GenBank-Updates@genbank.bio.net (01/08/91)
LOCUS ECOMTR 1867 bp ds-DNA BCT 08-JAN-1991
DEFINITION E.coli tryptophan-specific permease (mtr) gene, complete cds.
ACCESSION M59862 M35417
KEYWORDS 5-methyltryptophan resistance; membrane protein; mtr gene;
tryptophan permease; tryptophan-specific permease.
SOURCE E.coli K-12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1867)
AUTHORS Heatwole,V.M. and Somerville,R.L.
TITLE Cloning, nucleotide sequence, and characterization of mtr, the
structural gene for a tryptophan-specific permease of Escherichia
coli K-12
JOURNAL J. Bacteriol. 173, 108-115 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
protein_bind 313..334
/bound_moiety="TyrR regulatory protein"
/standard_name="putative TyrR operator"
/gene="mtr"
-35_signal 327..332
/gene="mtr"
protein_bind 343..364
/note="TyrR operator"
/bound_moiety="TyrR regulatory protein"
/standard_name="putative TyrR operator"
/gene="mtr"
-10_signal 350..355
/gene="mtr"
protein_bind 400..421
/bound_moiety="TrpR regulatory protein"
/standard_name="putative TrpR operator"
/gene="mtr"
CDS 473..1717
/gene="mtr"
/codon_start=473
/product="tryptophan-specific permease"
BASE COUNT 356 a 463 c 525 g 523 t
ORIGIN Map position 3372 kb Kohara coordinates.
1 tgtttgcttc tcactccacc atcgaactgc cgaaagtatg ccgggtgaag tgctgctaca
61 ctttacgcgc actcgcattc tcaacaagcc gatgaacatg cagttactgg gcgatgcaca
121 gccgcatact ggcggtgagc gtcgtggcgg tggtcgtggt ttcggtggcg aacgtcgtga
181 aggcggtcgt aacttcagcg gtgaacgccg tgaaggtggc cgtggtgatg gtcgtcgttt
241 tagcggcgaa cgtcgtgaag gccgcgctcc gcgtcgtgat gattctaccg gtcgtcgtcg
301 tttcggtggt gatgcgtaat catgcctgaa cagcgaacac aatctgtaaa ataatatata
361 cagccccgat ttttaccatc ggggcttttt ttctgtcttt tgtactcgtg tactggtaca
421 gtgcaatgca taacaacgca gtcgcactat ttttcactgg agagaagccc tcatggcaac
481 actaaccacc acccaaacgt caccgtcgct gcttggcggc gtggtgatta tcggcggcac
541 cattattggc gcagggatgt tttctctgcc agtggtcatg tccggggcgt ggtttttctg
601 gtcaatggcg gcgctgatct ttacctggtt ctgtatgctg cattccggct tgatgattct
661 ggaagctaac ctgaattaca gaatcggttc gagttttgac accatcacca aagatttgct
721 gggcaaaggc tggaacgtgg tcaacggcat ttccattgcc tttgtgctct atatcctgac
781 ctatgcctat atttctgcca gtggttcgat tctgcatcac accttcgcag agatgtcact
841 aaacgtcccg gcacgggcgg cgggttttgg ttttgcattg ctggtagcgt ttgtggtgtg
901 gttgagcact aaagccgtca gtcgcatgac agcgattgtg ctgggggcga aagtcattac
961 cttcttcctc acctttggta gcctgctggg gcatgtgcag cctgcgacat tgttcaacgt
1021 cgccgaaagc aatgcgtctt atgcaccgta tctgttgatg accctgccgt tctgtctggc
1081 atcgtttggt tatcacggta acgtgccaag cctgatgaag tattacggca aagatccgaa
1141 aaccatcgtg aaatgtctgg tgtacggtac gctgatggcg ctggcgctgt ataccatctg
1201 gttgctggcg acgatgggta acatcccgcg tccggagttt atcggtattg cagagaaggg
1261 cggtaatatt gatgtgctgg tacaggcgtt aagcggcgta ctgaacagcc gtagtctgga
1321 tctgctgctg gtcgtgttct caaactttgc ggtagcgagt tcgttcctcg gcgtaacgct
1381 gggtttgttt gactatctgg cagatctgtt tggtttcgac gactcggctg tgggccgctt
1441 gaaaacggca ttgctgacct ttgccccgcc agttgtgggg gggctgttgt tcccgaacgg
1501 attcctgtac gccattggtt atgctggttt agcggctacc atctgggcgg caattgttcc
1561 ggcgctgtta gcccgtgcat cgcgtaaacg ctttggcagc ccgaaattcc gcgtctgggg
1621 tggcaagccg atgattgcgc tgattctggt gtttggcgtc ggcaacgcac tggtgcatat
1681 tttatcgagc tttaatttac tgccggtgta tcagtaatca gcggtgcctt atccgacatt
1741 tctgctgcct acacaatgct gatcgttcgc ttatcaggtc tatgtaggac agcgttgcca
1801 gctcggataa ggcttcccgc gttaagacac actatcccaa caactcttcc ttaacatcca
1861 tcgccag
//GenBank-Updates@genbank.bio.net (01/15/91)
LOCUS ECOMTR 1867 bp ds-DNA BCT 14-JAN-1991
DEFINITION E.coli tryptophan-specific permease (mtr) gene, complete cds.
