GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS DVUHYDAB 3180 bp ds-DNA BCT 12-JAN-1991
DEFINITION D.fructosovorans periplasmic hydrogenase (hydA and hydB) gene,
complete cds.
ACCESSION M35333
KEYWORDS periplasmic hydrogenase.
SOURCE D.fructosovarans (strain DSM 3604) DNA, clone DHS-alpha-[pHH7].
ORGANISM Desulfovibrio fructosovorans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Sulfate- or
sulfur-reducing dissimilatory bacteria.
REFERENCE 1 (bases 1 to 3180)
AUTHORS Rousset,M., Dermoun,Z., Hatchikian,C.E. and Belaich,J.-P.
TITLE Cloning and sequencing of the locus encoding the large and small
subunit genes of the periplasmic [NiFe]hydrogenase from
Desulfovibrio fructosovorans
JOURNAL Gene 94, 95-101 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Gene (1990) In
press] kindly submitted
by M.Rousset, 15-JUN-1990.
FEATURES Location/Qualifiers
misc_signal 97..102
/note="-35 region"
misc_signal 120..125
/note="-10 region"
mRNA 129..3060
/note="periplasmic hydrogenase mRNA"
misc_binding 289..293
/note="ribosome binding site for hydA"
sig_peptide 301..450
/note="periplasmic hydrogenase-alpha subunit signal
peptide"
CDS 301..1242
/note="periplasmic hydrogenase-alpha subunit precursor
(hydA) (EC 1.18.99,1)"
mat_peptide 451..1239
/note="periplasmic hydrogenase"
/codon_start=451
misc_binding 1303..1307
/note="ribosome binding site for hydB"
CDS 1314..3008
/note="periplasmic hydrogenase-beta subunit (hydB)"
misc_signal 3025..3060
/note="termination signal"
BASE COUNT 660 a 1150 c 866 g 504 t
ORIGIN
1 cgtgctgcat acgatttctt tattcatatc ctttacttcc ccggcctcat caccccggta
61 acacccttga atcacgtgaa aaatttgcca aaccccattg acgttcaaac aaactcgtga
121 ttaactgcaa aagggaaatc gaacccggcg acgcaggaac gcacgcgtcc gtcacataga
181 ggcggcggcg gccggttcgc ctgcgacggc cgcgtgccga acgggtcaac ggatgctacg
241 tggcctgggg atcatgggac gccccggacc atgcctctcg taaaacaaag gaggacgttt
301 atgaactttt ccgtgggtct tggcaggatg aatgcggaaa aacggcttgt gcaaaacggc
361 gtctcccgcc gcgacttcat gaaattttgc gccaccgtgg ccgcggccat gggcatgggc
421 ccggcgttcg cgcccaaggt cgccgaagca ttgacggcca aacaccgtcc gtcggtggtc
481 tggctgcaca acgccgagtg caccggctgc accgaagcgg cgatccggac gatcaaacct
541 tatatagacg cgctcattct cgacaccatc tccctggatt accaggagac catcatggcc
601 gcggccggcg agacgtccga ggcggccctg caccaggccc tcgaaggcaa ggacggctac
661 tacctcgtgg tcgagggcgg cctgcccacc atcgacggcg gccagtgggg catggttgcc
721 ggccatccca tgatcgagac caccaagaag gccgcggcca aggccaaggg catcatctgc
781 atccggcacc tgcctcacgg cggcgtccag aaggccaaac ccaatcccag ccaggccaag
841 ggcgtgtccg aagccctcgg cgtcaagacc atcaacatcc ccggctgccc gcccaacccc
901 atcaacttcg tgggcgccgt ggtccatgtc ctgaccaagg gcatcccgga tctcgacgag
