GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS ECHPRTCA 1653 bp ds-DNA BCT 12-JAN-1991
DEFINITION E.chrysanthemi metalloprotease C (prtC) gene, complete cds.
ACCESSION M59229 J05627
KEYWORDS metalloprotease; protease C.
SOURCE E.chrysanthemi DNA.
ORGANISM Erwinia chrysanthemi
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1653)
AUTHORS Delepelaire,P. and Wandersman,C.
TITLE Protein secretion in gram-negative bacteria: The extracellular
metalloprotease B from Erwinia chrysanthemi contains a C-terminal
secretion signal analogous to that of Escherichia coli
alpha-hemolysin
JOURNAL J. Biol. Chem. 265, 17118-17125 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
RBS 130..134
/gene="prtC"
CDS 139..1578
/gene="prtC"
/codon_start=139
/product="metalloprotease C"
BASE COUNT 412 a 392 c 448 g 401 t
ORIGIN
1 aatattaaac agggtgggtt aaccatcctg tttaataaga tttccacggg aatcttatta
61 tcatgtcatt ctggcagggc gttgctcgtc ctgaataaag gatgacagta ttcttttatc
121 gtatttccag aggattttat gggaaaaaat ctgtctttac gtcaagatga cgctcaacac
181 gcattatccg cgaataccag cagcgcgtat aattccgttt atgattttct gcgttaccac
241 gatcgtggcg atggcctgac ggtcaatggc aaaacatcct attccatcga tcaggccgcg
301 gcgcaaatta cccgggaaaa tgtcagctgg aatggaacca atgtatttgg caagtcggcc
361 aatctgacgt ttaaatttct gcagtcggtc agctccattc catccggcga taccggtttt
421 gtgaaattta atgcagagca aattgagcag gcgaaactgt cgctgcaatc ctggtcggac
481 gtagccaatc tgacgtttac cgaagtaacg ggaaataaat ctgctaatat cacctttggt
541 aactatacgc gtgatgcgag tggcaacctg gattacggta ctcaagccta tgcctattat
601 ccgggtaatt atcagggggc gggcagcagc tggtacaact ataaccagtc taatatccgt
661 aatccggggt cggaagaata tggccgtcag acctttaccc acgagattgg tcatgcgctt
721 ggcctggccc atccgggtga gtacaacgcc ggcgaaggtg acccgagcta taatgacgcg
781 gtgtatgcgg aagatagcta tcaattcagt atcatgagct actggggcga gaatgaaacc
841 ggtgccgact acaacggtca ctatggcggc gcgccgatga ttgacgacat cgcggcgatt
901 cagcggctgt atggcgccaa catgaccacc cgtaccggtg atagcgtgta tggtttcaat
961 tccaataccg atcgtgattt ctatactgcg accgacagca gtaaagcact gattttctca
1021 gtatgggatg ccggcggcac cgacaccttc gacttctccg gctatagcaa caaccagcgt
1081 atcaatctga atgaaggctc gttttcggac gtgggcggcc tgaaaggcaa cgtgtcgatc
1141 gcgcacggcg tgaccatcga aaacgccatt ggcggttccg gcaacgatat tctggtgggc
1201 aacagcgcag ataacatact gcagggcggc gcgggcaatg acgtgctgta cggtggtgcc
1261 ggcgcggata cgctgtacgg cggcgccggg cgtgatacgt tcgtttacgg cagcggtcag
1321 gattccaccg tggcagcgta tgactggatt gccgatttcc agaaaggcat tgataaaatc
1381 gacctgtcgg cgttccgcaa cgaaggtcaa ctgagctttg tgcaggatca gttcaccggc
1441 aaaggtcagg aagtgatgct gcagtgggat gcggccaaca gcatcaccaa cctgtggttg
1501 catgaagcag ggcacagctc ggtagacttc ctggtacgaa ttgtcggtca ggcggcacag
1561 tccgatatta ttgtgtaatg atattctgaa aatattgcac agggcagttt atctgccctg
1621 tttaatattc cattctgcat tagggtagaa ttc
//GenBank-Updates@genbank.bio.net (01/12/91)
LOCUS ECHPRTC 2164 bp ds-DNA BCT 12-JAN-1991
DEFINITION E.chrysanthemi metalloprotease C (prtC) gene, complete cds.
ACCESSION M37390
KEYWORDS metalloprotease; protease C.
SOURCE E.chrysanthemi (strain EC16) DNA.
ORGANISM Erwinia chrysanthemi
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dahler,G.S., Barras,F. and Keen,N.T.