ACCESSION M59862 M35417
KEYWORDS 5-methyltryptophan resistance; membrane protein; mtr gene;
tryptophan permease; tryptophan-specific permease.
SOURCE E.coli K-12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1867)
AUTHORS Heatwole,V.M. and Somerville,R.L.
TITLE Cloning, nucleotide sequence, and characterization of mtr, the
structural gene for a tryptophan-specific permease of Escherichia
coli K-12
JOURNAL J. Bacteriol. 173, 108-115 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
protein_bind 313..334
/bound_moiety="TyrR regulatory protein"
/standard_name="putative TyrR operator"
/gene="mtr"
-35_signal 327..332
/gene="mtr"
protein_bind 343..364
/note="TyrR operator"
/bound_moiety="TyrR regulatory protein"
/standard_name="putative TyrR operator"
/gene="mtr"
-10_signal 350..355
/gene="mtr"
protein_bind 400..421
/bound_moiety="TrpR regulatory protein"
/standard_name="putative TrpR operator"
/gene="mtr"
CDS 473..1717
/gene="mtr"
/codon_start=473
/product="tryptophan-specific permease"
BASE COUNT 356 a 463 c 525 g 523 t
ORIGIN Map position 3372 kb Kohara coordinates.
1 tgtttgcttc tcactccacc atcgaactgc cgaaagtatg ccgggtgaag tgctgctaca
61 ctttacgcgc actcgcattc tcaacaagcc gatgaacatg cagttactgg gcgatgcaca
121 gccgcatact ggcggtgagc gtcgtggcgg tggtcgtggt ttcggtggcg aacgtcgtga
181 aggcggtcgt aacttcagcg gtgaacgccg tgaaggtggc cgtggtgatg gtcgtcgttt
241 tagcggcgaa cgtcgtgaag gccgcgctcc gcgtcgtgat gattctaccg gtcgtcgtcg
301 tttcggtggt gatgcgtaat catgcctgaa cagcgaacac aatctgtaaa ataatatata
361 cagccccgat ttttaccatc ggggcttttt ttctgtcttt tgtactcgtg tactggtaca
421 gtgcaatgca taacaacgca gtcgcactat ttttcactgg agagaagccc tcatggcaac
481 actaaccacc acccaaacgt caccgtcgct gcttggcggc gtggtgatta tcggcggcac
541 cattattggc gcagggatgt tttctctgcc agtggtcatg tccggggcgt ggtttttctg
601 gtcaatggcg gcgctgatct ttacctggtt ctgtatgctg cattccggct tgatgattct
661 ggaagctaac ctgaattaca gaatcggttc gagttttgac accatcacca aagatttgct
721 gggcaaaggc tggaacgtgg tcaacggcat ttccattgcc tttgtgctct atatcctgac
781 ctatgcctat atttctgcca gtggttcgat tctgcatcac accttcgcag agatgtcact
841 aaacgtcccg gcacgggcgg cgggttttgg ttttgcattg ctggtagcgt ttgtggtgtg
901 gttgagcact aaagccgtca gtcgcatgac agcgattgtg ctgggggcga aagtcattac
961 cttcttcctc acctttggta gcctgctggg gcatgtgcag cctgcgacat tgttcaacgt
1021 cgccgaaagc aatgcgtctt atgcaccgta tctgttgatg accctgccgt tctgtctggc
1081 atcgtttggt tatcacggta acgtgccaag cctgatgaag tattacggca aagatccgaa
1141 aaccatcgtg aaatgtctgg tgtacggtac gctgatggcg ctggcgctgt ataccatctg
1201 gttgctggcg acgatgggta acatcccgcg tccggagttt atcggtattg cagagaaggg
1261 cggtaatatt gatgtgctgg tacaggcgtt aagcggcgta ctgaacagcc gtagtctgga
1321 tctgctgctg gtcgtgttct caaactttgc ggtagcgagt tcgttcctcg gcgtaacgct
1381 gggtttgttt gactatctgg cagatctgtt tggtttcgac gactcggctg tgggccgctt
1441 gaaaacggca ttgctgacct ttgccccgcc agttgtgggg gggctgttgt tcccgaacgg
1501 attcctgtac gccattggtt atgctggttt agcggctacc atctgggcgg caattgttcc
1561 ggcgctgtta gcccgtgcat cgcgtaaacg ctttggcagc ccgaaattcc gcgtctgggg
1621 tggcaagccg atgattgcgc tgattctggt gtttggcgtc ggcaacgcac tggtgcatat
1681 tttatcgagc tttaatttac tgccggtgta tcagtaatca gcggtgcctt atccgacatt
1741 tctgctgcct acacaatgct gatcgttcgc ttatcaggtc tatgtaggac agcgttgcca
1801 gctcggataa ggcttcccgc gttaagacac actatcccaa caactcttcc ttaacatcca
1861 tcgccag
//