GenBank-Updates@genbank.bio.net (01/08/91)
LOCUS NOTAFGP 4447 bp ds-DNA VRT 08-JAN-1991
DEFINITION N.coriiceps neglecta antifreeze glycoprotein gene, complete cds.
ACCESSION M55000
KEYWORDS antifreeze glycopeptide; antifreeze glycoprotein.
SOURCE N.coriiceps neglecta testis DNA.
ORGANISM
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Osteichthyes;
Actinopterygii; Perciformes; ; .
REFERENCE 1 (bases 1 to 4447)
AUTHORS Hsiao,K.-C., Cheng,C.-H.C., Fernandes,I.E., Detrich,W.H. and
DeVries,A.L.
TITLE An antifreeze glycopeptide gene from the antarctic cod Notothenia
coriiceps neglecta encodes a polyprotein of high peptide copy
number
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9265-9269 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CAAT_signal 519..522
/gene="AFGP8"
TATA_signal 529..534
/gene="AFGP8"
5'UTR 578..620
/gene="AFGP8"
CDS 621..3089
/gene="AFGP8"
/codon_start=621
/product="antifreeze glycoprotein"
sig_peptide 621..731
/gene="AFGP8"
/codon_start=621
mat_peptide 732..3086
/note="AFGP8 polyprotein contains 44 copies of AFGP8 and 2
copies of AFGP7. Post translational cleavage to produce 46
individual peptides"
/gene="AFGP8"
/codon_start=732
/product="antifreeze glycoprotein"
repeat_region 732..3086
/note="46 tandem repeats each coding for 1 peptide and a 3
residue spacer"
/rpt_type=tandem
polyA_signal 3330..3335
/gene="AFGP8"
BASE COUNT 1242 a 1167 c 975 g 1063 t
ORIGIN
1 aaaaatgtat tttgacttct gaataattta attgttatat gtgtagacat ataaatcatt
61 tgtcaaaaca gggctacatt tgattaaatt ataaaaaata tgtggtcaaa tgaagagcgg
121 tttgggagcc gaaagagccg gctcttcgtg gtgagccgag ccaaatgatc cagctcacta
181 aaaagagacg gaattcccat cacgacacac aactgctgac tcggcgcagc accggcaatt
241 taactgctgg gagaaaaact gactaatcag atgtaaacgt taatctttga taatatttag
301 tctcctcctt tttcgtcaac taaagtagag agaaattctg tcaaagttct tatttagtaa
361 actacatttt cgtttcatca cttttcgtca acgatattgc atgttgattt cgttacagtt
421 attgtttact gacgtcggtg ccgtcaggtc atcgtctcgt cttagtcatg gaaaaaaagg
481 ttgttctcga acatattttg tcatagtttt agtcgacgca attaacacta taatacacac
541 attcacaaag atacacacac aaacagtaaa cagtacatag aaaacatact gtagtgtaag
601 atattaaata ctaaatttga atgtttgtgg ttgaggataa caaatgtatc tctttctctg
661 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtctgtgtg tgtgtgtgaa acagctgtaa
721 cagctgcacc agctgcaaca gctgcaacag ctgcaacacc tgcaacagct gcattgaatt
781 ttgctgcaac agcggcaaca cctgcaacac cggcaacacc tgcattgatt tttgctgcaa
841 cagctgcaac ggcggcaacc cctgcaacag ctgcattgaa ttttgctgca acagcggcaa
901 cccctgcaac agctgcaacg cctgcattga tttttgctgc agcagctgca acagcggcaa
961 cccctgcaac agctgcattg aattttgctg caacagcggc aacacctgca acagcggcaa
1021 cacctgcatt gatttttgct gcaacagctg caacggcggc aacccctgca acacctgcat
