
/note="SmaI site for introduction of multicloning"
misc_signal 301..336
/note="trpA terminator"
misc_signal 337..402
/note="bla promoter P3"
mRNA 403..1329
/note="bla mRNA"
CDS 438..1298
/note="b-lactamase"
misc_signal 1299..1342
/note="phage fd terminator"
misc_signal 1343..1408
/note="RNAII promoter"
mRNA 1409..1962
/note="RNAII mRNA"
misc_signal 1415..1440
/note="RNAI terminator"
mRNA 1422..1517
/note="RNAI mRNA"
misc_signal 1518..1555
/note="RNAI promoter"
misc_feature 1961..1962
/note="RNaseH cleavage site"
BASE COUNT 524 a 475 c 500 g 494 t
ORIGIN
1 gaattgatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctg
61 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
121 gactatgatt acgcccgggc ttgccgtcgt tttacagcga cgagactggg aaaatcctgg
181 cgttacccaa cttaatcgcc ttgccgcaca cccccctttc gccagttggc gtaatagcga
241 agaagcccgc accgaccgcc cttcccaaca gttgcgtagt ctgaatggcg aatggcgtta
301 aactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct
361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag
541 aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc
601 ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat
661 gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact
721 attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa
781 tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact
841 tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag
901 accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg
961 agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg
1021 aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg
1081 caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg
1141 caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc
1201 gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga
1261 ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct
1321 tttggagcct ttttttttgg acggaccgag tagaaaagat caaaggatct tcttgagatc
1381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
1441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
1501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
1561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
1621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
1681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
1741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggacaaagg
1801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
1861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
1921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
1981 ttttacggtt cct
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