TITLE Cloning of genes encoding extracellular metalloproteases from
Erwinia chrysanthemi EC16
JOURNAL J. Bacteriol. 172, 5803-5815 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol.
172, 5803-5815 (1990)] kindly submitted by N.T.Keen, 01-AUG-1990.
FEATURES Location/Qualifiers
-35_signal 171..176
/gene="prtC"
-10_signal 194..199
/gene="prtC"
CDS 348..1784
/gene="prtC"
/codon_start=348
/product="metalloprotease C"
misc_signal 1794..1813
/note="transcription termination signal"
/gene="prtC"
BASE COUNT 559 a 479 c 523 g 603 t
ORIGIN
1 attatttttt ttgaagcaat tccggatgag tataatcatc tttgtttctg cgcaatcgtt
61 ttcttttttt tgccccattc gaataactta tttcactttc atcaattagc aatatactaa
121 aaacaaaaaa ataatttatt gaataaaatg aaaaaaataa cgtacttttc tttacaaaaa
181 cattacggtg acttatataa atagtcacgc tattaatagc gctgattgca ttcacccaat
241 atcactggat ttctacagga aatatgtcat taaattggca aggcgttatt tacctgtcga
301 tttaatggtg atggggttct ctggttatta acgtcaatga gggttttatg gaaaaaaatc
361 tgtcttcacg tgatgatgac gccttacatt cattatctgc gcccagcagc agctataact
421 cgatttatga cctgttgcat tatcatgagc gtggtaatgg attgacgatt aacggtaaac
481 catcctattc aattgaggat gcgggagatc aaattacccg tgataacgtc agttggaatg
541 gagccaatgt atttggcaag tcggctaatc tgactttcaa attccttcag tctgcacgtt
601 ccactccgga tggcgatacc ggtttcgtca agtttaatgc ggcgcaaatt tctcaggcca
661 aactggcatt acaatcctgg gcggatgtcg ctaacgtcac ctttacggaa gtcacgggta
721 atcagtcggc caatgtcacc ttcggtaact atacccgtga ttccagcggc cgcctggatt
781 atggcacgca agcgtatgct tatctgccgg ggagtggtag cgcgtcgggg acgacctggt
841 acaactataa tgtggataac attcgtagcc cggataccat ggaatacggc cgtcagacgt
901 tgacccatga aattggccac gcgttgggct tgaaccatcc gggagactat aacgccggcg
961 agggtaaccc cagttacagt gatgtgacct acgccgaaga cacccgtcag ttcagtatca
1021 tgagctactg gagcgaaaaa aataccggcg gggatttcaa aggtcattac gccgccggcc
1081 cgatgctgga tgatatcgcc gctatccagc gcctgtacgg cgccaatatg acgacccgta
1141 ccggtgattc ggtttatggt ttcaactcca ataccgatcg tgatttttac accgcgacca
1201 gcagtagcaa agcgctgatt ttctcggcgt gggatgcggg tggcaatgat acctttgatt
1261 tctcgggtta cagcaataac cagcgcatta acctgaacga tggctcactc tcggatgtcg
1321 gcggcctgaa aggcaatgta tcgattgccg agggcgtgac gattgaaaac gcgattggcg
1381 gctccggtaa tgatttgctg attggtaata atgctgacaa tactctgcgt ggcggggcag
1441 gtgatgatgt tctgttcggt ggaagcgggg ctgatcgact gtacggcggc agtggccgag
1501 atacctttgt gtacacggcg gcgtccgact ccaaggtcgc ggcacccgat tggcttctcg
1561 attttcagac cggggccgat aaaatcgatc tctctgcact gaataccggc aataatctgc
1621 attttgttaa ccaattcagc ggtagtggcg gtgagatcat gctcaactgg gatgcttctg
1681 ccaacaccag caatctttat ctgaatctgg ataacaacac ttctcctgag tttttggtga
1741 agattgtcgg ccaggtatct cagacggctg attttgttgt ttaaactgca tcgaagggcg
1801 ggtctccgcc ctttattttc ccttttaggt taaccatgtt acgctggttt tctaaatcaa
1861 tttagttaca acatacatat gtaatataaa caccactcgt ctgaaacaac cgcgttttta
1921 ttacactacc gagaaaacaa catttgaaca ggtcgaaggg ggactgcgat ggtgcaggct
1981 ttctcgttgt cataacggaa agaggaaaag ggttaccgat tggctaagat aacccttcag
2041 cgttggagac gtttacaggt tagggcagtt ggactttacc ggtgatcaga taggctgacg
2101 acgtactgtc cgacatcggc ggtaacgtat cgcttaatat attgccttta atatcggtga
2161 attc
//