1081 ttcattttgc tgcaacagcg gcaacccctg caacagctgc aacacctgca ttgatttttg
1141 ctgcaacagc tgcaacggcg gcaacccctg caacacctgc atttcatttt gctgcaacag
1201 cggcaacccc tgcaacagct gcaacccctg cattgatttt tgctgcaaca gctgcaacgg
1261 cggcaacccc tgcaacagct gcattgaatt ttgctgcaac agcggcaacc cctgcaacag
1321 ctgcaacacc tgcattgatt tttgctgcaa cagctgcaac agcggcaacc cctgcaacag
1381 ctgcattgaa ttttgctgca acagctgcaa cggcggcaac ccctgcaaca gctgcatgta
1441 attttgctgc aacagcggca acacctgcaa cagcggcaac acctgcattg atttttgctg
1501 caacagctgc aacggcggca acccctgcaa cagctgcatg taattttgct gcaacagcgg
1561 caacacctgc aacagcggca acacctgcat tgatttttgc tgcaacagct gcaacggcgg
1621 caacccctgc aacagctgca ttgaattttg ctgcaacagc ggcaacccct gcaacagctg
1681 caacgcctgc attgattttt gctgcaacag ctgcaacggc ggcaacccct gcaacagctg
1741 cattgaattt tgctgcaaca gcggcaaccc ctgcaacagc tgcaacgcct gcattgattt
1801 ttgctgcgac agctgcaacg gcggcaaccc ctgcaacagc tgcattgaat tttgctgcaa
1861 cagctgcaac ggcggcaacc cctgcaacac ctgcatttaa ttttgctgca acagcggcaa
1921 cccctgcaac agctgcaaca cctgcattga tttttgctgc aacagctgca acggcggcaa
1981 cccctgcaac agctgcattg aattttgctg caacagcggc aacacctgca acagctgcaa
2041 cacctgcatt gatttttgct gcaacagctg caacggcggc aacccctgca acagctgcat
2101 tgcattttgc tgcaacagct gcaacggcgg caacccctgc aacagctgca ttgaattttg
2161 ctgcaacagc ggcaacccct gcaacagctg caacacctgc attgattttt gctgcaacag
2221 ctgcaacggc ggcaacccct gcaacagctg cattcaattt tgctgcaaca gctgcaacgg
2281 cggcaacccc tgcaacagct gcattgaatt ttgctgcaac agcggcaacc cctgcaacag
2341 ctgcaacgcc tgcattgatt tttgctgcga cagctgcaac ggcggcaacc cctgcaacag
2401 ctgcattgaa ttttgctgca acagcggcaa cccctgcaac agctgcaaca cctgcattga
2461 tttttgctgc aacagctgca acggcggcaa cccctgcaac acctgcattt cattttgctg
2521 caacagcggc aacccctgca acagctgcaa cacctgcatt gatttttgct gcaacagctg
2581 caacggcggc aacccctgca acagctgcat tgaattttgc tgcaacagct gcaacggcgg
2641 caacccctgc aacagctgca ttgaattttg ctgcaacagc ggcaacccct gcaacagctg
2701 caacgcctgc attgattttt gctgcgacag ctgcaacggc ggcaacccct gcaacagctg
2761 cattgaattt tgctgcaaca gcggcaacac ctgcaacagc ggcaacgcct gcattgattt
2821 ttgctgcgac agctgcaacg gcggcaaccc ctgcaacagc tgcatttaat tttgctgcaa
2881 cagctgcaac ggcggcaacc cctgcaacag ctgcaacacc tgcattgatt tttgctgcca
2941 cagctgcaac agcggcaacc cctgcaacac ctgcaacacc tgcattgatt tttgctgcca
3001 cagctgcaac agcggcaacc cctgcaacac ctgcattgaa ttttgctgcc acagctgcaa
3061 cagcggcaac aacagcagca agaggttgaa tctggcagcc ctctggtgtg tgacggagag
3121 gtgacggcct ggtgtcatgg ggtcagggat atgccgagct tttctacccc cactcctggt
3181 ttgaagaggt cctggcagcc aatcccgaaa gaaaatacca gtttattttc ttcacttctc
3241 ccacgtttca cagccctgtt ttatttttct gtctttctct ggtgccaatc aagactctta
