GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS TIPNPSS 6425 bp ds-DNA SYN 16-JAN-1991
DEFINITION A.tumefaciens T-DNA vector containing octopine T-DNA borders and
markers: neomycin-phosphotransferase - octopine synthase (3' end)
and Sp/Sm adenyltransferase. complete cds.
ACCESSION M35007
KEYWORDS neomycin phosphotransferase;
streptomycin/spectinomycin adenyltransferase.
SOURCE N.tabacum T-DNA inserts in A.tumefaciens DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 6425)
AUTHORS Gheysen,G., Herman,L., Breyne,P., Gielen,J., Van Montagu,M. and
Depicker,A.
TITLE Cloning ans sequence analysis of truncated T-DNA inserts from
Nicotiana tabacum
JOURNAL Gene 94, 155-163 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Gene (1990) In
press] kindly submitted
by G.D.R.Gheysen, 01-JUN-1990.
FEATURES Location/Qualifiers
misc_feature 1..24
/note="left T-DNA border"
misc_recomb 24^25
/note="T-DNA end/plant DNA start"
misc_recomb 40^41
/note="plant DNA end/T-DNA start"
misc_feature 372..395
/note="24 bp border-like sequence"
misc_recomb 1094^1095
/note="plant DNA end/T-DNA start"
mRNA complement(<1105..1811)
/note="octopine synthase (3' end)"
/partial
misc_feature 1569..1592
/note="24 bp border-like sequence"
misc_feature complement(1633..1634)
/note="major poly-A site"
misc_signal complement(1645..1650)
/note="poly-A signal"
misc_feature 1669..1692
/note="24 bp border-like sequence"
misc_signal complement(1681..1686)
/note="poly-A signal"
misc_feature complement(1756..1779)
/note="24 bp border-like sequence"
CDS complement(1985..2782)
/note="neomycin phosphotransferase (gtg start codon)"
misc_feature complement(2105..2128)
/note="24 bp border-like sequence"
misc_feature 2449..2472
/note="24 bp border-like sequence"
misc_feature complement(2462..2485)
/note="24 bp border-like sequence"
misc_recomb 2786^2787
/note="T-DNA end/plant DNA start"
misc_recomb 3044^3045
/note="T-DNA end/plant DNA start"
misc_recomb 3354^3355
/note="plant DNA end/T-DNA start"
misc_feature 3660..3683
/note="24 bp border-like sequence"
misc_feature 3875..3898
/note="24 bp border-like sequence"
CDS complement(4042..5013)
/note="streptomycin/spectinomycin adenyltransferase (gtg
start codon)"
CDS complement(4042..5009)
/note="streptomycin/spectinomycin adenyltransferase (gtg
start codon)"
/pseudo
misc_feature complement(4336..4359)
/note="24 bp border-like sequence"
misc_recomb 5585^5586
/note="T-DNA end/plant DNA start"
misc_recomb 5589^5590
/note="T-DNA end/plant DNA start"
misc_feature 5868..5891
/note="24 bp border-like sequence"
misc_feature 5872..5895
/note="24 bp border-like sequence"
misc_recomb 6389^6390
/note="T-DNA end/plant DNA start"
misc_recomb 6393^6394
/note="T-DNA end/plant DNA start"
misc_feature 6406..6429
/note="right T-DNA border"
BASE COUNT 1509 a 1754 c 1710 g 1452 t
ORIGIN
1 cggcaggata tattcaattg taaatggctt catgtccggg aaatctacat ggatcagcaa
61 tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt accaattttt tttcaattca
121 aaaatgtaga tgtccgcagc gttattataa aatgaaagta cattttgata aaacgacaaa
181 ttacgatccg tcgtatttat aggcgaaagc aataaacaaa ttattctaat tcggaaatct
241 ttatttcgac gtgtctacat tcacgtccaa atgggggctt agatgagaaa cttcacgatc
301 gatgccttga tttcgccatt cccagatacc catttcatct tcagattggt ctgagattat
361 gcgaaaatat acactcatat acataaatac tgacagtttg agctaccaat tcagtgtagc