961 aacggccgtc cgaagctctt ctacggcgag ctggtccacg acaactgtcc gcgcctgccc
1021 cacttcgagg cctccgaatt cgcgccctcc ttcgattccg aagaggccaa gaaaggcttc
1081 tgcctctacg aactcggctg caagggcccc gttacctaca acaactgccc caaggtgctg
1141 ttcaaccagg tcaactggcc cgtccaggcc ggccacccct gcctcggctg cagcgagccg
1201 gacttctggg acaccatgac gccgttctac gagcagggct aaccccctcc tttgtagcgg
1261 ccagtacccg gacaccttca acagcaccga acgtctcgtg acggaggaag catatggctg
1321 agagcaaacc cacgccacaa tccaccttca ccggccccat cgtggtcgac cccattaccc
1381 ggatcgaagg tcacttgcgg atcatggtcg aggtggaaaa cggtaaggtc aaggacgcct
1441 ggagctcctc gcagctcttc cgcggcctgg aaatcatcct caaaggccgc gatccccgcg
1501 acgcccagca cttcacccag cgcgcctgcg gcgtgtgcac gtacgtccac gccctggcct
1561 ccagccgctg cgtcgatgac gccgtcaagg tcagcatccc ggccaacgcc cgcatgatgc
1621 gcaacctggt catggcctcc cagtatctcc atgaccacct cgtccacttc tatcacctgc
1681 acgccctcga ctgggtcgac gtgaccgccg ccctcaaggc cgatcccaac aaggccgcca
1741 aactggcagc ctccatcgac acggctcgga ccggcaactc ggaaaaggcc ctcaaggcag
1801 tccaggacaa gctgaaagcc ttcgtcgagt ccggacagct cggcatcttc accaacgcct
1861 acttcctcgg cggccacaaa gcctactacc tgccgcccga ggtcaacctc atcgccaccg
1921 cccactacct ggaagccctg cacatgcagg tcaaggcggc cagcgccatg gccatcctcg
1981 gcggcaagaa cccccacacc cagttcaccg tcgtgggcgg ctgctccaac taccagggcc
2041 tgaccaagga cccgctggcc aactacctgg ccctgagcaa ggaagtctgc cagttcgtca
2101 acgagtgcta catccctgac ctgctggccg tggccggctt ctacaaggac tggggcggca
2161 tcggcggcac cagcaactac ctggccttcg gcgagttcgc caccgacgac agctcccccg
2221 agaaacacct ggccacctcg cagtttccgt ccggcgtcat caccggccgc gacctcggca
2281 aggtggataa cgtggacctc ggcgccatct acgaagacgt caagtactcc tggtacgccc
2341 ccggcggcga cggcaagcac ccctacgact gcgtcaccga tcccaagtac accaagctcg
2401 acgacaagga ccactactcc tggatgaagg ccccccgcta caagggcaag gccatggaag
2461 tcggtccctt ggcccgcacc ttcatcgcct acgccaaggg gcagcccgac ttcaaaaagg
2521 tcgtggacat ggtcctcggc aaactctccg tcccggccac ggccctgcat tcgaccctcg
2581 gacgcaccgc cgcccgcggc atcgagaccg ccatcgtctg cgccaacatg gagaagtgga
2641 tcaaggaaat ggccgacagc ggcgccaagg acaacaccct gtgcgccaag tgggagatgc
2701 ccgaggagtc caagggcgtc ggcctggccg atgctccccg cggctccctg tcccactgga
2761 tccgcatcaa gggcaagaag atcgacaact tccagctggt tgtcccctcg acctggaacc
2821 tcggtccccg ggggccccag ggcgacaaga gcccggtgga agaggccctt atcggcacgc
2881 ccatcgccga tcccaaacgc ccggtcgaaa tcctgcgcac ggtccacgcc ttcgacccct
2941 gcatcgcctg cggcgtgcac gtcatcgagc ccgagaccaa cgagatcctc aagttcaagg
3001 tttgctaaga gcggcaaccc tctaatacga aggacccgct tcgggccggg ttcttcgttt
3061 ctcaaggagg tcgcatgtcc gataccccgc gcaaaatcct catcctcggc ctcggcaaca
3121 tcctctacac cgacgagggc gtcggcgtgc gggccgtgga gcgccttctc gaaacccacg
//