3301 acacacatgt gccagaatgt tgaaaactaa ataaaataac ttgctattca cttatctgtc
3361 tataatgtga aaacatacac caatcactta tacgctcaag tatgtgttag gtcacaggtt
3421 tatgtatttg atttaatacc atttaagaca gtttgatggt gctctatcgc tttatataca
3481 gtagagcagg gttcccaaag tgtgaggggc cccgtcctgg gggcgccaga gagcttcaag
3541 ggatgagccg ccaaaaagag gaagaagaga aacagtgaaa tgtagctagt taacttcagc
3601 taaatgtatg cgagctggaa aatatggata gatttttagt gaagttacct aaagcgagag
3661 aggagacaga gtctgtcaga aaacggatga agtgtgggac cggtctgatg aactcgtgtg
3721 ttatctggaa ggagggtact agataatgac gtatgagacc atgtaaactc cggggtcata
3781 ttgagacgcc ccaccatgtt tggtgactaa gccacaggag ttttttgacc ggaagctaaa
3841 ggaattgcag tcacagaaaa atgtattgac acatttagca ctgtgaatac aaaggcaacc
3901 gaagcatcat accgagaggc gttacggata gctaaggcag gtaaacttca caacattggt
3961 gaaacgttac tcctccctgc tgctaaagac atctccgtcg aacctattca tctccgagtg
4021 gcaacgtatg aggaagctcg acgtatgcta gcagtaactc acacatggtt gaacgtactc
4081 ctcctgctgc tagcatctcg tcgaacctat tcatctccga gtgcaaacgt atgaggaagc
4141 tgccatcctt aacctcaaca tgtttaaaac ataaagataa agaggtttaa aaaagctatt
4201 gtttaatgtt ttaaggaggt gacgctcagc caatgaaaaa agagaaaggt agcgaaaaca
4261 gagcatgaaa tgttctctta ctggacctct ttgaaatgca attgaatacc ctgcattata
4321 gcaacaacaa caacaataat aataataata ataataataa tagcaataat aattgggagg
4381 aggggcacgg ctgtgattat gttctctgag gggaggctca ccttcccaca ctttgggaac
4441 cctgcag
//GenBank-Updates@genbank.bio.net (01/15/91)
LOCUS NOTAFGP 4447 bp ds-DNA VRT 14-JAN-1991
DEFINITION N.coriiceps neglecta antifreeze glycoprotein gene, complete cds.
ACCESSION M55000
KEYWORDS antifreeze glycopeptide; antifreeze glycoprotein.
SOURCE N.coriiceps neglecta testis DNA.
ORGANISM
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Osteichthyes;
Actinopterygii; Perciformes; ; .
REFERENCE 1 (bases 1 to 4447)
AUTHORS Hsiao,K.-C., Cheng,C.-H.C., Fernandes,I.E., Detrich,W.H. and
DeVries,A.L.
TITLE An antifreeze glycopeptide gene from the antarctic cod Notothenia
coriiceps neglecta encodes a polyprotein of high peptide copy
number
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9265-9269 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CAAT_signal 519..522
/gene="AFGP8"
TATA_signal 529..534
/gene="AFGP8"
5'UTR 578..620
/gene="AFGP8"
CDS 621..3089
/gene="AFGP8"
/codon_start=621
/product="antifreeze glycoprotein"
sig_peptide 621..731
/gene="AFGP8"
/codon_start=621
mat_peptide 732..3086
/note="AFGP8 polyprotein contains 44 copies of AFGP8 and 2
copies of AFGP7. Post translational cleavage to produce 46
individual peptides"
/gene="AFGP8"
/codon_start=732
/product="antifreeze glycoprotein"
repeat_region 732..3086
/note="46 tandem repeats each coding for 1 peptide and a 3
residue spacer"
/rpt_type=tandem