421 ccattacctc acataattca ctcaaatgct aggcagtctg tcaactcggc gtcaatttgt
481 cggccactat acgatagttg cgcaaatttt caaagtcctg gcctaacatc acacctctgt
541 cggcggcggg tcccatttgt gataaatcca ccatcacaat agatagtcta atggacgaaa
601 aaggcgaata tttcgatgct gagattcgac gcaattaatt cgagaaaaat cccgtgattg
661 atgctgttga gttaccaata atatgggcag cgaaggccat ttaattataa gatcctgcaa
721 gcctcgtcgt cctggccgga ccacgctatc tgtgcaaggt ccccggcccc ggacgcgcgc
781 tccatgagca gagcgcccgc cgccgaggcg aagagtcggg cggcgccctg cccgtcccac
841 caggtcaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc
901 ggcatgtccc cctggcggac gggaagtatc cagctcgacc aaagcggcca tcgtgcctcc
961 ccactcctgc agttcggggg catggatgcg cggatagccg ctgctggttt cctggatgcc
1021 gacggatttg cactgccggt agaactccgc gaggtcgtcc agcctcaggc agcagctgaa
1081 ccaactcgcg aggggatcga gcccctgctg agcctcgaca tgttgtcgca aaattcgccc
1141 tggacccgcc caacgatttg tcgtcactgt caaggtttga cctgcacttc atttggggcc
1201 cacatacacc aaaaaaatgc tgcataattc tcggggcagc aagtcggtta cccggccgcc
1261 gtgctggacc gggttgaatg gtgcccgtaa ctttcggtag agcggacggc caatactcaa
1321 cttcaaggaa tctcacccat gcgcgccggc ggggaaccgg agttcccttc agtgaacgtt
1381 attagttcgc cgctcggtgt gtcgtagata ctagcccctg gggccttttg aaatttgaat
1441 aagatttatg taatcagtct tttaggtttg accggttctg ccgctttttt taaaattgga
1501 tttgtaataa taaaacgcaa ttgtttgtta ttgtggcgct ctatcataga tgtcgctata
1561 aacctattca gcacaatata ttgttttcat tttaatattg tacatataag tagtagggta
1621 caatcagtaa attgaacgga gaatattatt cataaaaata cgatagtaac gggtgatata
1681 ttcattagaa tgaaccgaaa ccggcggtaa ggatctgagc tacacatgct caggtttttt
1741 acaacgtgca caacagaatt gaaagcaaat atcatgcgat cataggcgtc tcgcatatct
1801 cattaaagca gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
1861 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
1921 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
1981 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
2041 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
2101 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
2161 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
2221 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
2281 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
2341 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
2401 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
2461 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
2521 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
2581 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
2641 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
2701 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
2761 atccatcttg ttcaatccac atgatcagat ctctaggcgc gtgggtgcgg acgtagtcag
2821 cgccattgcc gatcgcgtga agttccgccg caaggccgct ggacccagat cctttacagg
2881 aaggccaacg gtggcgccca agaaggattt ccgcgacacc gagaccaata gcggaagccc
2941 caacgccgac ttcagctttt gaaggttcga cagcacgtgc agcgatgttt ccggtgcggg
3001 gctcaagaaa aatcccatcc ccggatcgag gatgagccgg tcggcagcga ccccgctccg
3061 tcgcaaggcg gaaacccgcg cctcgaagaa ccgcacaatc tcgtcgagcg cgtcttcggg
3121 tcgaaggtga ccggtgcggg tggcgatgcc atcccctgcg ctgagtgcat aaccaccagc
3181 ctgcagtccg cctcagcaat atcgggatag agcgcagggt caggaaatcc ttggatatcg
3241 ttcaggtagc ccacgccgcg cttgagcgct agcgcgggtt tccggttgga agctgtcgat
3301 tgaaacacgg tgcatctgat cggacagggc gtctaagagc ggcgcaatac gtctgatctc
3361 atcggccggc gatacaggcc tcgcgtccgg atggctggcg gccggtccga catccacgac