polyA_signal 3330..3335
/gene="AFGP8"
BASE COUNT 1242 a 1167 c 975 g 1063 t
ORIGIN
1 aaaaatgtat tttgacttct gaataattta attgttatat gtgtagacat ataaatcatt
61 tgtcaaaaca gggctacatt tgattaaatt ataaaaaata tgtggtcaaa tgaagagcgg
121 tttgggagcc gaaagagccg gctcttcgtg gtgagccgag ccaaatgatc cagctcacta
181 aaaagagacg gaattcccat cacgacacac aactgctgac tcggcgcagc accggcaatt
241 taactgctgg gagaaaaact gactaatcag atgtaaacgt taatctttga taatatttag
301 tctcctcctt tttcgtcaac taaagtagag agaaattctg tcaaagttct tatttagtaa
361 actacatttt cgtttcatca cttttcgtca acgatattgc atgttgattt cgttacagtt
421 attgtttact gacgtcggtg ccgtcaggtc atcgtctcgt cttagtcatg gaaaaaaagg
481 ttgttctcga acatattttg tcatagtttt agtcgacgca attaacacta taatacacac
541 attcacaaag atacacacac aaacagtaaa cagtacatag aaaacatact gtagtgtaag
601 atattaaata ctaaatttga atgtttgtgg ttgaggataa caaatgtatc tctttctctg
661 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtctgtgtg tgtgtgtgaa acagctgtaa
721 cagctgcacc agctgcaaca gctgcaacag ctgcaacacc tgcaacagct gcattgaatt
781 ttgctgcaac agcggcaaca cctgcaacac cggcaacacc tgcattgatt tttgctgcaa
841 cagctgcaac ggcggcaacc cctgcaacag ctgcattgaa ttttgctgca acagcggcaa
901 cccctgcaac agctgcaacg cctgcattga tttttgctgc agcagctgca acagcggcaa
961 cccctgcaac agctgcattg aattttgctg caacagcggc aacacctgca acagcggcaa
1021 cacctgcatt gatttttgct gcaacagctg caacggcggc aacccctgca acacctgcat
1081 ttcattttgc tgcaacagcg gcaacccctg caacagctgc aacacctgca ttgatttttg
1141 ctgcaacagc tgcaacggcg gcaacccctg caacacctgc atttcatttt gctgcaacag
1201 cggcaacccc tgcaacagct gcaacccctg cattgatttt tgctgcaaca gctgcaacgg
1261 cggcaacccc tgcaacagct gcattgaatt ttgctgcaac agcggcaacc cctgcaacag
1321 ctgcaacacc tgcattgatt tttgctgcaa cagctgcaac agcggcaacc cctgcaacag
1381 ctgcattgaa ttttgctgca acagctgcaa cggcggcaac ccctgcaaca gctgcatgta
1441 attttgctgc aacagcggca acacctgcaa cagcggcaac acctgcattg atttttgctg
1501 caacagctgc aacggcggca acccctgcaa cagctgcatg taattttgct gcaacagcgg
1561 caacacctgc aacagcggca acacctgcat tgatttttgc tgcaacagct gcaacggcgg
1621 caacccctgc aacagctgca ttgaattttg ctgcaacagc ggcaacccct gcaacagctg
1681 caacgcctgc attgattttt gctgcaacag ctgcaacggc ggcaacccct gcaacagctg
1741 cattgaattt tgctgcaaca gcggcaaccc ctgcaacagc tgcaacgcct gcattgattt
1801 ttgctgcgac agctgcaacg gcggcaaccc ctgcaacagc tgcattgaat tttgctgcaa
1861 cagctgcaac ggcggcaacc cctgcaacac ctgcatttaa ttttgctgca acagcggcaa
1921 cccctgcaac agctgcaaca cctgcattga tttttgctgc aacagctgca acggcggcaa
1981 cccctgcaac agctgcattg aattttgctg caacagcggc aacacctgca acagctgcaa
2041 cacctgcatt gatttttgct gcaacagctg caacggcggc aacccctgca acagctgcat
2101 tgcattttgc tgcaacagct gcaacggcgg caacccctgc aacagctgca ttgaattttg
2161 ctgcaacagc ggcaacccct gcaacagctg caacacctgc attgattttt gctgcaacag
2221 ctgcaacggc ggcaacccct gcaacagctg cattcaattt tgctgcaaca gctgcaacgg
2281 cggcaacccc tgcaacagct gcattgaatt ttgctgcaac agcggcaacc cctgcaacag
2341 ctgcaacgcc tgcattgatt tttgctgcga cagctgcaac ggcggcaacc cctgcaacag
2401 ctgcattgaa ttttgctgca acagcggcaa cccctgcaac agctgcaaca cctgcattga
2461 tttttgctgc aacagctgca acggcggcaa cccctgcaac acctgcattt cattttgctg
2521 caacagcggc aacccctgca acagctgcaa cacctgcatt gatttttgct gcaacagctg
2581 caacggcggc aacccctgca acagctgcat tgaattttgc tgcaacagct gcaacggcgg
2641 caacccctgc aacagctgca ttgaattttg ctgcaacagc ggcaacccct gcaacagctg
2701 caacgcctgc attgattttt gctgcgacag ctgcaacggc ggcaacccct gcaacagctg
2761 cattgaattt tgctgcaaca gcggcaacac ctgcaacagc ggcaacgcct gcattgattt
2821 ttgctgcgac agctgcaacg gcggcaaccc ctgcaacagc tgcatttaat tttgctgcaa
2881 cagctgcaac ggcggcaacc cctgcaacag ctgcaacacc tgcattgatt tttgctgcca
2941 cagctgcaac agcggcaacc cctgcaacac ctgcaacacc tgcattgatt tttgctgcca
3001 cagctgcaac agcggcaacc cctgcaacac ctgcattgaa ttttgctgcc acagctgcaa
3061 cagcggcaac aacagcagca agaggttgaa tctggcagcc ctctggtgtg tgacggagag
3121 gtgacggcct ggtgtcatgg ggtcagggat atgccgagct tttctacccc cactcctggt
3181 ttgaagaggt cctggcagcc aatcccgaaa gaaaatacca gtttattttc ttcacttctc
3241 ccacgtttca cagccctgtt ttatttttct gtctttctct ggtgccaatc aagactctta
3301 acacacatgt gccagaatgt tgaaaactaa ataaaataac ttgctattca cttatctgtc
3361 tataatgtga aaacatacac caatcactta tacgctcaag tatgtgttag gtcacaggtt
3421 tatgtatttg atttaatacc atttaagaca gtttgatggt gctctatcgc tttatataca
3481 gtagagcagg gttcccaaag tgtgaggggc cccgtcctgg gggcgccaga gagcttcaag
3541 ggatgagccg ccaaaaagag gaagaagaga aacagtgaaa tgtagctagt taacttcagc
3601 taaatgtatg cgagctggaa aatatggata gatttttagt gaagttacct aaagcgagag
3661 aggagacaga gtctgtcaga aaacggatga agtgtgggac cggtctgatg aactcgtgtg
3721 ttatctggaa ggagggtact agataatgac gtatgagacc atgtaaactc cggggtcata
3781 ttgagacgcc ccaccatgtt tggtgactaa gccacaggag ttttttgacc ggaagctaaa
3841 ggaattgcag tcacagaaaa atgtattgac acatttagca ctgtgaatac aaaggcaacc
3901 gaagcatcat accgagaggc gttacggata gctaaggcag gtaaacttca caacattggt
3961 gaaacgttac tcctccctgc tgctaaagac atctccgtcg aacctattca tctccgagtg
4021 gcaacgtatg aggaagctcg acgtatgcta gcagtaactc acacatggtt gaacgtactc
4081 ctcctgctgc tagcatctcg tcgaacctat tcatctccga gtgcaaacgt atgaggaagc
4141 tgccatcctt aacctcaaca tgtttaaaac ataaagataa agaggtttaa aaaagctatt
4201 gtttaatgtt ttaaggaggt gacgctcagc caatgaaaaa agagaaaggt agcgaaaaca
4261 gagcatgaaa tgttctctta ctggacctct ttgaaatgca attgaatacc ctgcattata
4321 gcaacaacaa caacaataat aataataata ataataataa tagcaataat aattgggagg
4381 aggggcacgg ctgtgattat gttctctgag gggaggctca ccttcccaca ctttgggaac
4441 cctgcag
//