3421 gtctgatccg actcgcagca tttcgaccgc cgcggtgaca gcgttggtgg ggtctagcag
3481 tacgtcaatc gaagaaggag tcctcggtga gattcagaat gccgaacacc gtcaccatgg
3541 cgtcggcctc cgcagcgact tccacgatgg ggatcgggcg agcaaaaagg cagcaattat
3601 gagccccata cctacaaagc cccacgcatc aagcttttga ccctgaagca actaggcaat
3661 ggctgtaatt atgacgacgc cgagtcccga accagactgc ataagcaaca accgacagaa
3721 tggatttcga aaccagagaa agaaaataaa tgcgatgcca taaccgatta tgaacaacgg
3781 cggaaggggc aagcttagta aatgcctcgc tagattttaa tgcggatgtt gcgattactt
3841 cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc ccaatttgtg
3901 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg
3961 tgagcaatta tgtgcttagt gcatctaatc gcttgagtta acgccggcga agcggcgtcg
4021 gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta
4081 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
4141 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
4201 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
4261 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
4321 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
4381 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
4441 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
4501 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
4561 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
4621 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
4681 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
4741 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
4801 agtcgatact tcggcgatca ccgcttccct catgatgttt aactttgttt tagggcgact
4861 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
4921 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
4981 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
5041 gcgtgaggcc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
5101 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
5161 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
5221 tcaggcattg gcggccttgc tgttcttcta cggcaagtgc tgtgcacgga tctgccctgg
5281 cttcaggaga tcggaagacc tcggccgtcc gggcgcttgc cggtggtgct gaccccggat
5341 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg
5401 tatggaacgg gcatgcggat cagtgagggt ttgcaactgc gggtcaagga ctggatttcg
5461 atcacggcac gatcatcgtg cgggagggca agggctccaa ggatcgggcc ttgatgttac
5521 ccgagagctt ggcacccagc ctgcgcgagc agctgtctcg tgcacgggca tggtggctga
5581 aggactaggc cgagggccgc agcggcgttg cgcttcccga cgcccttgag cggaagtatc
5641 cgcgcgccgg gcattcctgg ccgtggttct gggtttttgc gcagcacacg cattcgaccg
5701 atccacggag cggtgtcgtg cgtcgccatc acatgtatga ccagaccttt cagcgcgcct
5761 tcaaacgtgc cgtagaacaa gcaggcatca cgaagcccgc cacaccgcac accctccgcc
5821 actcgttcgc gacggccttg ctccgcagcg gttacgacat tcgaaccgtg caggatctgc
5881 tcggccattc cgacgtctct acgacgatga tttacacgca tgtgctgaaa gttggcggtg
5941 ccggagtgcg ctcaccgctt gatgcctgcc gcccctcact gtgagaggta gggcagcgca
6001 agtcaatcct agcggattca ctacccctgc gcgaaggcca tcggtgccgc atcgaacggc
6061 cggttgcgga aagtcctccc tgcgtccgct gatggccggc agcagcccgt cgttgaagga
6121 tccctgaaag cgacgttgga tgttaacatc tacaaattgc cttttcttac gaccatgtac
6181 gtaagcgctt acgtttttgg tggacccttg aggaaactgg tagctgttgt gggcctgtgg
6241 tctcaagatg gatcattaat ttccaccttc acctacgatg gggggcatcg caccggtgag
6301 taatattgta cggctaagag cgaatttggc ctgtagacct caattgcgag ctttctaatt
6361 tcaaactatt cgggcctaac ttttggtgtg atgatgctga ctggcaggat atataccgtt
6421 gtaat
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